This paper introduces a novel fluidic device based on syringe-driven flow of fluorescent species through a parallel array of nanochannels, in which the geometrical confinement enables long observation times of non-immobilized molecules. Extremely low flow rates are achieved by operating the array of nanochannels in parallel with a larger microchannel. The addition of a second microfluidic inlet allows for mixing different species in a well-defined volume, enabling the study of irreversible reactions such as DNA synthesis in real-time using single-molecule fluorescence resonance energy transfer. Devices are fabricated in glass with the purpose of high-throughput single-molecule fluorescence detection.
|Title of host publication||MicroTas|
|Publication status||Published - 27 Oct 2013|
|Event||17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013 - Freiburg, Germany|
Duration: 27 Oct 2013 → 31 Oct 2013
Conference number: 17
|Conference||17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013|
|Period||27/10/13 → 31/10/13|