A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain

Floris T.G. Van Den Brink, Thas Phisonkunkasem, Ashish Asthana, Johan G. Bomer, Arn M.J.M. Van Den Maagdenberg, Else A. Tolner, Mathieu Odijk*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    2 Citations (Scopus)
    9 Downloads (Pure)

    Abstract

    Measuring biomolecule concentrations in the brain of living animals, in real time, is a challenging task, especially when detailed information at high temporal resolution is also required. Traditionally, microdialysis probes are used that generally have sampling areas in the order of about 1 mm 2 , and provide information on concentrations with a temporal resolution of at least several minutes. In this paper, we present a novel miniaturized push-pull perfusion sampling probe that uses an array of small 3 μm-wide sampling channels to sample neurotransmitters at a typical recovery rate of 61%, with a reduced risk of clogging. The added feature to segment the dialysate inside the probe into small water-in-decane droplets enables the detection of concentrations with a temporal resolution of a few seconds. Here we used the probe for in vivo recordings of neurotransmitter glutamate released upon electrical stimulation in the brain of a mouse to demonstrate the feasibility of the probe for real-time neurochemical brain analysis.

    Original languageEnglish
    Pages (from-to)1332-1343
    Number of pages12
    JournalLab on a chip
    Volume19
    Issue number8
    DOIs
    Publication statusE-pub ahead of print/First online - 5 Mar 2019

    Fingerprint

    Neurotransmitter Agents
    Brain
    Perfusion
    Sampling
    Deep Brain Stimulation
    Dialysis Solutions
    Microdialysis
    Biomolecules
    Glutamic Acid
    Animals
    Recovery
    Water

    Cite this

    Van Den Brink, F. T. G., Phisonkunkasem, T., Asthana, A., Bomer, J. G., Van Den Maagdenberg, A. M. J. M., Tolner, E. A., & Odijk, M. (2019). A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain. Lab on a chip, 19(8), 1332-1343. https://doi.org/10.1039/c8lc01137k
    Van Den Brink, Floris T.G. ; Phisonkunkasem, Thas ; Asthana, Ashish ; Bomer, Johan G. ; Van Den Maagdenberg, Arn M.J.M. ; Tolner, Else A. ; Odijk, Mathieu. / A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain. In: Lab on a chip. 2019 ; Vol. 19, No. 8. pp. 1332-1343.
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    abstract = "Measuring biomolecule concentrations in the brain of living animals, in real time, is a challenging task, especially when detailed information at high temporal resolution is also required. Traditionally, microdialysis probes are used that generally have sampling areas in the order of about 1 mm 2 , and provide information on concentrations with a temporal resolution of at least several minutes. In this paper, we present a novel miniaturized push-pull perfusion sampling probe that uses an array of small 3 μm-wide sampling channels to sample neurotransmitters at a typical recovery rate of 61{\%}, with a reduced risk of clogging. The added feature to segment the dialysate inside the probe into small water-in-decane droplets enables the detection of concentrations with a temporal resolution of a few seconds. Here we used the probe for in vivo recordings of neurotransmitter glutamate released upon electrical stimulation in the brain of a mouse to demonstrate the feasibility of the probe for real-time neurochemical brain analysis.",
    author = "{Van Den Brink}, {Floris T.G.} and Thas Phisonkunkasem and Ashish Asthana and Bomer, {Johan G.} and {Van Den Maagdenberg}, {Arn M.J.M.} and Tolner, {Else A.} and Mathieu Odijk",
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    Van Den Brink, FTG, Phisonkunkasem, T, Asthana, A, Bomer, JG, Van Den Maagdenberg, AMJM, Tolner, EA & Odijk, M 2019, 'A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain', Lab on a chip, vol. 19, no. 8, pp. 1332-1343. https://doi.org/10.1039/c8lc01137k

    A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain. / Van Den Brink, Floris T.G.; Phisonkunkasem, Thas; Asthana, Ashish; Bomer, Johan G.; Van Den Maagdenberg, Arn M.J.M.; Tolner, Else A.; Odijk, Mathieu.

    In: Lab on a chip, Vol. 19, No. 8, 05.03.2019, p. 1332-1343.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - Van Den Brink, Floris T.G.

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    AU - Asthana, Ashish

    AU - Bomer, Johan G.

    AU - Van Den Maagdenberg, Arn M.J.M.

    AU - Tolner, Else A.

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    Van Den Brink FTG, Phisonkunkasem T, Asthana A, Bomer JG, Van Den Maagdenberg AMJM, Tolner EA et al. A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain. Lab on a chip. 2019 Mar 5;19(8):1332-1343. https://doi.org/10.1039/c8lc01137k