Data underlying the publication: Model-based aberration corrected microscopy inside a glass tube



Data for the paper "Model-based aberration corrected microscopy inside a glass tube". Microscope objectives achieve near diffraction-limited performance only when used under the conditions they are designed for. In non-standard geometries, such as thick cover slips or curved surfaces, severe aberrations arise, inevitably impairing high-resolution imaging. Correcting such large aberrations using standard adaptive optics can be challenging: existing solutions are either not suited for strong aberrations, or require extensive feedback measurements, consequently taking a significant portion of the photon budget. We demonstrate that it is possible to pre-compute the corrections needed for high-resolution imaging inside a glass tube based on a priori information only. Our ray-tracing based method achieved over an order of magnitude increase in image contrast without the need for a feedback signal. Contents:1. 3-D 2PEF scans of fluorescent beads inside a glass tube.2. Brightfield microscopy images of the glass tube.3. Parameter scans of the phase correction patterns.4. Sensorless AO scans (Zernike mode scans).5. Ray-traced model-based phase correction patterns for glass tube.6. Code to recreate figures from the paper from raw data.7. Protocol to create tube samples.
Date made available29 Nov 2023
Publisher4TU.Centre for Research Data

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