A microfluidic droplet serial dilutor with enhanced mixing

Hoon Suk Rho, Yoonsun Yang, Leon W.M.M. Terstappen, Pamela Habibović, Séverine le Gac

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Abstract

We present the development of an automated microfluidic platform that is capable of creating logarithmic serial dilution by adapting pneumatically actuated microvalves. A valve-assisted droplet manipulator integrated with a peristaltic mixer allowed the accurate formation of droplets with controlled sizes and the dilution of reagent sequentially. Hence, serial dilution with flexible dilution factors was obtained in a series of nanoliter-scale droplets. We validated the mixing efficiency and droplet generating performance of the chip at various operating conditions and demonstrated an example of logarithmic serial dilution with two different dilution factors. The microfluidic droplet serial dilutor could be used as an analytical tool to evaluate various complex chemical and biochemical reactions.
Original languageEnglish
Title of host publicationMicroTas 2018
Subtitle of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences
EditorsFan-Gang Tseng, Gwo-Bin Lee
PublisherThe Chemical and Biological Microsystems Society
Pages1114-1115
Number of pages2
ISBN (Electronic)978-1-510-89757-1
ISBN (Print)978-0-578-40530-8
Publication statusPublished - 11 Nov 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, Province of China
Duration: 10 Nov 201815 Nov 2018
Conference number: 22
https://cbmsociety.org/conferences/microtas2018/

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018
Abbreviated titleMicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period10/11/1815/11/18
Internet address

Keywords

  • Microfluidics
  • Droplets
  • Serial dilution
  • Microvalve

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  • Cite this

    Rho, H. S., Yang, Y., Terstappen, L. W. M. M., Habibović, P., & le Gac, S. (2018). A microfluidic droplet serial dilutor with enhanced mixing. In F-G. Tseng, & G-B. Lee (Eds.), MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1114-1115). The Chemical and Biological Microsystems Society.