A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors

Michiel van Leeuwen, Joseph J. Heijnen, Johannes G.E. Gardeniers, Arthur Oudshoorn, Henk Noorman, Jan Visser, Luuk A.M. van der Wielen, Walter M. van Gulik

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A system has been developed, based on pressure controlled gas pumping, for accurate measurement of total gas consumption or production rates in the nmol/min range, applicable for on-line monitoring of bioconversions in microbioreactors. The system was validated by carrying out a bioconversion with known stoichiometric relation between gas consumption and substrate conversion, that is, the enzymatic oxidation of glucose to gluconic acid. The reaction was carried out in a stirred microreactor with a working volume of 100 μL, whereby the oxygen consumption was monitored on-line. Subsequently the system was applied to determine the oxygen transfer capacity of the microbioreactor. The dissolved oxygen concentration was measured with an optical dissolved oxygen sensor, which was integrated near the bottom of the reactor. Different stirrer sizes and geometries were investigated for their effect on the mass transfer of oxygen. A maximal kLa of 156±10 h−1, allowing a maximal O2-transfer rate up to 50 mmol O2/L/h, was reached which is sufficient to grow cells aerobically in (fed-)batch mode at relatively high biomass concentrations.
Original languageUndefined
Pages (from-to)455-458
Number of pages4
JournalChemical engineering science
Issue number3
Publication statusPublished - 2009


  • Fermentation
  • Microbioreactor
  • Enzymatic reaction
  • IR-80126
  • Oxygen mass transfer
  • Pressure control
  • METIS-253169
  • Oxygen consumption

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