A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells

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Abstract

We report a medium throughput device to study the effects of combinations of two mechanical stimuli – surface strains and fluid flow shear stresses, on cells. The first generation prototype can screen combinations of five strain and five shear stress levels. Computational modeling and empirical measurements were used to determine the generated strains and flows. Uniform equibiaxial strains up to 20% and shear stresses up to 0.3 Pa can be generated. Compatibility of the device with cell culture and end point fixation, staining and imaging is shown using C2C12 mouse myoblast cells.
Original languageEnglish
Pages (from-to)429-439
Number of pages11
JournalLab on a chip
Volume15
Issue number2
DOIs
Publication statusPublished - 31 Oct 2015

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Shear stress
Flow of fluids
Throughput
Equipment and Supplies
Myoblasts
Cell Culture Techniques
Staining and Labeling
Cell culture
Imaging techniques

Keywords

  • IR-94283
  • METIS-308707
  • EWI-26773

Cite this

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title = "A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells",
abstract = "We report a medium throughput device to study the effects of combinations of two mechanical stimuli – surface strains and fluid flow shear stresses, on cells. The first generation prototype can screen combinations of five strain and five shear stress levels. Computational modeling and empirical measurements were used to determine the generated strains and flows. Uniform equibiaxial strains up to 20{\%} and shear stresses up to 0.3 Pa can be generated. Compatibility of the device with cell culture and end point fixation, staining and imaging is shown using C2C12 mouse myoblast cells.",
keywords = "IR-94283, METIS-308707, EWI-26773",
author = "Ravi Sinha and {le Gac}, Severine and Verdonschot, {Nicolaas Jacobus Joseph} and {van den Berg}, Albert and Koopman, {Hubertus F.J.M.} and Jeroen Rouwkema",
year = "2015",
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T1 - A medium throughput device to study the effects of combinations of surface strains and fluid-flow shear stresses on cells

AU - Sinha, Ravi

AU - le Gac, Severine

AU - Verdonschot, Nicolaas Jacobus Joseph

AU - van den Berg, Albert

AU - Koopman, Hubertus F.J.M.

AU - Rouwkema, Jeroen

PY - 2015/10/31

Y1 - 2015/10/31

N2 - We report a medium throughput device to study the effects of combinations of two mechanical stimuli – surface strains and fluid flow shear stresses, on cells. The first generation prototype can screen combinations of five strain and five shear stress levels. Computational modeling and empirical measurements were used to determine the generated strains and flows. Uniform equibiaxial strains up to 20% and shear stresses up to 0.3 Pa can be generated. Compatibility of the device with cell culture and end point fixation, staining and imaging is shown using C2C12 mouse myoblast cells.

AB - We report a medium throughput device to study the effects of combinations of two mechanical stimuli – surface strains and fluid flow shear stresses, on cells. The first generation prototype can screen combinations of five strain and five shear stress levels. Computational modeling and empirical measurements were used to determine the generated strains and flows. Uniform equibiaxial strains up to 20% and shear stresses up to 0.3 Pa can be generated. Compatibility of the device with cell culture and end point fixation, staining and imaging is shown using C2C12 mouse myoblast cells.

KW - IR-94283

KW - METIS-308707

KW - EWI-26773

U2 - 10.1039/c4lc01259c

DO - 10.1039/c4lc01259c

M3 - Article

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SP - 429

EP - 439

JO - Lab on a chip

JF - Lab on a chip

SN - 1473-0197

IS - 2

ER -