A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain

Floris T.G. Van Den Brink, Thas Phisonkunkasem, Ashish Asthana, Johan G. Bomer, Arn M.J.M. Van Den Maagdenberg, Else A. Tolner, Mathieu Odijk

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Abstract

Measuring biomolecule concentrations in the brain of living animals, in real time, is a challenging task, especially when detailed information at high temporal resolution is also required. Traditionally, microdialysis probes are used that generally have sampling areas in the order of about 1 mm 2 , and provide information on concentrations with a temporal resolution of at least several minutes. In this paper, we present a novel miniaturized push-pull perfusion sampling probe that uses an array of small 3 μm-wide sampling channels to sample neurotransmitters at a typical recovery rate of 61%, with a reduced risk of clogging. The added feature to segment the dialysate inside the probe into small water-in-decane droplets enables the detection of concentrations with a temporal resolution of a few seconds. Here we used the probe for in vivo recordings of neurotransmitter glutamate released upon electrical stimulation in the brain of a mouse to demonstrate the feasibility of the probe for real-time neurochemical brain analysis.

Original languageEnglish
Pages (from-to)1332-1343
Number of pages12
JournalLab on a chip
Volume19
Issue number8
DOIs
Publication statusE-pub ahead of print/First online - 5 Mar 2019

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Neurotransmitter Agents
Brain
Perfusion
Sampling
Deep Brain Stimulation
Dialysis Solutions
Microdialysis
Biomolecules
Glutamic Acid
Animals
Recovery
Water

Cite this

Van Den Brink, F. T. G., Phisonkunkasem, T., Asthana, A., Bomer, J. G., Van Den Maagdenberg, A. M. J. M., Tolner, E. A., & Odijk, M. (2019). A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain. Lab on a chip, 19(8), 1332-1343. https://doi.org/10.1039/c8lc01137k
Van Den Brink, Floris T.G. ; Phisonkunkasem, Thas ; Asthana, Ashish ; Bomer, Johan G. ; Van Den Maagdenberg, Arn M.J.M. ; Tolner, Else A. ; Odijk, Mathieu. / A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain. In: Lab on a chip. 2019 ; Vol. 19, No. 8. pp. 1332-1343.
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Van Den Brink, FTG, Phisonkunkasem, T, Asthana, A, Bomer, JG, Van Den Maagdenberg, AMJM, Tolner, EA & Odijk, M 2019, 'A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain' Lab on a chip, vol. 19, no. 8, pp. 1332-1343. https://doi.org/10.1039/c8lc01137k

A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain. / Van Den Brink, Floris T.G.; Phisonkunkasem, Thas; Asthana, Ashish; Bomer, Johan G.; Van Den Maagdenberg, Arn M.J.M.; Tolner, Else A.; Odijk, Mathieu.

In: Lab on a chip, Vol. 19, No. 8, 05.03.2019, p. 1332-1343.

Research output: Contribution to journalArticleAcademicpeer-review

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Van Den Brink FTG, Phisonkunkasem T, Asthana A, Bomer JG, Van Den Maagdenberg AMJM, Tolner EA et al. A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain. Lab on a chip. 2019 Mar 5;19(8):1332-1343. https://doi.org/10.1039/c8lc01137k