3D bioprinting of a hyaluronan bioink through enzymatic-and visible light-crosslinking

D. Petta, A. R. Armiento, D. Grijpma, M. Alini, D. Eglin, M. D'Este (Corresponding Author)

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

Extrusion-based three-dimensional bioprinting relies on bioinks engineered to combine viscoelastic properties for extrusion and shape retention, and biological properties for cytocompatibility and tissue regeneration. To satisfy these conflicting requirements, bioinks often utilize either complex mixtures or complex modifications of biopolymers. In this paper we introduce and characterize a bioink exploiting a dual crosslinking mechanism, where an enzymatic reaction forms a soft gel suitable for cell encapsulation and extrusion, while a visible light photo-crosslinking allows shape retention of the printed construct. The influence of cell density and cell type on the rheological and printability properties was assessed correlating the printing outcomes with the damping factor, a rheological characteristic independent of the printing system. Stem cells, chondrocytes and fibroblasts were encapsulated, and their viability was assessed up to 14 days with live/dead, alamar blue and trypan blue assays. Additionally, the impact of the printing parameters on cell viability was investigated. Owing to its straightforward preparation, low modification, presence of two independent crosslinking mechanisms for tuning shear-thinning independently of the final shape fixation, the use of visible green instead of UV light, the possibility of encapsulating and sustaining the viability of different cell types, the hyaluronan bioink here presented is a valid biofabrication tool for producing 3D printed tissue-engineered constructs.

Original languageEnglish
Article number044104
JournalBiofabrication
Volume10
Issue number4
DOIs
Publication statusPublished - 25 Sep 2018

Fingerprint

Bioprinting
Printing
Hyaluronic Acid
Crosslinking
Extrusion
Light
Cell Survival
Cells
Tissue regeneration
Biopolymers
Trypan Blue
Shear thinning
Fibroblasts
Ultraviolet Rays
Chondrocytes
Stem cells
Complex Mixtures
Encapsulation
Ultraviolet radiation
Regeneration

Keywords

  • 3D printing
  • bioink
  • double gelation mechanism
  • hyaluronan
  • visible light curing

Cite this

Petta, D. ; Armiento, A. R. ; Grijpma, D. ; Alini, M. ; Eglin, D. ; D'Este, M. / 3D bioprinting of a hyaluronan bioink through enzymatic-and visible light-crosslinking. In: Biofabrication. 2018 ; Vol. 10, No. 4.
@article{94f81fbf325f48caa2333e41c2de0d23,
title = "3D bioprinting of a hyaluronan bioink through enzymatic-and visible light-crosslinking",
abstract = "Extrusion-based three-dimensional bioprinting relies on bioinks engineered to combine viscoelastic properties for extrusion and shape retention, and biological properties for cytocompatibility and tissue regeneration. To satisfy these conflicting requirements, bioinks often utilize either complex mixtures or complex modifications of biopolymers. In this paper we introduce and characterize a bioink exploiting a dual crosslinking mechanism, where an enzymatic reaction forms a soft gel suitable for cell encapsulation and extrusion, while a visible light photo-crosslinking allows shape retention of the printed construct. The influence of cell density and cell type on the rheological and printability properties was assessed correlating the printing outcomes with the damping factor, a rheological characteristic independent of the printing system. Stem cells, chondrocytes and fibroblasts were encapsulated, and their viability was assessed up to 14 days with live/dead, alamar blue and trypan blue assays. Additionally, the impact of the printing parameters on cell viability was investigated. Owing to its straightforward preparation, low modification, presence of two independent crosslinking mechanisms for tuning shear-thinning independently of the final shape fixation, the use of visible green instead of UV light, the possibility of encapsulating and sustaining the viability of different cell types, the hyaluronan bioink here presented is a valid biofabrication tool for producing 3D printed tissue-engineered constructs.",
keywords = "3D printing, bioink, double gelation mechanism, hyaluronan, visible light curing",
author = "D. Petta and Armiento, {A. R.} and D. Grijpma and M. Alini and D. Eglin and M. D'Este",
year = "2018",
month = "9",
day = "25",
doi = "10.1088/1758-5090/aadf58",
language = "English",
volume = "10",
journal = "Biofabrication",
issn = "1758-5082",
publisher = "IOP Publishing Ltd.",
number = "4",

