3D Printing for Microgel-Based Liver Cell Encapsulation

Jonathan S. O'Connor, Heesoo Kim, Eunheui Gwag, Leon Abelmann, Baeckkyoung Sung, Andreas Manz

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

In this work, we describe the rapid prototyping of a microfluidic device for the surfactant free encapsulation of human liver cells (HepG2 cell line) in gelatin microgels, for the purpose of 3D tissue mimics in high-throughput cytotoxicity screening. Chips with rectangular channels of approximately 260 μm high by 350 μm wide produced a droplet size of 130±12 μm at a rate of 7.9±0.6 drops per second. Integrated water heating and cooling systems were efficient at regulating channel temperature, preventing the coalescence of droplets within the device without any need for surfactants. HepG2 cell viability two hours after microgel generation was 96.5%.

Original languageEnglish
Title of host publication34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
PublisherIEEE
Pages1023-1026
Number of pages4
ISBN (Electronic)9781665419123
DOIs
Publication statusPublished - 25 Jan 2021
Externally publishedYes
Event34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 - Virtual, Gainesville, United States
Duration: 25 Jan 202129 Jan 2021

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2021-January
ISSN (Print)1084-6999

Conference

Conference34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
CountryUnited States
CityVirtual, Gainesville
Period25/01/2129/01/21

Keywords

  • 3D Printing
  • Additive manufacturing
  • Cell-laden gels
  • droplet generation
  • Microfluidics
  • Soft lithography

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