4D Biomaterials for Light-Guided Angiogenesis

Aleeza Farrukh; Julieta I. Paez; Aránzazu del Campo

doi:10.1002/adfm.201807734

4D Biomaterials for Light-Guided Angiogenesis

Aleeza Farrukh, Julieta I. Paez, Aránzazu del Campo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

48 Citations (Scopus)

Abstract

A strategy for spatial and temporal regulation of ligand presentation within a biomaterial, and the consequent site- and time-specific cellular responses in 4D cell cultures are presented. The key molecular component is a light-activatable adhesive peptidomimetic (cyclo Arg-Gly-Asp-phe-Cys, RGDfC) modified with the two-photon photocleavable group (p-methoxynitrobiphenyl, PMNB) used to functionalize a hydrogel. A scanning laser at 740 nm defines the 4D presentation of active RGD ligands within the gel, and directs basic cellular processes of embedded cells in situ. The excellent photochemical properties of the PMNB photoremovable group allows direct photomanipulation of the cellular environment without appreciable damage of the embedded cells. Light-directed migration of fibroblasts within a crosslinked poly(ethylene glycol) (PEG) hydrogel, and sequential, light-regulated angiogenesis with human umbilical vein endothelial cells (HUVECs) in 4D constructs is demonstrated. The materials presented here represent unique microenvironments to reconstruct dynamic changes in the composition of the extracellular space of cells that occur in in vivo tissues.

Original language	English
Article number	1807734
Journal	Advanced functional materials
Volume	29
Issue number	6
DOIs	https://doi.org/10.1002/adfm.201807734
Publication status	Published - 8 Feb 2019
Externally published	Yes

Keywords

angiogenesis
cell migration
dynamic biomaterial
photoactivatable RGD
two-photon phototriggers
n/a OA procedure

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10.1002/adfm.201807734

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title = "4D Biomaterials for Light-Guided Angiogenesis",

abstract = "A strategy for spatial and temporal regulation of ligand presentation within a biomaterial, and the consequent site- and time-specific cellular responses in 4D cell cultures are presented. The key molecular component is a light-activatable adhesive peptidomimetic (cyclo Arg-Gly-Asp-phe-Cys, RGDfC) modified with the two-photon photocleavable group (p-methoxynitrobiphenyl, PMNB) used to functionalize a hydrogel. A scanning laser at 740 nm defines the 4D presentation of active RGD ligands within the gel, and directs basic cellular processes of embedded cells in situ. The excellent photochemical properties of the PMNB photoremovable group allows direct photomanipulation of the cellular environment without appreciable damage of the embedded cells. Light-directed migration of fibroblasts within a crosslinked poly(ethylene glycol) (PEG) hydrogel, and sequential, light-regulated angiogenesis with human umbilical vein endothelial cells (HUVECs) in 4D constructs is demonstrated. The materials presented here represent unique microenvironments to reconstruct dynamic changes in the composition of the extracellular space of cells that occur in in vivo tissues.",

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author = "Aleeza Farrukh and Paez, {Julieta I.} and {del Campo}, Ar{\'a}nzazu",

note = "Funding Information: A.F. thanks the financial support from the Max-Planck Graduate Center during her PhD program. Funding from the SFB1027 Collective Research Center and the European Union{\textquoteright}s Horizon 2020 research and innovation programme under the FET PROACTIVE grant agreement no. 731957 (Mechano-Control) is gratefully acknowledged. Publisher Copyright: {\textcopyright} 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

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4D Biomaterials for Light-Guided Angiogenesis

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Keywords

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