A microfluidic device for monitoring siRNA delivery under fluid now

Andries Dirk van der Meer, J. Feijen (Editor), Marloes Kamphuis, W.E. Hennink (Editor), Andreas A. Poot, A.P. Sam (Editor), Jan Feijen, I. Vermes

Research output: Contribution to conferencePaperAcademic

Abstract

When studying particle uptake in vitro, it is favorable to mimic the in vivo situation as much as possible. In this study we present a microfluidic device to mimic the mechanical stress caused by the flow of blood while studying particle uptake in vitro. Human endothelial cells were treated with liposomes containing fluorescent siRNA. It was found that applying physiologically relevant mechanical stress during transfection diminishes the uptake of liposomes in the cells.
Original languageUndefined
Pagese42-e44
DOIs
Publication statusPublished - 2 Apr 2008
Event10th European Symposium on Controlled Drug Delivery, ESCDD 2008 - Noordwijk aan Zee, Netherlands
Duration: 2 Apr 20084 Apr 2008
Conference number: 10

Conference

Conference10th European Symposium on Controlled Drug Delivery, ESCDD 2008
Abbreviated titleESCDD
CountryNetherlands
CityNoordwijk aan Zee
Period2/04/084/04/08

Keywords

  • IR-69301
  • METIS-254631

Cite this

van der Meer, A. D., Feijen, J. (Ed.), Kamphuis, M., Hennink, W. E. (Ed.), Poot, A. A., Sam, A. P. (Ed.), ... Vermes, I. (2008). A microfluidic device for monitoring siRNA delivery under fluid now. e42-e44. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands. https://doi.org/10.1016/j.jconrel.2008.09.028
van der Meer, Andries Dirk ; Feijen, J. (Editor) ; Kamphuis, Marloes ; Hennink, W.E. (Editor) ; Poot, Andreas A. ; Sam, A.P. (Editor) ; Feijen, Jan ; Vermes, I. / A microfluidic device for monitoring siRNA delivery under fluid now. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands.
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title = "A microfluidic device for monitoring siRNA delivery under fluid now",
abstract = "When studying particle uptake in vitro, it is favorable to mimic the in vivo situation as much as possible. In this study we present a microfluidic device to mimic the mechanical stress caused by the flow of blood while studying particle uptake in vitro. Human endothelial cells were treated with liposomes containing fluorescent siRNA. It was found that applying physiologically relevant mechanical stress during transfection diminishes the uptake of liposomes in the cells.",
keywords = "IR-69301, METIS-254631",
author = "{van der Meer}, {Andries Dirk} and J. Feijen and Marloes Kamphuis and W.E. Hennink and Poot, {Andreas A.} and A.P. Sam and Jan Feijen and I. Vermes",
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van der Meer, AD, Feijen, J (ed.), Kamphuis, M, Hennink, WE (ed.), Poot, AA, Sam, AP (ed.), Feijen, J & Vermes, I 2008, 'A microfluidic device for monitoring siRNA delivery under fluid now' Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands, 2/04/08 - 4/04/08, pp. e42-e44. https://doi.org/10.1016/j.jconrel.2008.09.028

A microfluidic device for monitoring siRNA delivery under fluid now. / van der Meer, Andries Dirk; Feijen, J. (Editor); Kamphuis, Marloes; Hennink, W.E. (Editor); Poot, Andreas A.; Sam, A.P. (Editor); Feijen, Jan; Vermes, I.

2008. e42-e44 Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands.

Research output: Contribution to conferencePaperAcademic

TY - CONF

T1 - A microfluidic device for monitoring siRNA delivery under fluid now

AU - van der Meer, Andries Dirk

AU - Kamphuis, Marloes

AU - Poot, Andreas A.

AU - Feijen, Jan

AU - Vermes, I.

A2 - Feijen, J.

A2 - Hennink, W.E.

A2 - Sam, A.P.

PY - 2008/4/2

Y1 - 2008/4/2

N2 - When studying particle uptake in vitro, it is favorable to mimic the in vivo situation as much as possible. In this study we present a microfluidic device to mimic the mechanical stress caused by the flow of blood while studying particle uptake in vitro. Human endothelial cells were treated with liposomes containing fluorescent siRNA. It was found that applying physiologically relevant mechanical stress during transfection diminishes the uptake of liposomes in the cells.

AB - When studying particle uptake in vitro, it is favorable to mimic the in vivo situation as much as possible. In this study we present a microfluidic device to mimic the mechanical stress caused by the flow of blood while studying particle uptake in vitro. Human endothelial cells were treated with liposomes containing fluorescent siRNA. It was found that applying physiologically relevant mechanical stress during transfection diminishes the uptake of liposomes in the cells.

KW - IR-69301

KW - METIS-254631

U2 - 10.1016/j.jconrel.2008.09.028

DO - 10.1016/j.jconrel.2008.09.028

M3 - Paper

SP - e42-e44

ER -

van der Meer AD, Feijen J, (ed.), Kamphuis M, Hennink WE, (ed.), Poot AA, Sam AP, (ed.) et al. A microfluidic device for monitoring siRNA delivery under fluid now. 2008. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands. https://doi.org/10.1016/j.jconrel.2008.09.028