A nanofluidic mixing device for high-throughput fluorescence sensing of single molecules

Klaus Mathwig; Carel Fijen; Mattia Fontana; Serge G. Lemay; Johannes Hohlbein

doi:10.1016/j.protcy.2017.04.062

A nanofluidic mixing device for high-throughput fluorescence sensing of single molecules

Klaus Mathwig, Carel Fijen, Mattia Fontana, Serge G. Lemay, Johannes Hohlbein

Bio electronics

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Abstract

We introduce a nanofluidic mixing device entirely fabricated in glass for the fluorescence detection of single molecules. The design consists of a nanochannel T-junction and allows the continuous monitoring of chemical or enzymatic reactions of analytes as they arrive from two independent inlets. The fluorescently labeled molecules are tracked before, during and after they enter the mixing region, and their reactions with each other are observed by means of optical readout such as Förster Resonance Energy Transfer (FRET). Our method can be used for analyzing the kinetics of DNA annealing in a high-parallelized fashion.

Original language	English
Pages (from-to)	141-142
Journal	Procedia technology
Volume	27
DOIs	https://doi.org/10.1016/j.protcy.2017.04.062
Publication status	Published - 2017

Keywords

nanochannel
nanofluidic mixing
fluorescence detection
single molecules

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10.1016/j.protcy.2017.04.062

1-s2.0-S2212017317300634-mainFinal published version, 1.33 MBLicence: CC BY-NC-ND

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abstract = "We introduce a nanofluidic mixing device entirely fabricated in glass for the fluorescence detection of single molecules. The design consists of a nanochannel T-junction and allows the continuous monitoring of chemical or enzymatic reactions of analytes as they arrive from two independent inlets. The fluorescently labeled molecules are tracked before, during and after they enter the mixing region, and their reactions with each other are observed by means of optical readout such as F{\"o}rster Resonance Energy Transfer (FRET). Our method can be used for analyzing the kinetics of DNA annealing in a high-parallelized fashion.",

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AU - Mathwig, Klaus

AU - Fijen, Carel

AU - Fontana, Mattia

AU - Lemay, Serge G.

AU - Hohlbein, Johannes

PY - 2017

Y1 - 2017

N2 - We introduce a nanofluidic mixing device entirely fabricated in glass for the fluorescence detection of single molecules. The design consists of a nanochannel T-junction and allows the continuous monitoring of chemical or enzymatic reactions of analytes as they arrive from two independent inlets. The fluorescently labeled molecules are tracked before, during and after they enter the mixing region, and their reactions with each other are observed by means of optical readout such as Förster Resonance Energy Transfer (FRET). Our method can be used for analyzing the kinetics of DNA annealing in a high-parallelized fashion.

AB - We introduce a nanofluidic mixing device entirely fabricated in glass for the fluorescence detection of single molecules. The design consists of a nanochannel T-junction and allows the continuous monitoring of chemical or enzymatic reactions of analytes as they arrive from two independent inlets. The fluorescently labeled molecules are tracked before, during and after they enter the mixing region, and their reactions with each other are observed by means of optical readout such as Förster Resonance Energy Transfer (FRET). Our method can be used for analyzing the kinetics of DNA annealing in a high-parallelized fashion.

KW - nanochannel

KW - nanofluidic mixing

KW - fluorescence detection

KW - single molecules

U2 - 10.1016/j.protcy.2017.04.062

DO - 10.1016/j.protcy.2017.04.062

M3 - Article

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VL - 27

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EP - 142

JO - Procedia technology

JF - Procedia technology

ER -

A nanofluidic mixing device for high-throughput fluorescence sensing of single molecules

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Keywords

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