}

3D bioprinting of a hyaluronan bioink through enzymatic-and visible light-crosslinking. / Petta, D.; Armiento, A. R.; Grijpma, D.; Alini, M.; Eglin, D.; D'Este, M. (Corresponding Author).

In: Biofabrication, Vol. 10, No. 4, 044104, 25.09.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - 3D bioprinting of a hyaluronan bioink through enzymatic-and visible light-crosslinking

AU - Petta, D.

AU - Armiento, A. R.

AU - Grijpma, D.

AU - Alini, M.

AU - Eglin, D.

AU - D'Este, M.

PY - 2018/9/25

Y1 - 2018/9/25

N2 - Extrusion-based three-dimensional bioprinting relies on bioinks engineered to combine viscoelastic properties for extrusion and shape retention, and biological properties for cytocompatibility and tissue regeneration. To satisfy these conflicting requirements, bioinks often utilize either complex mixtures or complex modifications of biopolymers. In this paper we introduce and characterize a bioink exploiting a dual crosslinking mechanism, where an enzymatic reaction forms a soft gel suitable for cell encapsulation and extrusion, while a visible light photo-crosslinking allows shape retention of the printed construct. The influence of cell density and cell type on the rheological and printability properties was assessed correlating the printing outcomes with the damping factor, a rheological characteristic independent of the printing system. Stem cells, chondrocytes and fibroblasts were encapsulated, and their viability was assessed up to 14 days with live/dead, alamar blue and trypan blue assays. Additionally, the impact of the printing parameters on cell viability was investigated. Owing to its straightforward preparation, low modification, presence of two independent crosslinking mechanisms for tuning shear-thinning independently of the final shape fixation, the use of visible green instead of UV light, the possibility of encapsulating and sustaining the viability of different cell types, the hyaluronan bioink here presented is a valid biofabrication tool for producing 3D printed tissue-engineered constructs.

AB - Extrusion-based three-dimensional bioprinting relies on bioinks engineered to combine viscoelastic properties for extrusion and shape retention, and biological properties for cytocompatibility and tissue regeneration. To satisfy these conflicting requirements, bioinks often utilize either complex mixtures or complex modifications of biopolymers. In this paper we introduce and characterize a bioink exploiting a dual crosslinking mechanism, where an enzymatic reaction forms a soft gel suitable for cell encapsulation and extrusion, while a visible light photo-crosslinking allows shape retention of the printed construct. The influence of cell density and cell type on the rheological and printability properties was assessed correlating the printing outcomes with the damping factor, a rheological characteristic independent of the printing system. Stem cells, chondrocytes and fibroblasts were encapsulated, and their viability was assessed up to 14 days with live/dead, alamar blue and trypan blue assays. Additionally, the impact of the printing parameters on cell viability was investigated. Owing to its straightforward preparation, low modification, presence of two independent crosslinking mechanisms for tuning shear-thinning independently of the final shape fixation, the use of visible green instead of UV light, the possibility of encapsulating and sustaining the viability of different cell types, the hyaluronan bioink here presented is a valid biofabrication tool for producing 3D printed tissue-engineered constructs.

KW - 3D printing

KW - bioink

KW - double gelation mechanism

KW - hyaluronan

KW - visible light curing

UR - http://www.scopus.com/inward/record.url?scp=85055769890&partnerID=8YFLogxK

U2 - 10.1088/1758-5090/aadf58

DO - 10.1088/1758-5090/aadf58

M3 - Article

VL - 10

JO - Biofabrication

JF - Biofabrication

SN - 1758-5082

IS - 4

M1 - 044104

ER -