A cheap 2D fluorescence detection system for µM-sized beads on-Chip

Loes Irene Segerink; Maarten J. Koster; A.J. Sprenkels; I. Vermes; Albert van den Berg

A cheap 2D fluorescence detection system for µM-sized beads on-Chip

Loes Irene Segerink, Maarten J. Koster, A.J. Sprenkels, I. Vermes, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

In this paper a compact fluorescence detection system for on-chip analysis of μm-sized beads is presented. The system comprises a cheap optical pickup with integrated functionalities that are used for the detection. Furthermore some other optical components are added for the fluorescence detection. Another part of the system is the microfluidic chip which contains liquid channels and optical markers used for the autofocus and channel find algorithms. Within one minute the channel of interest is found and fluorescent beads are detected in the microchannel without the need for dynamic focusing, since a twodimensional scan across the channel width is performed.

Original language	English
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011
Place of Publication	Washington
Publisher	The Chemical and Biological Microsystems Society
Pages	556-558
Number of pages	3
ISBN (Print)	978-0-979-8064-4-5
Publication status	Published - 2 Oct 2011
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011 - Seattle, United States Duration: 2 Oct 2011 → 6 Oct 2011 Conference number: 15 https://microtasconferences.org/microtas2011/

Publication series

Name
Publisher	Chemical and Biological Microsystems Society
ISSN (Print)	1556-5904

Conference

Conference	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011
Abbreviated title	MicroTAS
Country/Territory	United States
City	Seattle
Period	2/10/11 → 6/10/11
Internet address	https://microtasconferences.org/microtas2011/

Cite this

@inproceedings{05e86d6a740847d5ab98885012f6bc31,

title = "A cheap 2D fluorescence detection system for µM-sized beads on-Chip",

abstract = "In this paper a compact fluorescence detection system for on-chip analysis of μm-sized beads is presented. The system comprises a cheap optical pickup with integrated functionalities that are used for the detection. Furthermore some other optical components are added for the fluorescence detection. Another part of the system is the microfluidic chip which contains liquid channels and optical markers used for the autofocus and channel find algorithms. Within one minute the channel of interest is found and fluorescent beads are detected in the microchannel without the need for dynamic focusing, since a twodimensional scan across the channel width is performed.",

author = "Segerink, {Loes Irene} and Koster, {Maarten J.} and A.J. Sprenkels and I. Vermes and {van den Berg}, Albert",

year = "2011",

month = oct,

day = "2",

language = "English",

isbn = "978-0-979-8064-4-5",

publisher = "The Chemical and Biological Microsystems Society",

pages = "556--558",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011, MicroTAS ; Conference date: 02-10-2011 Through 06-10-2011",

url = "https://microtasconferences.org/microtas2011/",

}

Segerink, LI, Koster, MJ, Sprenkels, AJ, Vermes, I & van den Berg, A 2011, A cheap 2D fluorescence detection system for µM-sized beads on-Chip. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011. The Chemical and Biological Microsystems Society, Washington, pp. 556-558, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011, Seattle, Washington, United States, 2/10/11.

A cheap 2D fluorescence detection system for µM-sized beads on-Chip. / Segerink, Loes Irene; Koster, Maarten J.; Sprenkels, A.J. et al.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011. Washington : The Chemical and Biological Microsystems Society, 2011. p. 556-558.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - A cheap 2D fluorescence detection system for µM-sized beads on-Chip

AU - Segerink, Loes Irene

AU - Koster, Maarten J.

AU - Sprenkels, A.J.

AU - Vermes, I.

AU - van den Berg, Albert

N1 - Conference code: 15

PY - 2011/10/2

Y1 - 2011/10/2

N2 - In this paper a compact fluorescence detection system for on-chip analysis of μm-sized beads is presented. The system comprises a cheap optical pickup with integrated functionalities that are used for the detection. Furthermore some other optical components are added for the fluorescence detection. Another part of the system is the microfluidic chip which contains liquid channels and optical markers used for the autofocus and channel find algorithms. Within one minute the channel of interest is found and fluorescent beads are detected in the microchannel without the need for dynamic focusing, since a twodimensional scan across the channel width is performed.

AB - In this paper a compact fluorescence detection system for on-chip analysis of μm-sized beads is presented. The system comprises a cheap optical pickup with integrated functionalities that are used for the detection. Furthermore some other optical components are added for the fluorescence detection. Another part of the system is the microfluidic chip which contains liquid channels and optical markers used for the autofocus and channel find algorithms. Within one minute the channel of interest is found and fluorescent beads are detected in the microchannel without the need for dynamic focusing, since a twodimensional scan across the channel width is performed.

M3 - Conference contribution

SN - 978-0-979-8064-4-5

SP - 556

EP - 558

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011

PB - The Chemical and Biological Microsystems Society

CY - Washington

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

A cheap 2D fluorescence detection system for µM-sized beads on-Chip

Abstract

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Conference

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