A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array

Burcu  Gümüscü; Hugo J. Albers; Albert  van den Berg; Jan Eijkel; Andries D. van der Meer

A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array

Burcu Gümüscü, Hugo J. Albers, Albert van den Berg, Jan Eijkel, Andries D. van der Meer

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

This paper reports a novel approach to build large arrays of cell-laden hydrogel microcompartments with well-controlled fluid flow to mimic the gut environment. Human intestinal epithelial cells (Caco-2) that were cultured in ~500 discontinuous compartments spontaneously grew into 3D folds on day 3. Mimicking interaction between intestinal eptithelial cells and intestinal bacteria was demonstrated in a long-term co-culture of E. coli adhered to Caco-2, the viability of which remained >70%. Also, different compartment geometries with large and small hydrogel interfaces were found to affect proliferation and cell spreading of Caco-2.

Original language	English
Title of host publication	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences
Editors	Abraham Lee, Don DeVoe
Publisher	The Chemical and Biological Microsystems Society
Pages	1013
Number of pages	2
ISBN (Print)	978-0-692-94183-6
Publication status	Published - 22 Oct 2017
Event	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017 - Savannah International Trade & Convention Center, Savannah, United States Duration: 22 Oct 2017 → 26 Oct 2017 Conference number: 21 https://www.microtas2017.org/

Publication series

Name	International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)
Volume	2017
ISSN (Print)	1556-5904

Conference

Conference	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017
Abbreviated title	MicroTAS
Country/Territory	United States
City	Savannah
Period	22/10/17 → 26/10/17
Internet address	https://www.microtas2017.org/

Keywords

Gut-on-chip
Microfluidics
3D culture array

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Gümüscü, B., Albers, H. J., van den Berg, A., Eijkel, J., & van der Meer, A. D. (2017). A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array. In A. Lee, & D. DeVoe (Eds.), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1013). (International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS); Vol. 2017). The Chemical and Biological Microsystems Society.

Gümüscü, Burcu ; Albers, Hugo J. ; van den Berg, Albert et al. / A gut-on-a-chip study : Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. editor / Abraham Lee ; Don DeVoe. The Chemical and Biological Microsystems Society, 2017. pp. 1013 (International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)).

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title = "A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array",

abstract = "This paper reports a novel approach to build large arrays of cell-laden hydrogel microcompartments with well-controlled fluid flow to mimic the gut environment. Human intestinal epithelial cells (Caco-2) that were cultured in ~500 discontinuous compartments spontaneously grew into 3D folds on day 3. Mimicking interaction between intestinal eptithelial cells and intestinal bacteria was demonstrated in a long-term co-culture of E. coli adhered to Caco-2, the viability of which remained >70%. Also, different compartment geometries with large and small hydrogel interfaces were found to affect proliferation and cell spreading of Caco-2.",

keywords = "Gut-on-chip, Microfluidics, 3D culture array",

author = "Burcu G{\"u}m{\"u}sc{\"u} and Albers, {Hugo J.} and {van den Berg}, Albert and Jan Eijkel and {van der Meer}, {Andries D.}",

year = "2017",

month = oct,

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isbn = "978-0-692-94183-6",

series = "International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)",

publisher = "The Chemical and Biological Microsystems Society",

pages = "1013",

editor = "Abraham Lee and Don DeVoe",

booktitle = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences",

note = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017, MicroTAS ; Conference date: 22-10-2017 Through 26-10-2017",

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Gümüscü, B, Albers, HJ, van den Berg, A , Eijkel, J & van der Meer, AD 2017, A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array. in A Lee & D DeVoe (eds), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS), vol. 2017, The Chemical and Biological Microsystems Society, pp. 1013, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017, Savannah, Georgia, United States, 22/10/17.

A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array. / Gümüscü, Burcu ; Albers, Hugo J.; van den Berg, Albert et al.
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. ed. / Abraham Lee; Don DeVoe. The Chemical and Biological Microsystems Society, 2017. p. 1013 (International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS); Vol. 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - A gut-on-a-chip study

T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017

AU - Gümüscü, Burcu

AU - Albers, Hugo J.

AU - van den Berg, Albert

AU - Eijkel, Jan

AU - van der Meer, Andries D.

N1 - Conference code: 21

PY - 2017/10/22

Y1 - 2017/10/22

N2 - This paper reports a novel approach to build large arrays of cell-laden hydrogel microcompartments with well-controlled fluid flow to mimic the gut environment. Human intestinal epithelial cells (Caco-2) that were cultured in ~500 discontinuous compartments spontaneously grew into 3D folds on day 3. Mimicking interaction between intestinal eptithelial cells and intestinal bacteria was demonstrated in a long-term co-culture of E. coli adhered to Caco-2, the viability of which remained >70%. Also, different compartment geometries with large and small hydrogel interfaces were found to affect proliferation and cell spreading of Caco-2.

AB - This paper reports a novel approach to build large arrays of cell-laden hydrogel microcompartments with well-controlled fluid flow to mimic the gut environment. Human intestinal epithelial cells (Caco-2) that were cultured in ~500 discontinuous compartments spontaneously grew into 3D folds on day 3. Mimicking interaction between intestinal eptithelial cells and intestinal bacteria was demonstrated in a long-term co-culture of E. coli adhered to Caco-2, the viability of which remained >70%. Also, different compartment geometries with large and small hydrogel interfaces were found to affect proliferation and cell spreading of Caco-2.

KW - Gut-on-chip

KW - Microfluidics

KW - 3D culture array

M3 - Conference contribution

SN - 978-0-692-94183-6

T3 - International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)

SP - 1013

BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences

A2 - Lee, Abraham

A2 - DeVoe, Don

PB - The Chemical and Biological Microsystems Society

Y2 - 22 October 2017 through 26 October 2017

ER -

Gümüscü B, Albers HJ, van den Berg A , Eijkel J , van der Meer AD. A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array. In Lee A, DeVoe D, editors, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society. 2017. p. 1013. (International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)).

A gut-on-a-chip study: Enabling on-demand manipulation of the outer cell microenvironment in a multicompartmental 3D culture array

Abstract

Publication series

Conference

Keywords

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