TY - JOUR
T1 - A possible use of melatonin in the dental field
T2 - Protein adsorption and in vitro cell response on coated titanium
AU - Cerqueira, Andreia
AU - Romero-Gavilán, Francisco
AU - Araújo-Gomes, Nuno
AU - García-Arnáez, Iñaki
AU - Martinez-Ramos, Cristina
AU - Ozturan, Seda
AU - Azkargorta, Mikel
AU - Elortza, Félix
AU - Gurruchaga, Mariló
AU - Suay, Julio
AU - Goñi, Isabel
PY - 2020/11
Y1 - 2020/11
N2 - Melatonin (MLT) is widely known for regulating the circadian cycles and has been studied for its role in bone regeneration and inflammation. Its application as a coating for dental implants can condition the local microenvironment, affecting protein deposition on its surface and the cellular and tissue response. Using sol-gel coatings as a release vehicle for MLT, the aim of this work was to assess the potential of this molecule in improving the osseointegration and inflammatory responses of a titanium substrate. The materials obtained were physicochemically characterized (scanning electron microscopy, contact angle, roughness, Fourier-transform infrared spectroscopy, nuclear magnetic resonance, Si release, MLT liberation, and degradation) and studied in vitro with MC3T3-E1 osteoblastic cells and RAW264.7 macrophage cells. Although MLT application led to an increased gene expression of RUNX2 and BMP2 in 10MTL, it did not improve ALP activity. On the other hand, MLT-enriched sol-gel materials presented potential effects in the adsorption of proteins related to inflammation, coagulation and angiogenesis pathways depending on the dosage used. Using LC-MS/MS, protein adsorption patterns were studied after incubation with human serum. Proteins related to the complement systems (CO7, IC1, CO5, CO8A, and CO9) were less adsorbed in materials with MLT; on the other hand, proteins with functions in the coagulation and angiogenesis pathways, such as A2GL and PLMN, showed a significant adsorption pattern.
AB - Melatonin (MLT) is widely known for regulating the circadian cycles and has been studied for its role in bone regeneration and inflammation. Its application as a coating for dental implants can condition the local microenvironment, affecting protein deposition on its surface and the cellular and tissue response. Using sol-gel coatings as a release vehicle for MLT, the aim of this work was to assess the potential of this molecule in improving the osseointegration and inflammatory responses of a titanium substrate. The materials obtained were physicochemically characterized (scanning electron microscopy, contact angle, roughness, Fourier-transform infrared spectroscopy, nuclear magnetic resonance, Si release, MLT liberation, and degradation) and studied in vitro with MC3T3-E1 osteoblastic cells and RAW264.7 macrophage cells. Although MLT application led to an increased gene expression of RUNX2 and BMP2 in 10MTL, it did not improve ALP activity. On the other hand, MLT-enriched sol-gel materials presented potential effects in the adsorption of proteins related to inflammation, coagulation and angiogenesis pathways depending on the dosage used. Using LC-MS/MS, protein adsorption patterns were studied after incubation with human serum. Proteins related to the complement systems (CO7, IC1, CO5, CO8A, and CO9) were less adsorbed in materials with MLT; on the other hand, proteins with functions in the coagulation and angiogenesis pathways, such as A2GL and PLMN, showed a significant adsorption pattern.
KW - Coating
KW - Hybrid sol-gel
KW - Inflammation
KW - N-acetyl-5-metoxy-tryptamine
KW - Osseointegration
KW - Proteomics
KW - 22/2 OA procedure
UR - http://www.scopus.com/inward/record.url?scp=85087587986&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2020.111262
DO - 10.1016/j.msec.2020.111262
M3 - Article
C2 - 32806297
AN - SCOPUS:85087587986
SN - 0928-4931
VL - 116
JO - Materials Science and Engineering C: Materials for Biological Applications
JF - Materials Science and Engineering C: Materials for Biological Applications
M1 - 111262
ER -