A possible use of melatonin in the dental field: Protein adsorption and in vitro cell response on coated titanium

Andreia Cerqueira, Francisco Romero-Gavilán*, Nuno Araújo-Gomes, Iñaki García-Arnáez, Cristina Martinez-Ramos, Seda Ozturan, Mikel Azkargorta, Félix Elortza, Mariló Gurruchaga, Julio Suay, Isabel Goñi

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)
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Melatonin (MLT) is widely known for regulating the circadian cycles and has been studied for its role in bone regeneration and inflammation. Its application as a coating for dental implants can condition the local microenvironment, affecting protein deposition on its surface and the cellular and tissue response. Using sol-gel coatings as a release vehicle for MLT, the aim of this work was to assess the potential of this molecule in improving the osseointegration and inflammatory responses of a titanium substrate. The materials obtained were physicochemically characterized (scanning electron microscopy, contact angle, roughness, Fourier-transform infrared spectroscopy, nuclear magnetic resonance, Si release, MLT liberation, and degradation) and studied in vitro with MC3T3-E1 osteoblastic cells and RAW264.7 macrophage cells. Although MLT application led to an increased gene expression of RUNX2 and BMP2 in 10MTL, it did not improve ALP activity. On the other hand, MLT-enriched sol-gel materials presented potential effects in the adsorption of proteins related to inflammation, coagulation and angiogenesis pathways depending on the dosage used. Using LC-MS/MS, protein adsorption patterns were studied after incubation with human serum. Proteins related to the complement systems (CO7, IC1, CO5, CO8A, and CO9) were less adsorbed in materials with MLT; on the other hand, proteins with functions in the coagulation and angiogenesis pathways, such as A2GL and PLMN, showed a significant adsorption pattern.

Original languageEnglish
Article number111262
JournalMaterials Science and Engineering C: Materials for Biological Applications
Publication statusPublished - Nov 2020


  • Coating
  • Hybrid sol-gel
  • Inflammation
  • N-acetyl-5-metoxy-tryptamine
  • Osseointegration
  • Proteomics
  • 22/2 OA procedure


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