A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels

Hoon Suk Rho, Henk-Willem Veltkamp, Danielle Baptista, Severine le Gac, Pamela Habibovic

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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    Abstract

    We present a simple and robust microfabrication technique to create three-dimensional (3D) tapered polydimethylsiloxane (PDMS) channels. Tapered photoresist structures were fabricated by single-step lithography and thermal reflowing process, and the topography of the structures was controlled by varying design parameters, e.g., channel widths and length. Two half funnel-shaped PDMS replicas were combined to realize a 3D funnelshaped PDMS structure. Human umbilical vein endothelial cells (HUVECs) were cultured in the 3D funnel channel to form an artificial vessel network. We believe that the 3D topographic structures with controlled curvatures would be useful in many scientific and engineering fields, including lab-on-a-chip devices and bioinspired platforms.
    Original languageEnglish
    Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences
    Pages1156-1157
    Number of pages2
    ISBN (Electronic)978-1-7334190-0-0
    Publication statusPublished - 27 Oct 2019
    Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019 - Congress Center Basel, Basel, Switzerland
    Duration: 27 Oct 201931 Oct 2019
    Conference number: 23
    https://cbmsociety.org/microtas/microtas2019/

    Conference

    Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019
    Abbreviated titleMicroTAS 2019
    CountrySwitzerland
    CityBasel
    Period27/10/1931/10/19
    Internet address

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    funnels
    fabrication
    lab-on-a-chip devices
    veins
    replicas
    cultured cells
    photoresists
    vessels
    topography
    lithography
    platforms
    curvature
    engineering

    Cite this

    Rho, H. S., Veltkamp, H-W., Baptista, D., le Gac, S., & Habibovic, P. (2019). A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1156-1157)
    Rho, Hoon Suk ; Veltkamp, Henk-Willem ; Baptista, Danielle ; le Gac, Severine ; Habibovic, Pamela. / A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels. 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences. 2019. pp. 1156-1157
    @inproceedings{7e477ec7dc624d31a1cd19acca8ca719,
    title = "A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels",
    abstract = "We present a simple and robust microfabrication technique to create three-dimensional (3D) tapered polydimethylsiloxane (PDMS) channels. Tapered photoresist structures were fabricated by single-step lithography and thermal reflowing process, and the topography of the structures was controlled by varying design parameters, e.g., channel widths and length. Two half funnel-shaped PDMS replicas were combined to realize a 3D funnelshaped PDMS structure. Human umbilical vein endothelial cells (HUVECs) were cultured in the 3D funnel channel to form an artificial vessel network. We believe that the 3D topographic structures with controlled curvatures would be useful in many scientific and engineering fields, including lab-on-a-chip devices and bioinspired platforms.",
    author = "Rho, {Hoon Suk} and Henk-Willem Veltkamp and Danielle Baptista and {le Gac}, Severine and Pamela Habibovic",
    year = "2019",
    month = "10",
    day = "27",
    language = "English",
    pages = "1156--1157",
    booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences",

    }

    Rho, HS, Veltkamp, H-W, Baptista, D, le Gac, S & Habibovic, P 2019, A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels. in 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences. pp. 1156-1157, 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019, Basel, Switzerland, 27/10/19.

    A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels. / Rho, Hoon Suk; Veltkamp, Henk-Willem ; Baptista, Danielle; le Gac, Severine ; Habibovic, Pamela.

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences. 2019. p. 1156-1157.

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    TY - GEN

    T1 - A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels

    AU - Rho, Hoon Suk

    AU - Veltkamp, Henk-Willem

    AU - Baptista, Danielle

    AU - le Gac, Severine

    AU - Habibovic, Pamela

    PY - 2019/10/27

    Y1 - 2019/10/27

    N2 - We present a simple and robust microfabrication technique to create three-dimensional (3D) tapered polydimethylsiloxane (PDMS) channels. Tapered photoresist structures were fabricated by single-step lithography and thermal reflowing process, and the topography of the structures was controlled by varying design parameters, e.g., channel widths and length. Two half funnel-shaped PDMS replicas were combined to realize a 3D funnelshaped PDMS structure. Human umbilical vein endothelial cells (HUVECs) were cultured in the 3D funnel channel to form an artificial vessel network. We believe that the 3D topographic structures with controlled curvatures would be useful in many scientific and engineering fields, including lab-on-a-chip devices and bioinspired platforms.

    AB - We present a simple and robust microfabrication technique to create three-dimensional (3D) tapered polydimethylsiloxane (PDMS) channels. Tapered photoresist structures were fabricated by single-step lithography and thermal reflowing process, and the topography of the structures was controlled by varying design parameters, e.g., channel widths and length. Two half funnel-shaped PDMS replicas were combined to realize a 3D funnelshaped PDMS structure. Human umbilical vein endothelial cells (HUVECs) were cultured in the 3D funnel channel to form an artificial vessel network. We believe that the 3D topographic structures with controlled curvatures would be useful in many scientific and engineering fields, including lab-on-a-chip devices and bioinspired platforms.

    M3 - Conference contribution

    SP - 1156

    EP - 1157

    BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences

    ER -

    Rho HS, Veltkamp H-W, Baptista D, le Gac S, Habibovic P. A simple and robust fabrication method for creating 3D tapered polydimethylsiloxane channels. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences. 2019. p. 1156-1157