TY - JOUR
T1 - A comparison of breast cancer tumor cells with varying expression of the Her2/neu receptor by Raman microspectroscopic imaging
AU - Hartsuiker, Liesbeth
AU - Zeijen, Nicole J.L.
AU - Terstappen, Leon W.M.M.
AU - Otto, Cees
PY - 2010
Y1 - 2010
N2 - The Her2/neu proto-oncogene is amplified in 25 to 30 percent of human primary breast carcinomas. The roles of Her2/neu have been reported before in literature, showing different relations to intracellular lipid composition. Here, we use Raman microspectroscopic imaging to reveal the chemical composition of single live cells from breast carcinoma cell lines MDA-MB-231, MDA-MB-435s and SK-BR-3, which express Her2/neu receptor in different extent. Average Raman spectra of the different cell populations show prominent lipid presence in all cell lines. With high significance, Raman difference spectra reveal increased lipid contents, as well as a lower degree of fatty acid saturation in the MDA-MB cell lines with respect to the SK-BR-3 cells. These results are confirmed by hierarchical cluster analysis of single cells. High internal consistency of the chemical compositions in the cell lines is shown by hierarchical cluster analysis on a single matrix composed of the data of different cells from a single cell line. Although Her2/neu expression is highest for SK-BR-3 cells, their lipid contents are lower than that of the MDA-MB cell lines, which express less to no Her2/neu receptors. Rather than metabolic rate or senescence, the degree of metastaticity of the cells appears to be related to the polyunsaturated fatty acid contents of the cells.
AB - The Her2/neu proto-oncogene is amplified in 25 to 30 percent of human primary breast carcinomas. The roles of Her2/neu have been reported before in literature, showing different relations to intracellular lipid composition. Here, we use Raman microspectroscopic imaging to reveal the chemical composition of single live cells from breast carcinoma cell lines MDA-MB-231, MDA-MB-435s and SK-BR-3, which express Her2/neu receptor in different extent. Average Raman spectra of the different cell populations show prominent lipid presence in all cell lines. With high significance, Raman difference spectra reveal increased lipid contents, as well as a lower degree of fatty acid saturation in the MDA-MB cell lines with respect to the SK-BR-3 cells. These results are confirmed by hierarchical cluster analysis of single cells. High internal consistency of the chemical compositions in the cell lines is shown by hierarchical cluster analysis on a single matrix composed of the data of different cells from a single cell line. Although Her2/neu expression is highest for SK-BR-3 cells, their lipid contents are lower than that of the MDA-MB cell lines, which express less to no Her2/neu receptors. Rather than metabolic rate or senescence, the degree of metastaticity of the cells appears to be related to the polyunsaturated fatty acid contents of the cells.
U2 - 10.1039/C0AN00524J
DO - 10.1039/C0AN00524J
M3 - Article
SN - 0003-2654
VL - 135
SP - 3220
EP - 3226
JO - Analyst
JF - Analyst
IS - 12
ER -