A poly(D,L-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography

F.P.W. Melchels, Jan Feijen, Dirk W. Grijpma

Research output: Contribution to journalArticleAcademicpeer-review

220 Citations (Scopus)

Abstract

Porous polylactide constructs were prepared by stereolithography, for the first time without the use of reactive diluents. Star-shaped poly(d,l-lactide) oligomers with 2, 3 and 6 arms were synthesised, end-functionalised with methacryloyl chloride and photo-crosslinked in the presence of ethyl lactate as a non-reactive diluent. The molecular weights of the arms of the macromers were 0.2, 0.6, 1.1 and 5 kg/mol, allowing variation of the crosslink density of the resulting networks. Networks prepared from macromers of which the molecular weight per arm was 0.6 kg/mol or higher had good mechanical properties, similar to linear high-molecular weight poly(d,l-lactide). A resin based on a 2-armed poly(d,l-lactide) macromer with a molecular weight of 0.6 kg/mol per arm (75 wt%), ethyl lactate (19 wt%), photo-initiator (6 wt%), inhibitor and dye was prepared. Using this resin, films and computer-designed porous constructs were accurately fabricated by stereolithography. Pre-osteoblasts showed good adherence to these photo-crosslinked networks. The proliferation rate on these materials was comparable to that on high-molecular weight poly(d,l-lactide) and tissue culture polystyrene
Original languageEnglish
Pages (from-to)3801-3809
JournalBiomaterials
Volume30
Issue number23-24
DOIs
Publication statusPublished - 2009

Fingerprint

Tissue Scaffolds
Stereolithography
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Resins
Molecular Weight
Molecular weight
Tissue culture
Polystyrenes
Osteoblasts
Oligomers
Stars
Coloring Agents
Dyes
poly(lactide)
Mechanical properties
dilactide

Keywords

  • METIS-263350
  • EC Grant Agreement nr.: FP6/500465
  • IR-76381

Cite this

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title = "A poly(D,L-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography",
abstract = "Porous polylactide constructs were prepared by stereolithography, for the first time without the use of reactive diluents. Star-shaped poly(d,l-lactide) oligomers with 2, 3 and 6 arms were synthesised, end-functionalised with methacryloyl chloride and photo-crosslinked in the presence of ethyl lactate as a non-reactive diluent. The molecular weights of the arms of the macromers were 0.2, 0.6, 1.1 and 5 kg/mol, allowing variation of the crosslink density of the resulting networks. Networks prepared from macromers of which the molecular weight per arm was 0.6 kg/mol or higher had good mechanical properties, similar to linear high-molecular weight poly(d,l-lactide). A resin based on a 2-armed poly(d,l-lactide) macromer with a molecular weight of 0.6 kg/mol per arm (75 wt{\%}), ethyl lactate (19 wt{\%}), photo-initiator (6 wt{\%}), inhibitor and dye was prepared. Using this resin, films and computer-designed porous constructs were accurately fabricated by stereolithography. Pre-osteoblasts showed good adherence to these photo-crosslinked networks. The proliferation rate on these materials was comparable to that on high-molecular weight poly(d,l-lactide) and tissue culture polystyrene",
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A poly(D,L-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography. / Melchels, F.P.W.; Feijen, Jan; Grijpma, Dirk W.

In: Biomaterials, Vol. 30, No. 23-24, 2009, p. 3801-3809.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A poly(D,L-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography

AU - Melchels, F.P.W.

AU - Feijen, Jan

AU - Grijpma, Dirk W.

PY - 2009

Y1 - 2009

N2 - Porous polylactide constructs were prepared by stereolithography, for the first time without the use of reactive diluents. Star-shaped poly(d,l-lactide) oligomers with 2, 3 and 6 arms were synthesised, end-functionalised with methacryloyl chloride and photo-crosslinked in the presence of ethyl lactate as a non-reactive diluent. The molecular weights of the arms of the macromers were 0.2, 0.6, 1.1 and 5 kg/mol, allowing variation of the crosslink density of the resulting networks. Networks prepared from macromers of which the molecular weight per arm was 0.6 kg/mol or higher had good mechanical properties, similar to linear high-molecular weight poly(d,l-lactide). A resin based on a 2-armed poly(d,l-lactide) macromer with a molecular weight of 0.6 kg/mol per arm (75 wt%), ethyl lactate (19 wt%), photo-initiator (6 wt%), inhibitor and dye was prepared. Using this resin, films and computer-designed porous constructs were accurately fabricated by stereolithography. Pre-osteoblasts showed good adherence to these photo-crosslinked networks. The proliferation rate on these materials was comparable to that on high-molecular weight poly(d,l-lactide) and tissue culture polystyrene

AB - Porous polylactide constructs were prepared by stereolithography, for the first time without the use of reactive diluents. Star-shaped poly(d,l-lactide) oligomers with 2, 3 and 6 arms were synthesised, end-functionalised with methacryloyl chloride and photo-crosslinked in the presence of ethyl lactate as a non-reactive diluent. The molecular weights of the arms of the macromers were 0.2, 0.6, 1.1 and 5 kg/mol, allowing variation of the crosslink density of the resulting networks. Networks prepared from macromers of which the molecular weight per arm was 0.6 kg/mol or higher had good mechanical properties, similar to linear high-molecular weight poly(d,l-lactide). A resin based on a 2-armed poly(d,l-lactide) macromer with a molecular weight of 0.6 kg/mol per arm (75 wt%), ethyl lactate (19 wt%), photo-initiator (6 wt%), inhibitor and dye was prepared. Using this resin, films and computer-designed porous constructs were accurately fabricated by stereolithography. Pre-osteoblasts showed good adherence to these photo-crosslinked networks. The proliferation rate on these materials was comparable to that on high-molecular weight poly(d,l-lactide) and tissue culture polystyrene

KW - METIS-263350

KW - EC Grant Agreement nr.: FP6/500465

KW - IR-76381

U2 - 10.1016/j.biomaterials.2009.03.055

DO - 10.1016/j.biomaterials.2009.03.055

M3 - Article

VL - 30

SP - 3801

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JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 23-24

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