In this study the cryo-unit is introduced as a new and useful instrument to investigate water-containing specimens with the SEM. Often water-containing biological specimens are studied, but in our case we used water-swollen polymer membranes. The results show that application of a cryo-unit permits the study of this material at low temperatures up to magnifications of about 10 000 times, while other techniques failed to give reproducible results.
Roesink, H. D. W., Koenhen, D. M., de Jongh, M. A., & Smolders, C. A. (1978). A rapid preparation technique for studying highly water-swollen membranes with a scanning electron microscope (SEM) supplied with a cryo-unit. Ultramicroscopy, 3, 325-328. https://doi.org/10.1016/S0304-3991(78)80042-4