A nanofluidic mixing device for high-throughput fluorescence sensing of single molecules

Klaus Mathwig, Carel Fijen, Mattia Fontana, Serge G. Lemay, Johannes Hohlbein

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

We introduce a nanofluidic mixing device entirely fabricated in glass for the fluorescence detection of single molecules. The design consists of a nanochannel T-junction and allows the continuous monitoring of chemical or enzymatic reactions of analytes as they arrive from two independent inlets. The fluorescently labeled molecules are tracked before, during and after they enter the mixing region, and their reactions with each other are observed by means of optical readout such as Förster Resonance Energy Transfer (FRET). Our method can be used for analyzing the kinetics of DNA annealing in a high-parallelized fashion.
Original languageEnglish
Pages (from-to)141-142
JournalProcedia technology
Volume27
DOIs
Publication statusPublished - 2017

Keywords

  • nanochannel
  • nanofluidic mixing
  • fluorescence detection
  • single molecules

Cite this

Mathwig, Klaus ; Fijen, Carel ; Fontana, Mattia ; Lemay, Serge G. ; Hohlbein, Johannes. / A nanofluidic mixing device for high-throughput fluorescence sensing of single molecules. In: Procedia technology. 2017 ; Vol. 27. pp. 141-142.
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A nanofluidic mixing device for high-throughput fluorescence sensing of single molecules. / Mathwig, Klaus; Fijen, Carel; Fontana, Mattia; Lemay, Serge G.; Hohlbein, Johannes.

In: Procedia technology, Vol. 27, 2017, p. 141-142.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Fontana, Mattia

AU - Lemay, Serge G.

AU - Hohlbein, Johannes

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AB - We introduce a nanofluidic mixing device entirely fabricated in glass for the fluorescence detection of single molecules. The design consists of a nanochannel T-junction and allows the continuous monitoring of chemical or enzymatic reactions of analytes as they arrive from two independent inlets. The fluorescently labeled molecules are tracked before, during and after they enter the mixing region, and their reactions with each other are observed by means of optical readout such as Förster Resonance Energy Transfer (FRET). Our method can be used for analyzing the kinetics of DNA annealing in a high-parallelized fashion.

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KW - fluorescence detection

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