A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors

Michiel van Leeuwen, Joseph J. Heijnen, Johannes G.E. Gardeniers, Arthur Oudshoorn, Henk Noorman, Jan Visser, Luuk A.M. van der Wielen, Walter M. van Gulik

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

A system has been developed, based on pressure controlled gas pumping, for accurate measurement of total gas consumption or production rates in the nmol/min range, applicable for on-line monitoring of bioconversions in microbioreactors. The system was validated by carrying out a bioconversion with known stoichiometric relation between gas consumption and substrate conversion, that is, the enzymatic oxidation of glucose to gluconic acid. The reaction was carried out in a stirred microreactor with a working volume of 100 μL, whereby the oxygen consumption was monitored on-line. Subsequently the system was applied to determine the oxygen transfer capacity of the microbioreactor. The dissolved oxygen concentration was measured with an optical dissolved oxygen sensor, which was integrated near the bottom of the reactor. Different stirrer sizes and geometries were investigated for their effect on the mass transfer of oxygen. A maximal kLa of 156±10 h−1, allowing a maximal O2-transfer rate up to 50 mmol O2/L/h, was reached which is sufficient to grow cells aerobically in (fed-)batch mode at relatively high biomass concentrations.
Original languageUndefined
Pages (from-to)455-458
Number of pages4
JournalChemical engineering science
Volume64
Issue number3
DOIs
Publication statusPublished - 2009

Keywords

  • Fermentation
  • Microbioreactor
  • Enzymatic reaction
  • IR-80126
  • Oxygen mass transfer
  • Pressure control
  • METIS-253169
  • Oxygen consumption

Cite this

van Leeuwen, Michiel ; Heijnen, Joseph J. ; Gardeniers, Johannes G.E. ; Oudshoorn, Arthur ; Noorman, Henk ; Visser, Jan ; van der Wielen, Luuk A.M. ; van Gulik, Walter M. / A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors. In: Chemical engineering science. 2009 ; Vol. 64, No. 3. pp. 455-458.
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abstract = "A system has been developed, based on pressure controlled gas pumping, for accurate measurement of total gas consumption or production rates in the nmol/min range, applicable for on-line monitoring of bioconversions in microbioreactors. The system was validated by carrying out a bioconversion with known stoichiometric relation between gas consumption and substrate conversion, that is, the enzymatic oxidation of glucose to gluconic acid. The reaction was carried out in a stirred microreactor with a working volume of 100 μL, whereby the oxygen consumption was monitored on-line. Subsequently the system was applied to determine the oxygen transfer capacity of the microbioreactor. The dissolved oxygen concentration was measured with an optical dissolved oxygen sensor, which was integrated near the bottom of the reactor. Different stirrer sizes and geometries were investigated for their effect on the mass transfer of oxygen. A maximal kLa of 156±10 h−1, allowing a maximal O2-transfer rate up to 50 mmol O2/L/h, was reached which is sufficient to grow cells aerobically in (fed-)batch mode at relatively high biomass concentrations.",
keywords = "Fermentation, Microbioreactor, Enzymatic reaction, IR-80126, Oxygen mass transfer, Pressure control, METIS-253169, Oxygen consumption",
author = "{van Leeuwen}, Michiel and Heijnen, {Joseph J.} and Gardeniers, {Johannes G.E.} and Arthur Oudshoorn and Henk Noorman and Jan Visser and {van der Wielen}, {Luuk A.M.} and {van Gulik}, {Walter M.}",
year = "2009",
doi = "10.1016/j.ces.2008.09.023",
language = "Undefined",
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pages = "455--458",
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van Leeuwen, M, Heijnen, JJ, Gardeniers, JGE, Oudshoorn, A, Noorman, H, Visser, J, van der Wielen, LAM & van Gulik, WM 2009, 'A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors', Chemical engineering science, vol. 64, no. 3, pp. 455-458. https://doi.org/10.1016/j.ces.2008.09.023

A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors. / van Leeuwen, Michiel; Heijnen, Joseph J.; Gardeniers, Johannes G.E.; Oudshoorn, Arthur; Noorman, Henk; Visser, Jan; van der Wielen, Luuk A.M.; van Gulik, Walter M.

In: Chemical engineering science, Vol. 64, No. 3, 2009, p. 455-458.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors

AU - van Leeuwen, Michiel

AU - Heijnen, Joseph J.

AU - Gardeniers, Johannes G.E.

AU - Oudshoorn, Arthur

AU - Noorman, Henk

AU - Visser, Jan

AU - van der Wielen, Luuk A.M.

AU - van Gulik, Walter M.

PY - 2009

Y1 - 2009

N2 - A system has been developed, based on pressure controlled gas pumping, for accurate measurement of total gas consumption or production rates in the nmol/min range, applicable for on-line monitoring of bioconversions in microbioreactors. The system was validated by carrying out a bioconversion with known stoichiometric relation between gas consumption and substrate conversion, that is, the enzymatic oxidation of glucose to gluconic acid. The reaction was carried out in a stirred microreactor with a working volume of 100 μL, whereby the oxygen consumption was monitored on-line. Subsequently the system was applied to determine the oxygen transfer capacity of the microbioreactor. The dissolved oxygen concentration was measured with an optical dissolved oxygen sensor, which was integrated near the bottom of the reactor. Different stirrer sizes and geometries were investigated for their effect on the mass transfer of oxygen. A maximal kLa of 156±10 h−1, allowing a maximal O2-transfer rate up to 50 mmol O2/L/h, was reached which is sufficient to grow cells aerobically in (fed-)batch mode at relatively high biomass concentrations.

AB - A system has been developed, based on pressure controlled gas pumping, for accurate measurement of total gas consumption or production rates in the nmol/min range, applicable for on-line monitoring of bioconversions in microbioreactors. The system was validated by carrying out a bioconversion with known stoichiometric relation between gas consumption and substrate conversion, that is, the enzymatic oxidation of glucose to gluconic acid. The reaction was carried out in a stirred microreactor with a working volume of 100 μL, whereby the oxygen consumption was monitored on-line. Subsequently the system was applied to determine the oxygen transfer capacity of the microbioreactor. The dissolved oxygen concentration was measured with an optical dissolved oxygen sensor, which was integrated near the bottom of the reactor. Different stirrer sizes and geometries were investigated for their effect on the mass transfer of oxygen. A maximal kLa of 156±10 h−1, allowing a maximal O2-transfer rate up to 50 mmol O2/L/h, was reached which is sufficient to grow cells aerobically in (fed-)batch mode at relatively high biomass concentrations.

KW - Fermentation

KW - Microbioreactor

KW - Enzymatic reaction

KW - IR-80126

KW - Oxygen mass transfer

KW - Pressure control

KW - METIS-253169

KW - Oxygen consumption

U2 - 10.1016/j.ces.2008.09.023

DO - 10.1016/j.ces.2008.09.023

M3 - Article

VL - 64

SP - 455

EP - 458

JO - Chemical engineering science

JF - Chemical engineering science

SN - 0009-2509

IS - 3

ER -