A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells

Ravi Sinha; Severine le Gac; Nicolaas Jacobus Joseph Verdonschot; Albert van den Berg; Hubertus F.J.M. Koopman; Jeroen Rouwkema

doi:10.1039/c4lc01259c

A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells

Ravi Sinha, Severine le Gac, Nicolaas Jacobus Joseph Verdonschot, Albert van den Berg, Hubertus F.J.M. Koopman, Jeroen Rouwkema

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

We report a medium throughput device to study the effects of combinations of two mechanical stimuli – surface strains and fluid flow shear stresses, on cells. The first generation prototype can screen combinations of five strain and five shear stress levels. Computational modeling and empirical measurements were used to determine the generated strains and flows. Uniform equibiaxial strains up to 20% and shear stresses up to 0.3 Pa can be generated. Compatibility of the device with cell culture and end point fixation, staining and imaging is shown using C2C12 mouse myoblast cells.

Original language	English
Pages (from-to)	429-439
Number of pages	11
Journal	Lab on a chip
Volume	15
Issue number	2
DOIs	https://doi.org/10.1039/c4lc01259c
Publication status	Published - 31 Oct 2014

Keywords

2024 OA procedure

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10.1039/c4lc01259c

c4lc01259cFinal published version, 5.43 MBLicence: Taverne

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AU - Sinha, Ravi

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AU - van den Berg, Albert

AU - Koopman, Hubertus F.J.M.

AU - Rouwkema, Jeroen

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AB - We report a medium throughput device to study the effects of combinations of two mechanical stimuli – surface strains and fluid flow shear stresses, on cells. The first generation prototype can screen combinations of five strain and five shear stress levels. Computational modeling and empirical measurements were used to determine the generated strains and flows. Uniform equibiaxial strains up to 20% and shear stresses up to 0.3 Pa can be generated. Compatibility of the device with cell culture and end point fixation, staining and imaging is shown using C2C12 mouse myoblast cells.

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A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells

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Keywords

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