A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array

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Abstract

This paper reports a novel approach to build large arrays of cell-laden hydrogel microcompartments with well-controlled fluid flow to mimic the gut environment. Human intestinal epithelial cells (Caco-2) that were cultured in ~500 discontinuous compartments spontaneously grew into 3D folds on day 3. Mimicking interaction between intestinal eptithelial cells and intestinal bacteria was demonstrated in a long-term co-culture of E. coli adhered to Caco-2, the viability of which remained >70%. Also, different compartment geometries with large and small hydrogel interfaces were found to affect proliferation and cell spreading of Caco-2.
Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences
EditorsAbraham Lee, Don DeVoe
PublisherThe Chemical and Biological Microsystems Society
Pages1013
Number of pages2
ISBN (Print)978-0-692-94183-6
Publication statusPublished - 22 Oct 2017
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017 - Savannah International Trade & Convention Center, Savannah, United States
Duration: 22 Oct 201726 Oct 2017
Conference number: 21
https://www.microtas2017.org/

Publication series

NameInternational Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)
Volume2017
ISSN (Print)1556-5904

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017
Abbreviated titleMicroTAS
CountryUnited States
CitySavannah
Period22/10/1726/10/17
Internet address

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Keywords

  • Gut-on-chip
  • Microfluidics
  • 3D culture array

Cite this

Gümüscü, B., Albers, H. J., van den Berg, A., Eijkel, J., & van der Meer, A. D. (2017). A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array. In A. Lee, & D. DeVoe (Eds.), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1013). (International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS); Vol. 2017). The Chemical and Biological Microsystems Society.