A novel parallel nanomixer for high-throughput single-molecule fluorescence detection

Klaus Mathwig, Stefan Schlautmann, Serge Joseph Guy Lemay, J. Hohlbein

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Abstract

This paper introduces a novel fluidic device based on syringe-driven flow of fluorescent species through a parallel array of nanochannels, in which the geometrical confinement enables long observation times of non-immobilized molecules. Extremely low flow rates are achieved by operating the array of nanochannels in parallel with a larger microchannel. The addition of a second microfluidic inlet allows for mixing different species in a well-defined volume, enabling the study of irreversible reactions such as DNA synthesis in real-time using single-molecule fluorescence resonance energy transfer. Devices are fabricated in glass with the purpose of high-throughput single-molecule fluorescence detection.
Original languageEnglish
Title of host publicationMicroTas
Pages1385-1387
Publication statusPublished - 27 Oct 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013 - Freiburg, Germany
Duration: 27 Oct 201331 Oct 2013
Conference number: 17

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2013
Abbreviated titleMicroTAS
CountryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords

  • IR-89770
  • METIS-298694

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