3D Printed Cartilage-Like Tissue Constructs with Spatially Controlled Mechanical Properties

Bruna A.G. de Melo, Yasamin A. Jodat, Shreya Mehrotra, Michelle A. Calabrese, Tom Kamperman, Biman B. Mandal, Maria H.A. Santana, Eben Alsberg, Jeroen Leijten, Su Ryon Shin

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Developing biomimetic cartilaginous tissues that support locomotion while maintaining chondrogenic behavior is a major challenge in the tissue engineering field. Specifically, while locomotive forces demand tissues with strong mechanical properties, chondrogenesis requires a soft microenvironment. To address this challenge, 3D cartilage-like tissue is fabricated using two biomaterials with different mechanical properties: a hard biomaterial to reflect the macromechanical properties of native cartilage, and a soft biomaterial to create a chondrogenic microenvironment. To this end, a bath composed of an interpenetrating polymer network (IPN) of polyethylene glycol (PEG) and alginate hydrogel (MPa order compressive modulus) is developed as an extracellular matrix (ECM) with self-healing properties. Within this bath supplemented with thrombin, human mesenchymal stem cell (hMSC) spheroids embedded in fibrinogen are 3D bioprinted, creating a soft microenvironment composed of fibrin (kPa order compressive modulus) that simulate cartilage's pericellular matrix and allow a fast diffusion of nutrients. The bioprinted hMSC spheroids present high viability and chondrogenic-like behavior without adversely affecting the macromechanical properties of the tissue. Therefore, the ability to locally bioprint a soft and cell stimulating biomaterial inside of a mechanically robust hydrogel is demonstrated, thereby uncoupling the micro- and macromechanical properties of the 3D printed tissues such as cartilage.

Original languageEnglish
Article number1906330
JournalAdvanced functional materials
DOIs
Publication statusE-pub ahead of print/First online - 21 Oct 2019

Fingerprint

cartilage
Cartilage
Biocompatible Materials
Biomaterials
mechanical properties
Tissue
Mechanical properties
stem cells
Hydrogel
spheroids
Stem cells
Hydrogels
baths
locomotives
fibrin
thrombin
fibrinogen
Interpenetrating polymer networks
locomotion
Locomotives

Keywords

  • UT-Hybrid-D
  • cartilage
  • fibrin
  • IPN
  • spheroids
  • bioprinting

Cite this

de Melo, B. A. G., Jodat, Y. A., Mehrotra, S., Calabrese, M. A., Kamperman, T., Mandal, B. B., ... Shin, S. R. (2019). 3D Printed Cartilage-Like Tissue Constructs with Spatially Controlled Mechanical Properties. Advanced functional materials, [1906330]. https://doi.org/10.1002/adfm.201906330
de Melo, Bruna A.G. ; Jodat, Yasamin A. ; Mehrotra, Shreya ; Calabrese, Michelle A. ; Kamperman, Tom ; Mandal, Biman B. ; Santana, Maria H.A. ; Alsberg, Eben ; Leijten, Jeroen ; Shin, Su Ryon. / 3D Printed Cartilage-Like Tissue Constructs with Spatially Controlled Mechanical Properties. In: Advanced functional materials. 2019.
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3D Printed Cartilage-Like Tissue Constructs with Spatially Controlled Mechanical Properties. / de Melo, Bruna A.G.; Jodat, Yasamin A.; Mehrotra, Shreya; Calabrese, Michelle A.; Kamperman, Tom; Mandal, Biman B.; Santana, Maria H.A.; Alsberg, Eben; Leijten, Jeroen; Shin, Su Ryon.

In: Advanced functional materials, 21.10.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - de Melo, Bruna A.G.

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AU - Mehrotra, Shreya

AU - Calabrese, Michelle A.

AU - Kamperman, Tom

AU - Mandal, Biman B.

AU - Santana, Maria H.A.

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AU - Shin, Su Ryon

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AB - Developing biomimetic cartilaginous tissues that support locomotion while maintaining chondrogenic behavior is a major challenge in the tissue engineering field. Specifically, while locomotive forces demand tissues with strong mechanical properties, chondrogenesis requires a soft microenvironment. To address this challenge, 3D cartilage-like tissue is fabricated using two biomaterials with different mechanical properties: a hard biomaterial to reflect the macromechanical properties of native cartilage, and a soft biomaterial to create a chondrogenic microenvironment. To this end, a bath composed of an interpenetrating polymer network (IPN) of polyethylene glycol (PEG) and alginate hydrogel (MPa order compressive modulus) is developed as an extracellular matrix (ECM) with self-healing properties. Within this bath supplemented with thrombin, human mesenchymal stem cell (hMSC) spheroids embedded in fibrinogen are 3D bioprinted, creating a soft microenvironment composed of fibrin (kPa order compressive modulus) that simulate cartilage's pericellular matrix and allow a fast diffusion of nutrients. The bioprinted hMSC spheroids present high viability and chondrogenic-like behavior without adversely affecting the macromechanical properties of the tissue. Therefore, the ability to locally bioprint a soft and cell stimulating biomaterial inside of a mechanically robust hydrogel is demonstrated, thereby uncoupling the micro- and macromechanical properties of the 3D printed tissues such as cartilage.

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