A facile method to fabricate poly(L-lactide) nano-fibrous morphologies by phase inversion

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)

Abstract

Scaffolds with a nano-fibrous morphology are favored for certain tissue engineering applications as this morphology mimics the tissue’s natural extracellular matrix secreted by the cells, which consists of mainly collagen fibers with diameters ranging from 50 to 400 nm. Porous poly(l-lactide) (PLLA) scaffolds obtained by phase inversion methods generally have a solid-wall pore morphology. In contrast, this work presents a facile method to fabricate highly porous and highly interconnected nano-fibrous scaffold sheets by phase inversion using PLLA of very high molecular weight (5.7 × 105 g mol–1). The scaffold sheets consist of nano-fibers within the desired range of 50–500 nm. When applying phase separation micromolding as a fabrication method besides the porous nano-fibrous morphology, an additional topography can be introduced into these sheets. Culturing of C2C12 pre-myoblasts on these nano-fibrous sheets reveals very good cell adhesion, morphology and proliferation. Excellent alignment of the cells is induced by fabrication of 25 μm wide microchannels in these sheets. These results warrant further evaluation of these sheets as tissue engineering scaffolds
Original languageEnglish
Pages (from-to)2477-2483
JournalActa biomaterialia
Volume6
Issue number7
DOIs
Publication statusPublished - 2010

Keywords

  • METIS-266482
  • Porosity
  • Poly(L-lactic acid)
  • Phase separation
  • Nano-fiber
  • Molecular weight
  • IR-71492

Fingerprint Dive into the research topics of 'A facile method to fabricate poly(L-lactide) nano-fibrous morphologies by phase inversion'. Together they form a unique fingerprint.

Cite this