When studying particle uptake in vitro, it is favorable to mimic the in vivo situation as much as possible. In this study we present a microfluidic device to mimic the mechanical stress caused by the flow of blood while studying particle uptake in vitro. Human endothelial cells were treated with liposomes containing fluorescent siRNA. It was found that applying physiologically relevant mechanical stress during transfection diminishes the uptake of liposomes in the cells.
|Publication status||Published - 2 Apr 2008|
|Event||10th European Symposium on Controlled Drug Delivery, ESCDD 2008 - Noordwijk aan Zee, Netherlands|
Duration: 2 Apr 2008 → 4 Apr 2008
Conference number: 10
|Conference||10th European Symposium on Controlled Drug Delivery, ESCDD 2008|
|City||Noordwijk aan Zee|
|Period||2/04/08 → 4/04/08|