3D Printing for Microgel-Based Liver Cell Encapsulation

Jonathan S. O'Connor, Heesoo Kim, Eunheui Gwag, Leon Abelmann, Baeckkyoung Sung, Andreas Manz

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

1 Citation (Scopus)
15 Downloads (Pure)

Abstract

In this work, we describe the rapid prototyping of a microfluidic device for the surfactant free encapsulation of human liver cells (HepG2 cell line) in gelatin microgels, for the purpose of 3D tissue mimics in high-throughput cytotoxicity screening. Chips with rectangular channels of approximately 260 μm high by 350 μm wide produced a droplet size of 130±12 μm at a rate of 7.9±0.6 drops per second. Integrated water heating and cooling systems were efficient at regulating channel temperature, preventing the coalescence of droplets within the device without any need for surfactants. HepG2 cell viability two hours after microgel generation was 96.5%.

Original languageEnglish
Title of host publication34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
Place of PublicationPiscataway, NJ
PublisherIEEE
Pages1023-1026
Number of pages4
ISBN (Electronic)978-1-6654-3024-1
ISBN (Print)978-1-6654-1912-3
DOIs
Publication statusPublished - 15 Mar 2021
EventIEEE 34th International Conference on Micro Electro Mechanical Systems, MEMS 2021 - Online, United States
Duration: 25 Jan 202129 Jan 2021
Conference number: 34
https://www.mems21.org/

Publication series

NameIEEE International Conference on Micro Electro Mechanical Systems (MEMS)
PublisherIEEE
Volume2021
ISSN (Print)1084-6999
ISSN (Electronic)2160-1968

Conference

ConferenceIEEE 34th International Conference on Micro Electro Mechanical Systems, MEMS 2021
Abbreviated titleMEMS 2021
Country/TerritoryUnited States
CityOnline
Period25/01/2129/01/21
Internet address

Keywords

  • 3D Printing
  • Additive manufacturing
  • Cell-laden gels
  • droplet generation
  • Microfluidics
  • Soft lithography

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