This is a set of data and code underlying the thesis "Spatial analysis of multiple ecosystem service flows from natural and cultural landscapes in the Ethiopian highlands" and the latest related study "Modelling the Impact of Ecosystem Fragmentation on Ecosystem Services in the Degraded Ethiopian Highlands." The purpose of the study was to assess the impact of ecosystem fragmentation on critical ecosystem services, with a focus on data collected in 2019 and 2020. The field-based ecosystem services measurements capture data on three key services: grass biomass production, heat stress regulation, and crop pollination services across the study area. Social media geotagged photographs are used to estimate nature-based tourism services in the Lake Tana basin, with visitation rates serving as a proxy for cultural services data. Additionally, remote sensing-based products, including vegetation indices, land cover changes, and ecosystem fragmentation indices, provide crucial spatiotemporal input for modelling the effects of ecosystem fragmentation on ecosystem services in 2000 and 2020. The dataset is designed to provide a comprehensive understanding of how fragmentation has impacted these services, offering a basis for further analysis and decision-making in landscape management and conservation strategies in the Ethiopian Highlands.