3D Blood Vessels-on-Chip for atherosclerosis modelling

Heleen H.T. Middelkamp; Albert van den Berg; Andries D. van der Meer

3D Blood Vessels-on-Chip for atherosclerosis modelling

Heleen H.T. Middelkamp^*
, Albert van den Berg
, Andries D. van der Meer

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

2 Citations (Scopus)

583 Downloads (Pure)

Abstract

More than 25% of all deaths worldwide have a direct vascular cause, with coronary artery disease and stroke being two leading causes of death. These two diseases are the direct result of the acute rupture of atherosclerotic plaques in the arterial wall that contain dysfunctional tissue, fats and extracellular matrix. The disease processes in early atherosclerosis are hard to study due to the lack of realistic in vitro models. In this paper we present a 3D blood vessel-on-a-chip model for early atherosclerosis, which includes vascular tissue, cholesterol and immune cells in a patterned extracellular matrix. For the first time, we demonstrate that human iPSC-derived vascular cells take up oxidized LDL (‘bad cholesterol’) in a 3D microfluidic vessel on chip.

Original language	English
Title of host publication	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Subtitle of host publication	10-14 October 2021, Palm Springs, CA, USA
Pages	16931694
ISBN (Electronic)	9781733419031
Publication status	Published - 11 Oct 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 - Palm Springs, United States Duration: 10 Oct 2021 → 14 Oct 2021 Conference number: 25 https://microtas2021.org/

Publication series

Name	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021
Abbreviated title	MicroTAS 2021
Country/Territory	United States
City	Palm Springs
Period	10/10/21 → 14/10/21
Internet address	https://microtas2021.org/

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

atherosclerosis
blood vessel
organ on chip

Access to Document

PG1693Final published version, 862 KB

Cite this

Middelkamp, H. H. T., van den Berg, A., & van der Meer, A. D. (2021). 3D Blood Vessels-on-Chip for atherosclerosis modelling. In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10-14 October 2021, Palm Springs, CA, USA (pp. 16931694). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Middelkamp, Heleen H.T. ; van den Berg, Albert ; van der Meer, Andries D. / 3D Blood Vessels-on-Chip for atherosclerosis modelling. 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10-14 October 2021, Palm Springs, CA, USA. 2021. pp. 16931694 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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title = "3D Blood Vessels-on-Chip for atherosclerosis modelling",

abstract = "More than 25\% of all deaths worldwide have a direct vascular cause, with coronary artery disease and stroke being two leading causes of death. These two diseases are the direct result of the acute rupture of atherosclerotic plaques in the arterial wall that contain dysfunctional tissue, fats and extracellular matrix. The disease processes in early atherosclerosis are hard to study due to the lack of realistic in vitro models. In this paper we present a 3D blood vessel-on-a-chip model for early atherosclerosis, which includes vascular tissue, cholesterol and immune cells in a patterned extracellular matrix. For the first time, we demonstrate that human iPSC-derived vascular cells take up oxidized LDL ({\textquoteleft}bad cholesterol{\textquoteright}) in a 3D microfluidic vessel on chip.",

keywords = "atherosclerosis, blood vessel, organ on chip",

author = "Middelkamp, \{Heleen H.T.\} and \{van den Berg\}, Albert and \{van der Meer\}, \{Andries D.\}",

note = "Funding Information: This work was supported by the Netherlands Organ-on-Chip Initiative, an NWO Gravitation project (024.003.001) funded by the Ministry of Education, Culture and Science of the government of the Netherlands. Kim Vermeul is acknowledged for differentiating the iPSC-EC needed for this project. Mees de Graaf is acknowledged for his help in first creating the viscous finger patterned blood vessels. Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

month = oct,

day = "11",

language = "English",

isbn = "978-1-7334190-3-1",

series = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

pages = "16931694",

booktitle = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

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Middelkamp, HHT, van den Berg, A & van der Meer, AD 2021, 3D Blood Vessels-on-Chip for atherosclerosis modelling. in 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10-14 October 2021, Palm Springs, CA, USA. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp. 16931694, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021, Palm Springs, California, United States, 10/10/21.

3D Blood Vessels-on-Chip for atherosclerosis modelling. / Middelkamp, Heleen H.T.; van den Berg, Albert ; van der Meer, Andries D.
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 10-14 October 2021, Palm Springs, CA, USA. 2021. p. 16931694 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - 3D Blood Vessels-on-Chip for atherosclerosis modelling

AU - Middelkamp, Heleen H.T.

AU - van den Berg, Albert

AU - van der Meer, Andries D.

N1 - Conference code: 25

PY - 2021/10/11

Y1 - 2021/10/11

N2 - More than 25% of all deaths worldwide have a direct vascular cause, with coronary artery disease and stroke being two leading causes of death. These two diseases are the direct result of the acute rupture of atherosclerotic plaques in the arterial wall that contain dysfunctional tissue, fats and extracellular matrix. The disease processes in early atherosclerosis are hard to study due to the lack of realistic in vitro models. In this paper we present a 3D blood vessel-on-a-chip model for early atherosclerosis, which includes vascular tissue, cholesterol and immune cells in a patterned extracellular matrix. For the first time, we demonstrate that human iPSC-derived vascular cells take up oxidized LDL (‘bad cholesterol’) in a 3D microfluidic vessel on chip.

AB - More than 25% of all deaths worldwide have a direct vascular cause, with coronary artery disease and stroke being two leading causes of death. These two diseases are the direct result of the acute rupture of atherosclerotic plaques in the arterial wall that contain dysfunctional tissue, fats and extracellular matrix. The disease processes in early atherosclerosis are hard to study due to the lack of realistic in vitro models. In this paper we present a 3D blood vessel-on-a-chip model for early atherosclerosis, which includes vascular tissue, cholesterol and immune cells in a patterned extracellular matrix. For the first time, we demonstrate that human iPSC-derived vascular cells take up oxidized LDL (‘bad cholesterol’) in a 3D microfluidic vessel on chip.

KW - atherosclerosis

KW - blood vessel

KW - organ on chip

UR - https://www.scopus.com/pages/publications/85136941530

M3 - Conference contribution

AN - SCOPUS:85136941530

SN - 978-1-7334190-3-1

T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 16931694

BT - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

3D Blood Vessels-on-Chip for atherosclerosis modelling

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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