A modified algorithm for continuous wave near infrared spectroscopy applied to in-vivo animal experiments and on human skin

John H.G.M. Klaessens, Jeroen C.W. Hopman, K. Djien Liem, Rowland De Roode, Rudolf M. Verdaasdonk, Johan M. Thijssen

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

2 Citations (Scopus)
39 Downloads (Pure)


Continuous wave Near Infrared Spectroscopy is a well known non invasive technique for measuring changes in tissue oxygenation. Absorption changes (ΔO2Hb and ΔHHb) are calculated from the light attenuations using the modified Lambert Beer equation. Generally, the concentration changes are calculated relative to the concentration at a starting point in time (delta time method). It is also possible, under certain assumptions, to calculate the concentrations by subtracting the equations at different wavelengths (delta wavelength method). We derived a new algorithm and will show the possibilities and limitations. In the delta wavelength method, the assumption is that the oxygen independent attenuation term will be eliminated from the formula even if its value changes in time, we verified the results with the classical delta time method using extinction coefficients from different literature sources for the wavelengths 767nm, 850nm and 905nm. The different methods of calculating concentration changes were applied to the data collected from animal experiments. The animals (lambs) were in a stable normoxic condition; stepwise they were made hypoxic and thereafter they returned to normoxic condition. The two algorithms were also applied for measuring two dimensional blood oxygen saturation changes in human skin tissue. The different oxygen saturation levels were induced by alterations in the respiration and by temporary arm clamping. The new delta wavelength method yielded in a steady state measurement the same changes in oxy and deoxy hemoglobin as the classical delta time method. The advantage of the new method is the independence of eventual variation of the oxygen independent attenuations in time.

Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems VI
Publication statusPublished - 21 Apr 2008
Externally publishedYes
EventAdvanced Biomedical and Clinical Diagnostic Systems VI 2008 - San Jose, United States
Duration: 20 Jan 200821 Jan 2008
Conference number: 6

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceAdvanced Biomedical and Clinical Diagnostic Systems VI 2008
Country/TerritoryUnited States
CitySan Jose


  • 2D
  • Algorithm
  • Hemoglobin
  • Methods
  • Near infrared
  • Oxygen
  • Skin
  • Spectroscopy


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