A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

This paper reports a novel approach to build large arrays of cell-laden hydrogel microcompartments with well-controlled fluid flow to mimic the gut environment. Human intestinal epithelial cells (Caco-2) that were cultured in ~500 discontinuous compartments spontaneously grew into 3D folds on day 3. Mimicking interaction between intestinal eptithelial cells and intestinal bacteria was demonstrated in a long-term co-culture of E. coli adhered to Caco-2, the viability of which remained >70%. Also, different compartment geometries with large and small hydrogel interfaces were found to affect proliferation and cell spreading of Caco-2.
Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences
EditorsAbraham Lee, Don DeVoe
PublisherThe Chemical and Biological Microsystems Society
Pages1013
Number of pages2
ISBN (Print)978-0-692-94183-6
Publication statusPublished - 22 Oct 2017
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017 - Savannah International Trade & Convention Center, Savannah, United States
Duration: 22 Oct 201726 Oct 2017
Conference number: 21
https://www.microtas2017.org/

Publication series

Name
ISSN (Print)1556-5904

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017
Abbreviated titleMicroTAS
CountryUnited States
CitySavannah
Period22/10/1726/10/17
Internet address

Fingerprint

Cellular Microenvironment
Hydrogel
Caco-2 Cells
Coculture Techniques
Epithelial Cells
Escherichia coli
Bacteria

Cite this

Gümüscü Sefünc, B., Albers, H. J., van den Berg, A., Eijkel, J. C. T., & van der Meer, A. (2017). A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array. In A. Lee, & D. DeVoe (Eds.), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1013). The Chemical and Biological Microsystems Society.
Gümüscü Sefünc, Burcu ; Albers, Hugo Johan ; van den Berg, Albert ; Eijkel, Jan C.T. ; van der Meer, A. / A gut-on-a-chip study : Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. editor / Abraham Lee ; Don DeVoe. The Chemical and Biological Microsystems Society, 2017. pp. 1013
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title = "A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array",
abstract = "This paper reports a novel approach to build large arrays of cell-laden hydrogel microcompartments with well-controlled fluid flow to mimic the gut environment. Human intestinal epithelial cells (Caco-2) that were cultured in ~500 discontinuous compartments spontaneously grew into 3D folds on day 3. Mimicking interaction between intestinal eptithelial cells and intestinal bacteria was demonstrated in a long-term co-culture of E. coli adhered to Caco-2, the viability of which remained >70{\%}. Also, different compartment geometries with large and small hydrogel interfaces were found to affect proliferation and cell spreading of Caco-2.",
author = "{G{\"u}m{\"u}sc{\"u} Sef{\"u}nc}, Burcu and Albers, {Hugo Johan} and {van den Berg}, Albert and Eijkel, {Jan C.T.} and {van der Meer}, A.",
year = "2017",
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Gümüscü Sefünc, B, Albers, HJ, van den Berg, A, Eijkel, JCT & van der Meer, A 2017, A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array. in A Lee & D DeVoe (eds), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society, pp. 1013, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017, Savannah, United States, 22/10/17.

A gut-on-a-chip study : Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array. / Gümüscü Sefünc, Burcu ; Albers, Hugo Johan; van den Berg, Albert ; Eijkel, Jan C.T.; van der Meer, A.

21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. ed. / Abraham Lee; Don DeVoe. The Chemical and Biological Microsystems Society, 2017. p. 1013.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - A gut-on-a-chip study

T2 - Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array

AU - Gümüscü Sefünc, Burcu

AU - Albers, Hugo Johan

AU - van den Berg, Albert

AU - Eijkel, Jan C.T.

AU - van der Meer, A.

PY - 2017/10/22

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N2 - This paper reports a novel approach to build large arrays of cell-laden hydrogel microcompartments with well-controlled fluid flow to mimic the gut environment. Human intestinal epithelial cells (Caco-2) that were cultured in ~500 discontinuous compartments spontaneously grew into 3D folds on day 3. Mimicking interaction between intestinal eptithelial cells and intestinal bacteria was demonstrated in a long-term co-culture of E. coli adhered to Caco-2, the viability of which remained >70%. Also, different compartment geometries with large and small hydrogel interfaces were found to affect proliferation and cell spreading of Caco-2.

AB - This paper reports a novel approach to build large arrays of cell-laden hydrogel microcompartments with well-controlled fluid flow to mimic the gut environment. Human intestinal epithelial cells (Caco-2) that were cultured in ~500 discontinuous compartments spontaneously grew into 3D folds on day 3. Mimicking interaction between intestinal eptithelial cells and intestinal bacteria was demonstrated in a long-term co-culture of E. coli adhered to Caco-2, the viability of which remained >70%. Also, different compartment geometries with large and small hydrogel interfaces were found to affect proliferation and cell spreading of Caco-2.

M3 - Conference contribution

SN - 978-0-692-94183-6

SP - 1013

BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences

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A2 - DeVoe, Don

PB - The Chemical and Biological Microsystems Society

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Gümüscü Sefünc B, Albers HJ, van den Berg A, Eijkel JCT, van der Meer A. A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array. In Lee A, DeVoe D, editors, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society. 2017. p. 1013