3D Printing for Microgel-Based Liver Cell Encapsulation

Jonathan S. O'Connor; Heesoo Kim; Eunheui Gwag; Leon Abelmann; Baeckkyoung Sung; Andreas Manz

doi:10.1109/MEMS51782.2021.9375385

3D Printing for Microgel-Based Liver Cell Encapsulation

Jonathan S. O'Connor, Heesoo Kim, Eunheui Gwag, Leon Abelmann, Baeckkyoung Sung, Andreas Manz

Robotics and Mechatronics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

3 Citations (Scopus)

99 Downloads (Pure)

Abstract

In this work, we describe the rapid prototyping of a microfluidic device for the surfactant free encapsulation of human liver cells (HepG2 cell line) in gelatin microgels, for the purpose of 3D tissue mimics in high-throughput cytotoxicity screening. Chips with rectangular channels of approximately 260 μm high by 350 μm wide produced a droplet size of 130±12 μm at a rate of 7.9±0.6 drops per second. Integrated water heating and cooling systems were efficient at regulating channel temperature, preventing the coalescence of droplets within the device without any need for surfactants. HepG2 cell viability two hours after microgel generation was 96.5%.

Original language	English
Title of host publication	34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
Place of Publication	Piscataway, NJ
Publisher	IEEE
Pages	1023-1026
Number of pages	4
ISBN (Electronic)	978-1-6654-3024-1
ISBN (Print)	978-1-6654-1912-3
DOIs	https://doi.org/10.1109/MEMS51782.2021.9375385
Publication status	Published - 15 Mar 2021
Event	IEEE 34th International Conference on Micro Electro Mechanical Systems, MEMS 2021 - Online, United States Duration: 25 Jan 2021 → 29 Jan 2021 Conference number: 34 https://www.mems21.org/

Publication series

Name	IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Publisher	IEEE
Volume	2021
ISSN (Print)	1084-6999
ISSN (Electronic)	2160-1968

Conference

Conference	IEEE 34th International Conference on Micro Electro Mechanical Systems, MEMS 2021
Abbreviated title	MEMS 2021
Country/Territory	United States
City	Online
Period	25/01/21 → 29/01/21
Internet address	https://www.mems21.org/

Keywords

2022 OA procedure
Additive manufacturing
Cell-laden gels
droplet generation
Microfluidics
Soft lithography
3D Printing

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/MEMS51782.2021.9375385

O'Connor2021printingFinal published version, 1.73 MBLicence: Taverne

Cite this

O'Connor, J. S., Kim, H., Gwag, E., Abelmann, L., Sung, B., & Manz, A. (2021). 3D Printing for Microgel-Based Liver Cell Encapsulation. In 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 (pp. 1023-1026). Article 9375385 (IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2021). IEEE. https://doi.org/10.1109/MEMS51782.2021.9375385

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title = "3D Printing for Microgel-Based Liver Cell Encapsulation",

abstract = "In this work, we describe the rapid prototyping of a microfluidic device for the surfactant free encapsulation of human liver cells (HepG2 cell line) in gelatin microgels, for the purpose of 3D tissue mimics in high-throughput cytotoxicity screening. Chips with rectangular channels of approximately 260 μm high by 350 μm wide produced a droplet size of 130±12 μm at a rate of 7.9±0.6 drops per second. Integrated water heating and cooling systems were efficient at regulating channel temperature, preventing the coalescence of droplets within the device without any need for surfactants. HepG2 cell viability two hours after microgel generation was 96.5%.",

keywords = "2022 OA procedure, Additive manufacturing, Cell-laden gels, droplet generation, Microfluidics, Soft lithography, 3D Printing",

author = "O'Connor, {Jonathan S.} and Heesoo Kim and Eunheui Gwag and Leon Abelmann and Baeckkyoung Sung and Andreas Manz",

year = "2021",

month = mar,

day = "15",

doi = "10.1109/MEMS51782.2021.9375385",

language = "English",

isbn = "978-1-6654-1912-3",

series = "IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "IEEE",

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booktitle = "34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021",

address = "United States",

note = "IEEE 34th International Conference on Micro Electro Mechanical Systems, MEMS 2021, MEMS 2021 ; Conference date: 25-01-2021 Through 29-01-2021",

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O'Connor, JS, Kim, H, Gwag, E, Abelmann, L, Sung, B & Manz, A 2021, 3D Printing for Microgel-Based Liver Cell Encapsulation. in 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021., 9375385, IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2021, IEEE, Piscataway, NJ, pp. 1023-1026, IEEE 34th International Conference on Micro Electro Mechanical Systems, MEMS 2021, Online, United States, 25/01/21. https://doi.org/10.1109/MEMS51782.2021.9375385

3D Printing for Microgel-Based Liver Cell Encapsulation. / O'Connor, Jonathan S.; Kim, Heesoo; Gwag, Eunheui et al.
34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021. Piscataway, NJ: IEEE, 2021. p. 1023-1026 9375385 (IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2021).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - 3D Printing for Microgel-Based Liver Cell Encapsulation

AU - O'Connor, Jonathan S.

AU - Kim, Heesoo

AU - Gwag, Eunheui

AU - Abelmann, Leon

AU - Sung, Baeckkyoung

AU - Manz, Andreas

N1 - Conference code: 34

PY - 2021/3/15

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N2 - In this work, we describe the rapid prototyping of a microfluidic device for the surfactant free encapsulation of human liver cells (HepG2 cell line) in gelatin microgels, for the purpose of 3D tissue mimics in high-throughput cytotoxicity screening. Chips with rectangular channels of approximately 260 μm high by 350 μm wide produced a droplet size of 130±12 μm at a rate of 7.9±0.6 drops per second. Integrated water heating and cooling systems were efficient at regulating channel temperature, preventing the coalescence of droplets within the device without any need for surfactants. HepG2 cell viability two hours after microgel generation was 96.5%.

AB - In this work, we describe the rapid prototyping of a microfluidic device for the surfactant free encapsulation of human liver cells (HepG2 cell line) in gelatin microgels, for the purpose of 3D tissue mimics in high-throughput cytotoxicity screening. Chips with rectangular channels of approximately 260 μm high by 350 μm wide produced a droplet size of 130±12 μm at a rate of 7.9±0.6 drops per second. Integrated water heating and cooling systems were efficient at regulating channel temperature, preventing the coalescence of droplets within the device without any need for surfactants. HepG2 cell viability two hours after microgel generation was 96.5%.

KW - 2022 OA procedure

KW - Additive manufacturing

KW - Cell-laden gels

KW - droplet generation

KW - Microfluidics

KW - Soft lithography

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T2 - IEEE 34th International Conference on Micro Electro Mechanical Systems, MEMS 2021

Y2 - 25 January 2021 through 29 January 2021

ER -

3D Printing for Microgel-Based Liver Cell Encapsulation

Abstract

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Conference

Keywords

UN SDGs

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