Abstract
| Original language | Undefined |
|---|---|
| Article number | 10.1039/b822962g |
| Pages (from-to) | 1687-1693 |
| Number of pages | 7 |
| Journal | Lab on a chip |
| Volume | 9 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2009 |
Keywords
- online electrochemistry
- cell
- chromatography/mass spectrometry
- cou
- ms
- redox reaction
- IR-68301
- Electrospray mass spectrometry
- Electrodes
- Oxidation
- ed onlin
- METIS-264114
- EWI-16441
Cite this
}
A microfluidic chip for electrochemical conversions in drug metabolism studies. / Odijk, Mathieu; Baumann, A.; Lohmann, W.; van den Brink, Floris Teunis Gerardus; Olthuis, Wouter; Karst, U.; van den Berg, Albert.
In: Lab on a chip, Vol. 9, No. 12, 10.1039/b822962g, 2009, p. 1687-1693.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - A microfluidic chip for electrochemical conversions in drug metabolism studies
AU - Odijk, Mathieu
AU - Baumann, A.
AU - Lohmann, W.
AU - van den Brink, Floris Teunis Gerardus
AU - Olthuis, Wouter
AU - Karst, U.
AU - van den Berg, Albert
N1 - 10.1039/b822962g
PY - 2009
Y1 - 2009
N2 - We have designed a microfluidic microreactor chip for electrochemical conversion of analytes, containing a palladium reference electrode and platinum working and counter electrodes. The counter electrode is placed in a separate side-channel on chip to prevent unwanted side-products appearing in the measured spectrograms. Using this chip, cyclic voltammograms are measured in volumes of 9.6 nL. Furthermore the conversion efficiency of ferricyanide is characterized using UV/vis-spectroscopy. We have obtained an on-line conversion of 97% using a flow rate of 1 mu L/min. We have used the microreactor chip to study the electrochemical metabolism pathway of amodiaquine using electrochemistry (EC)-liquid chromatography (LC)-mass spectrometry (MS). We have compared our results with measurements obtained with commercially available electrochemical flow-through cells. Using our chip it was possible to obtain similar results. Therefore, we have fabricated an electrochemical cell on-chip which is used successfully in EC-UV/vis and EC-LC-MS experiments.
AB - We have designed a microfluidic microreactor chip for electrochemical conversion of analytes, containing a palladium reference electrode and platinum working and counter electrodes. The counter electrode is placed in a separate side-channel on chip to prevent unwanted side-products appearing in the measured spectrograms. Using this chip, cyclic voltammograms are measured in volumes of 9.6 nL. Furthermore the conversion efficiency of ferricyanide is characterized using UV/vis-spectroscopy. We have obtained an on-line conversion of 97% using a flow rate of 1 mu L/min. We have used the microreactor chip to study the electrochemical metabolism pathway of amodiaquine using electrochemistry (EC)-liquid chromatography (LC)-mass spectrometry (MS). We have compared our results with measurements obtained with commercially available electrochemical flow-through cells. Using our chip it was possible to obtain similar results. Therefore, we have fabricated an electrochemical cell on-chip which is used successfully in EC-UV/vis and EC-LC-MS experiments.
KW - online electrochemistry
KW - cell
KW - chromatography/mass spectrometry
KW - cou
KW - ms
KW - redox reaction
KW - IR-68301
KW - Electrospray mass spectrometry
KW - Electrodes
KW - Oxidation
KW - ed onlin
KW - METIS-264114
KW - EWI-16441
U2 - 10.1039/b822962g
DO - 10.1039/b822962g
M3 - Article
VL - 9
SP - 1687
EP - 1693
JO - Lab on a chip
JF - Lab on a chip
SN - 1473-0197
IS - 12
M1 - 10.1039/b822962g
ER -