We present the development of an automated microfluidic platform that is capable of creating logarithmic serial dilution by adapting pneumatically actuated microvalves. A valve-assisted droplet manipulator integrated with a peristaltic mixer allowed the accurate formation of droplets with controlled sizes and the dilution of reagent sequentially. Hence, serial dilution with flexible dilution factors was obtained in a series of nanoliter-scale droplets. We validated the mixing efficiency and droplet generating performance of the chip at various operating conditions and demonstrated an example of logarithmic serial dilution with two different dilution factors. The microfluidic droplet serial dilutor could be used as an analytical tool to evaluate various complex chemical and biochemical reactions.
|Title of host publication||MicroTas 2018|
|Subtitle of host publication||22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences|
|Editors||Fan-Gang Tseng, Gwo-Bin Lee|
|Publisher||The Chemical and Biological Microsystems Society|
|Number of pages||2|
|Publication status||Published - 11 Nov 2018|
|Event||22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, Province of China|
Duration: 10 Nov 2018 → 15 Nov 2018
Conference number: 22
|Conference||22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2018|
|Abbreviated title||MicroTAS 2018|
|Country||Taiwan, Province of China|
|Period||10/11/18 → 15/11/18|
- Serial dilution
Rho, H. S., Yang, Y., Terstappen, L. W. M. M., Habibović, P., & le Gac, S. (2018). A microfluidic droplet serial dilutor with enhanced mixing. In F-G. Tseng, & G-B. Lee (Eds.), MicroTas 2018: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1114-1115). The Chemical and Biological Microsystems Society.