A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels

Hoon Suk Rho, Henk-Willem Veltkamp, Danielle Baptista, Severine le Gac, Pamela Habibović

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

We present a simple and robust microfabrication technique to create three-dimensional (3D) tapered polydimethylsiloxane (PDMS) channels. Tapered photoresist structures were fabricated by single-step lithography and thermal reflowing process, and the topography of the structures was controlled by varying design parameters, e.g., channel widths and length. Two half funnel-shaped PDMS replicas were combined to realize a 3D funnelshaped PDMS structure. Human umbilical vein endothelial cells (HUVECs) were cultured in the 3D funnel channel to form an artificial vessel network. We believe that the 3D topographic structures with controlled curvatures would be useful in many scientific and engineering fields, including lab-on-a-chip devices and bioinspired platforms.
Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Pages1156-1157
Number of pages2
ISBN (Electronic)978-1-7334190-0-0
Publication statusPublished - 27 Oct 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019 - Congress Center Basel, Basel, Switzerland
Duration: 27 Oct 201931 Oct 2019
Conference number: 23
https://cbmsociety.org/microtas/microtas2019/

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019
Abbreviated titleMicroTAS 2019
CountrySwitzerland
CityBasel
Period27/10/1931/10/19
Internet address

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funnels
fabrication
lab-on-a-chip devices
veins
replicas
cultured cells
photoresists
vessels
topography
lithography
platforms
curvature
engineering

Cite this

Rho, H. S., Veltkamp, H-W., Baptista, D., le Gac, S., & Habibović, P. (2019). A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1156-1157)
Rho, Hoon Suk ; Veltkamp, Henk-Willem ; Baptista, Danielle ; le Gac, Severine ; Habibović, Pamela. / A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels. 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences. 2019. pp. 1156-1157
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title = "A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels",
abstract = "We present a simple and robust microfabrication technique to create three-dimensional (3D) tapered polydimethylsiloxane (PDMS) channels. Tapered photoresist structures were fabricated by single-step lithography and thermal reflowing process, and the topography of the structures was controlled by varying design parameters, e.g., channel widths and length. Two half funnel-shaped PDMS replicas were combined to realize a 3D funnelshaped PDMS structure. Human umbilical vein endothelial cells (HUVECs) were cultured in the 3D funnel channel to form an artificial vessel network. We believe that the 3D topographic structures with controlled curvatures would be useful in many scientific and engineering fields, including lab-on-a-chip devices and bioinspired platforms.",
author = "Rho, {Hoon Suk} and Henk-Willem Veltkamp and Danielle Baptista and {le Gac}, Severine and Pamela Habibović",
year = "2019",
month = "10",
day = "27",
language = "English",
pages = "1156--1157",
booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences",

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Rho, HS, Veltkamp, H-W, Baptista, D, le Gac, S & Habibović, P 2019, A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels. in 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences. pp. 1156-1157, 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019, Basel, Switzerland, 27/10/19.

A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels. / Rho, Hoon Suk; Veltkamp, Henk-Willem ; Baptista, Danielle; le Gac, Severine ; Habibović, Pamela.

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences. 2019. p. 1156-1157.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels

AU - Rho, Hoon Suk

AU - Veltkamp, Henk-Willem

AU - Baptista, Danielle

AU - le Gac, Severine

AU - Habibović, Pamela

PY - 2019/10/27

Y1 - 2019/10/27

N2 - We present a simple and robust microfabrication technique to create three-dimensional (3D) tapered polydimethylsiloxane (PDMS) channels. Tapered photoresist structures were fabricated by single-step lithography and thermal reflowing process, and the topography of the structures was controlled by varying design parameters, e.g., channel widths and length. Two half funnel-shaped PDMS replicas were combined to realize a 3D funnelshaped PDMS structure. Human umbilical vein endothelial cells (HUVECs) were cultured in the 3D funnel channel to form an artificial vessel network. We believe that the 3D topographic structures with controlled curvatures would be useful in many scientific and engineering fields, including lab-on-a-chip devices and bioinspired platforms.

AB - We present a simple and robust microfabrication technique to create three-dimensional (3D) tapered polydimethylsiloxane (PDMS) channels. Tapered photoresist structures were fabricated by single-step lithography and thermal reflowing process, and the topography of the structures was controlled by varying design parameters, e.g., channel widths and length. Two half funnel-shaped PDMS replicas were combined to realize a 3D funnelshaped PDMS structure. Human umbilical vein endothelial cells (HUVECs) were cultured in the 3D funnel channel to form an artificial vessel network. We believe that the 3D topographic structures with controlled curvatures would be useful in many scientific and engineering fields, including lab-on-a-chip devices and bioinspired platforms.

M3 - Conference contribution

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BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences

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Rho HS, Veltkamp H-W, Baptista D, le Gac S, Habibović P. A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences. 2019. p. 1156-1157