A cheap 2D fluorescence detection system for μM-sized beads on-chip

Loes I. Segerink; Maarten J. Koster; Ad J. Sprenkels; Istvan Vermes; Albert Van Den Berg

A cheap 2D fluorescence detection system for μM-sized beads on-chip

Loes I. Segerink, Maarten J. Koster, Ad J. Sprenkels, Istvan Vermes, Albert Van Den Berg

Biomedical and Environmental Sensorsystems

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

3 Citations (Scopus)

71 Downloads (Pure)

Abstract

In this paper a compact fluorescence detection system for on-chip analysis of μm-sized beads is presented. The system comprises a cheap optical pickup with integrated functionalities that are used for the detection. Furthermore some other optical components are added for the fluorescence detection. Another part of the system is the microfluidic chip which contains liquid channels and optical markers used for the autofocus and channel find algorithms. Within one minute the channel of interest is found and fluorescent beads are detected in the microchannel without the need for dynamic focusing, since a two-dimensional scan across the channel width is performed.

Original language	English
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages	556-558
Number of pages	3
Publication status	Published - 1 Dec 2011
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011 - Seattle, United States Duration: 2 Oct 2011 → 6 Oct 2011 Conference number: 15 https://microtasconferences.org/microtas2011/

Conference

Conference	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011
Abbreviated title	MicroTAS
Country/Territory	United States
City	Seattle
Period	2/10/11 → 6/10/11
Internet address	https://microtasconferences.org/microtas2011/

Keywords

2D
Beads
Detection
Fluorescence
Microfluidic chip
Optical markers

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Segerink2011cheapFinal published version, 334 KB

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@inproceedings{19f944d8fed54dcbb4891b14eb63d27e,

title = "A cheap 2D fluorescence detection system for μM-sized beads on-chip",

abstract = "In this paper a compact fluorescence detection system for on-chip analysis of μm-sized beads is presented. The system comprises a cheap optical pickup with integrated functionalities that are used for the detection. Furthermore some other optical components are added for the fluorescence detection. Another part of the system is the microfluidic chip which contains liquid channels and optical markers used for the autofocus and channel find algorithms. Within one minute the channel of interest is found and fluorescent beads are detected in the microchannel without the need for dynamic focusing, since a two-dimensional scan across the channel width is performed.",

keywords = "2D, Beads, Detection, Fluorescence, Microfluidic chip, Optical markers",

author = "Segerink, {Loes I.} and Koster, {Maarten J.} and Sprenkels, {Ad J.} and Istvan Vermes and {Van Den Berg}, Albert",

year = "2011",

month = dec,

day = "1",

language = "English",

isbn = "9781618395955",

pages = "556--558",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011, MicroTAS ; Conference date: 02-10-2011 Through 06-10-2011",

url = "https://microtasconferences.org/microtas2011/",

}

Segerink, LI, Koster, MJ, Sprenkels, AJ, Vermes, I & Van Den Berg, A 2011, A cheap 2D fluorescence detection system for μM-sized beads on-chip. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. pp. 556-558, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011, Seattle, Washington, United States, 2/10/11.

A cheap 2D fluorescence detection system for μM-sized beads on-chip. / Segerink, Loes I.; Koster, Maarten J.; Sprenkels, Ad J. et al.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 556-558.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - A cheap 2D fluorescence detection system for μM-sized beads on-chip

AU - Segerink, Loes I.

AU - Koster, Maarten J.

AU - Sprenkels, Ad J.

AU - Vermes, Istvan

AU - Van Den Berg, Albert

N1 - Conference code: 15

PY - 2011/12/1

Y1 - 2011/12/1

N2 - In this paper a compact fluorescence detection system for on-chip analysis of μm-sized beads is presented. The system comprises a cheap optical pickup with integrated functionalities that are used for the detection. Furthermore some other optical components are added for the fluorescence detection. Another part of the system is the microfluidic chip which contains liquid channels and optical markers used for the autofocus and channel find algorithms. Within one minute the channel of interest is found and fluorescent beads are detected in the microchannel without the need for dynamic focusing, since a two-dimensional scan across the channel width is performed.

AB - In this paper a compact fluorescence detection system for on-chip analysis of μm-sized beads is presented. The system comprises a cheap optical pickup with integrated functionalities that are used for the detection. Furthermore some other optical components are added for the fluorescence detection. Another part of the system is the microfluidic chip which contains liquid channels and optical markers used for the autofocus and channel find algorithms. Within one minute the channel of interest is found and fluorescent beads are detected in the microchannel without the need for dynamic focusing, since a two-dimensional scan across the channel width is performed.

KW - 2D

KW - Beads

KW - Detection

KW - Fluorescence

KW - Microfluidic chip

KW - Optical markers

UR - http://www.scopus.com/inward/record.url?scp=84883812821&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84883812821

SN - 9781618395955

SP - 556

EP - 558

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

A cheap 2D fluorescence detection system for μM-sized beads on-chip

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Conference

Keywords

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