A microfluidic chip for electrochemical conversions in drug metabolism studies

Mathieu Odijk, A. Baumann, W. Lohmann, Floris Teunis Gerardus van den Brink, Wouter Olthuis, U. Karst, Albert van den Berg

    Research output: Contribution to journalArticleAcademicpeer-review

    44 Citations (Scopus)

    Abstract

    We have designed a microfluidic microreactor chip for electrochemical conversion of analytes, containing a palladium reference electrode and platinum working and counter electrodes. The counter electrode is placed in a separate side-channel on chip to prevent unwanted side-products appearing in the measured spectrograms. Using this chip, cyclic voltammograms are measured in volumes of 9.6 nL. Furthermore the conversion efficiency of ferricyanide is characterized using UV/vis-spectroscopy. We have obtained an on-line conversion of 97% using a flow rate of 1 mu L/min. We have used the microreactor chip to study the electrochemical metabolism pathway of amodiaquine using electrochemistry (EC)-liquid chromatography (LC)-mass spectrometry (MS). We have compared our results with measurements obtained with commercially available electrochemical flow-through cells. Using our chip it was possible to obtain similar results. Therefore, we have fabricated an electrochemical cell on-chip which is used successfully in EC-UV/vis and EC-LC-MS experiments.
    Original languageUndefined
    Article number10.1039/b822962g
    Pages (from-to)1687-1693
    Number of pages7
    JournalLab on a chip
    Volume9
    Issue number12
    DOIs
    Publication statusPublished - 2009

    Keywords

    • online electrochemistry
    • cell
    • chromatography/mass spectrometry
    • cou
    • ms
    • redox reaction
    • IR-68301
    • Electrospray mass spectrometry
    • Electrodes
    • Oxidation
    • ed onlin
    • METIS-264114
    • EWI-16441

    Cite this

    Odijk, M., Baumann, A., Lohmann, W., van den Brink, F. T. G., Olthuis, W., Karst, U., & van den Berg, A. (2009). A microfluidic chip for electrochemical conversions in drug metabolism studies. Lab on a chip, 9(12), 1687-1693. [10.1039/b822962g]. https://doi.org/10.1039/b822962g
    Odijk, Mathieu ; Baumann, A. ; Lohmann, W. ; van den Brink, Floris Teunis Gerardus ; Olthuis, Wouter ; Karst, U. ; van den Berg, Albert. / A microfluidic chip for electrochemical conversions in drug metabolism studies. In: Lab on a chip. 2009 ; Vol. 9, No. 12. pp. 1687-1693.
    @article{93a90894317044b7a333134639dfb6a1,
    title = "A microfluidic chip for electrochemical conversions in drug metabolism studies",
    abstract = "We have designed a microfluidic microreactor chip for electrochemical conversion of analytes, containing a palladium reference electrode and platinum working and counter electrodes. The counter electrode is placed in a separate side-channel on chip to prevent unwanted side-products appearing in the measured spectrograms. Using this chip, cyclic voltammograms are measured in volumes of 9.6 nL. Furthermore the conversion efficiency of ferricyanide is characterized using UV/vis-spectroscopy. We have obtained an on-line conversion of 97{\%} using a flow rate of 1 mu L/min. We have used the microreactor chip to study the electrochemical metabolism pathway of amodiaquine using electrochemistry (EC)-liquid chromatography (LC)-mass spectrometry (MS). We have compared our results with measurements obtained with commercially available electrochemical flow-through cells. Using our chip it was possible to obtain similar results. Therefore, we have fabricated an electrochemical cell on-chip which is used successfully in EC-UV/vis and EC-LC-MS experiments.",
    keywords = "online electrochemistry, cell, chromatography/mass spectrometry, cou, ms, redox reaction, IR-68301, Electrospray mass spectrometry, Electrodes, Oxidation, ed onlin, METIS-264114, EWI-16441",
    author = "Mathieu Odijk and A. Baumann and W. Lohmann and {van den Brink}, {Floris Teunis Gerardus} and Wouter Olthuis and U. Karst and {van den Berg}, Albert",
    note = "10.1039/b822962g",
    year = "2009",
    doi = "10.1039/b822962g",
    language = "Undefined",
    volume = "9",
    pages = "1687--1693",
    journal = "Lab on a chip",
    issn = "1473-0197",
    publisher = "Royal Society of Chemistry",
    number = "12",

