A 3D Toolbox to Enhance Physiological Relevance of Human Tissue Models

Nathalie Picollet-D'hahan, Monika E. Dolega, Lavinia Liguori, Christophe Marquette, Séverine Le Gac, Xavier Gidrol*, Donald K. Martin

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

52 Citations (Scopus)
54 Downloads (Pure)


We discuss the current challenges and future prospects of flow-based organoid models and 3D self-assembling scaffolds. The existing paradigm of 3D culture suffers from a lack of control over organoid size and shape; can be an obstacle for cell harvesting and extended cellular and molecular analysis; and does not provide access to the function of exocrine glands. Moreover, existing organ-on-chip models are mostly composed of 2D extracellular matrix (ECM)-coated elastomeric membranes that do not mimic real organ architectures. A new comprehensive 3D toolbox for cell biology has emerged to address some of these issues. Advances in microfabrication and cell-culturing approaches enable the engineering of sophisticated models that mimic organ 3D architectures and physiological conditions, while supporting flow-based drug screening and secretomics-based diagnosis.

Original languageEnglish
Pages (from-to)757-769
Number of pages13
JournalTrends in biotechnology
Issue number9
Publication statusPublished - 1 Sept 2016


  • 3D models
  • 3D scaffolds
  • Microfluidics
  • Microtechnologies
  • Organs-on-chips
  • 2023 OA procedure


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