A microfluidic device for monitoring siRNA delivery under fluid now

Andries Dirk van der Meer; J. Feijen; Marloes Kamphuis; W.E. Hennink; Andreas A. Poot; A.P. Sam; Jan Feijen; I. Vermes

doi:10.1016/j.jconrel.2008.09.028

A microfluidic device for monitoring siRNA delivery under fluid now

Andries Dirk van der Meer, J. Feijen (Editor), Marloes Kamphuis, W.E. Hennink (Editor), Andreas A. Poot, A.P. Sam (Editor), Jan Feijen, I. Vermes

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Abstract

When studying particle uptake in vitro, it is favorable to mimic the in vivo situation as much as possible. In this study we present a microfluidic device to mimic the mechanical stress caused by the flow of blood while studying particle uptake in vitro. Human endothelial cells were treated with liposomes containing fluorescent siRNA. It was found that applying physiologically relevant mechanical stress during transfection diminishes the uptake of liposomes in the cells.

Original language	Undefined
Pages	e42-e44
DOIs	https://doi.org/10.1016/j.jconrel.2008.09.028
Publication status	Published - 2 Apr 2008
Event	10th European Symposium on Controlled Drug Delivery, ESCDD 2008 - Noordwijk aan Zee, Netherlands Duration: 2 Apr 2008 → 4 Apr 2008 Conference number: 10

Conference

Conference	10th European Symposium on Controlled Drug Delivery, ESCDD 2008
Abbreviated title	ESCDD
Country/Territory	Netherlands
City	Noordwijk aan Zee
Period	2/04/08 → 4/04/08

Keywords

IR-69301
METIS-254631

Access to Document

10.1016/j.jconrel.2008.09.028

Cite this

van der Meer, A. D., Feijen, J. (Ed.), Kamphuis, M., Hennink, W. E. (Ed.), Poot, A. A., Sam, A. P. (Ed.), Feijen, J., & Vermes, I. (2008). A microfluidic device for monitoring siRNA delivery under fluid now. e42-e44. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands. https://doi.org/10.1016/j.jconrel.2008.09.028

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keywords = "IR-69301, METIS-254631",

author = "{van der Meer}, {Andries Dirk} and J. Feijen and Marloes Kamphuis and W.E. Hennink and Poot, {Andreas A.} and A.P. Sam and Jan Feijen and I. Vermes",

year = "2008",

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doi = "10.1016/j.jconrel.2008.09.028",

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van der Meer, AD, Feijen, J (ed.), Kamphuis, M, Hennink, WE (ed.), Poot, AA, Sam, AP (ed.), Feijen, J & Vermes, I 2008, 'A microfluidic device for monitoring siRNA delivery under fluid now', Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands, 2/04/08 - 4/04/08 pp. e42-e44. https://doi.org/10.1016/j.jconrel.2008.09.028

TY - CONF

T1 - A microfluidic device for monitoring siRNA delivery under fluid now

AU - van der Meer, Andries Dirk

AU - Kamphuis, Marloes

AU - Poot, Andreas A.

AU - Feijen, Jan

AU - Vermes, I.

A2 - Feijen, J.

A2 - Hennink, W.E.

A2 - Sam, A.P.

N1 - Conference code: 10

PY - 2008/4/2

Y1 - 2008/4/2

N2 - When studying particle uptake in vitro, it is favorable to mimic the in vivo situation as much as possible. In this study we present a microfluidic device to mimic the mechanical stress caused by the flow of blood while studying particle uptake in vitro. Human endothelial cells were treated with liposomes containing fluorescent siRNA. It was found that applying physiologically relevant mechanical stress during transfection diminishes the uptake of liposomes in the cells.

AB - When studying particle uptake in vitro, it is favorable to mimic the in vivo situation as much as possible. In this study we present a microfluidic device to mimic the mechanical stress caused by the flow of blood while studying particle uptake in vitro. Human endothelial cells were treated with liposomes containing fluorescent siRNA. It was found that applying physiologically relevant mechanical stress during transfection diminishes the uptake of liposomes in the cells.

KW - IR-69301

KW - METIS-254631

U2 - 10.1016/j.jconrel.2008.09.028

DO - 10.1016/j.jconrel.2008.09.028

M3 - Paper

SP - e42-e44

T2 - 10th European Symposium on Controlled Drug Delivery, ESCDD 2008

Y2 - 2 April 2008 through 4 April 2008

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