A nerve stimulation method to selectively recruit smaller motor-units in rat skeletal muscle

A.I. van Bolhuis, J. Holsheimer, H.H.C.M. Savelsberg

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    23 Citations (Scopus)

    Abstract

    Electrical stimulation of peripheral nerve results in a motor-unit recruitment order opposite to that attained by natural neural control, i.e. from large, fast-fatiguing to progressively smaller, fatigue-resistant motor-units. Yet animal studies involving physiological exercise protocols of low intensity and long duration require minimal fatigue. The present study sought to apply a nerve stimulation method to selectively recruit smaller motor-units in rat skeletal muscle. Two pulse generators were used, independently supplying short supramaximal cathodal stimulating pulses (0.5 ms) and long subthreshold cathodal inactivating pulses (1.5 s) to the sciatic nerve. Propagation of action potentials was selectively blocked in nerve fibres of different diameter by adjusting the strength of the inactivating current. A tensile-testing machine was used to gauge isometric muscle force of the plantaris and both heads of the gastrocnemius muscle. The order of motor-unit recruitment was estimated from twitch characteristics, i.e. peak force and relaxation time. The results showed prolonged relaxation at lower twitch peak forces as the intensity of the inactivating current increased, indicating a reduction of the number of large motor-units to force production. It is shown that the nerve stimulation method described is effective in mimicking physiological muscle control.
    Original languageUndefined
    Pages (from-to)87-92
    Number of pages6
    JournalJournal of neuroscience methods
    Volume107
    Issue number1-2
    DOIs
    Publication statusPublished - 2001

    Keywords

    • IR-74543
    • METIS-112021
    • Isometric muscle force
    • Relaxation time
    • Muscle twitch
    • Direct current blocking
    • Nerve stimulation
    • Motor-unit recruitment

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