    }

    Odijk, M, Baumann, A, Lohmann, W, van den Brink, FTG, Olthuis, W, Karst, U & van den Berg, A 2009, 'A microfluidic chip for electrochemical conversions in drug metabolism studies', Lab on a chip, vol. 9, no. 12, 10.1039/b822962g, pp. 1687-1693. https://doi.org/10.1039/b822962g

    A microfluidic chip for electrochemical conversions in drug metabolism studies. / Odijk, Mathieu; Baumann, A.; Lohmann, W.; van den Brink, Floris Teunis Gerardus; Olthuis, Wouter; Karst, U.; van den Berg, Albert.

    In: Lab on a chip, Vol. 9, No. 12, 10.1039/b822962g, 2009, p. 1687-1693.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - A microfluidic chip for electrochemical conversions in drug metabolism studies

    AU - Odijk, Mathieu

    AU - Baumann, A.

    AU - Lohmann, W.

    AU - van den Brink, Floris Teunis Gerardus

    AU - Olthuis, Wouter

    AU - Karst, U.

    AU - van den Berg, Albert

    N1 - 10.1039/b822962g

    PY - 2009

    Y1 - 2009

    N2 - We have designed a microfluidic microreactor chip for electrochemical conversion of analytes, containing a palladium reference electrode and platinum working and counter electrodes. The counter electrode is placed in a separate side-channel on chip to prevent unwanted side-products appearing in the measured spectrograms. Using this chip, cyclic voltammograms are measured in volumes of 9.6 nL. Furthermore the conversion efficiency of ferricyanide is characterized using UV/vis-spectroscopy. We have obtained an on-line conversion of 97% using a flow rate of 1 mu L/min. We have used the microreactor chip to study the electrochemical metabolism pathway of amodiaquine using electrochemistry (EC)-liquid chromatography (LC)-mass spectrometry (MS). We have compared our results with measurements obtained with commercially available electrochemical flow-through cells. Using our chip it was possible to obtain similar results. Therefore, we have fabricated an electrochemical cell on-chip which is used successfully in EC-UV/vis and EC-LC-MS experiments.

    AB - We have designed a microfluidic microreactor chip for electrochemical conversion of analytes, containing a palladium reference electrode and platinum working and counter electrodes. The counter electrode is placed in a separate side-channel on chip to prevent unwanted side-products appearing in the measured spectrograms. Using this chip, cyclic voltammograms are measured in volumes of 9.6 nL. Furthermore the conversion efficiency of ferricyanide is characterized using UV/vis-spectroscopy. We have obtained an on-line conversion of 97% using a flow rate of 1 mu L/min. We have used the microreactor chip to study the electrochemical metabolism pathway of amodiaquine using electrochemistry (EC)-liquid chromatography (LC)-mass spectrometry (MS). We have compared our results with measurements obtained with commercially available electrochemical flow-through cells. Using our chip it was possible to obtain similar results. Therefore, we have fabricated an electrochemical cell on-chip which is used successfully in EC-UV/vis and EC-LC-MS experiments.

    KW - online electrochemistry

    KW - cell

    KW - chromatography/mass spectrometry

    KW - cou

    KW - ms

    KW - redox reaction

    KW - IR-68301

    KW - Electrospray mass spectrometry

    KW - Electrodes

    KW - Oxidation

    KW - ed onlin

    KW - METIS-264114

    KW - EWI-16441

    U2 - 10.1039/b822962g

    DO - 10.1039/b822962g

    M3 - Article

    VL - 9

    SP - 1687

    EP - 1693

    JO - Lab on a chip

    JF - Lab on a chip

    SN - 1473-0197

    IS - 12

    M1 - 10.1039/b822962g

    ER -

    Odijk M, Baumann A, Lohmann W, van den Brink FTG, Olthuis W, Karst U et al. A microfluidic chip for electrochemical conversions in drug metabolism studies. Lab on a chip. 2009;9(12):1687-1693. 10.1039/b822962g. https://doi.org/10.1039/b822962g