A miniaturized push-pull-perfusion probe for few-second sampling of neurotransmitters in the mouse brain

Floris T.G. Van Den Brink, Thas Phisonkunkasem, Ashish Asthana, Johan G. Bomer, Arn M.J.M. Van Den Maagdenberg, Else A. Tolner, Mathieu Odijk*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)
21 Downloads (Pure)

Abstract

Measuring biomolecule concentrations in the brain of living animals, in real time, is a challenging task, especially when detailed information at high temporal resolution is also required. Traditionally, microdialysis probes are used that generally have sampling areas in the order of about 1 mm 2 , and provide information on concentrations with a temporal resolution of at least several minutes. In this paper, we present a novel miniaturized push-pull perfusion sampling probe that uses an array of small 3 μm-wide sampling channels to sample neurotransmitters at a typical recovery rate of 61%, with a reduced risk of clogging. The added feature to segment the dialysate inside the probe into small water-in-decane droplets enables the detection of concentrations with a temporal resolution of a few seconds. Here we used the probe for in vivo recordings of neurotransmitter glutamate released upon electrical stimulation in the brain of a mouse to demonstrate the feasibility of the probe for real-time neurochemical brain analysis.

Original languageEnglish
Pages (from-to)1332-1343
Number of pages12
JournalLab on a chip
Volume19
Issue number8
Early online date5 Mar 2019
DOIs
Publication statusPublished - 21 Apr 2019

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