A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors

Michiel van Leeuwen; Joseph J. Heijnen; Johannes G.E. Gardeniers; Arthur Oudshoorn; Henk Noorman; Jan Visser; Luuk A.M. van der Wielen; Walter M. van Gulik

doi:10.1016/j.ces.2008.09.023

A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors

Michiel van Leeuwen, Joseph J. Heijnen, Johannes G.E. Gardeniers, Arthur Oudshoorn, Henk Noorman, Jan Visser, Luuk A.M. van der Wielen, Walter M. van Gulik

Research output: Contribution to journal › Article › Academic › peer-review

8 Citations (Scopus)

Abstract

A system has been developed, based on pressure controlled gas pumping, for accurate measurement of total gas consumption or production rates in the nmol/min range, applicable for on-line monitoring of bioconversions in microbioreactors. The system was validated by carrying out a bioconversion with known stoichiometric relation between gas consumption and substrate conversion, that is, the enzymatic oxidation of glucose to gluconic acid. The reaction was carried out in a stirred microreactor with a working volume of 100 μL, whereby the oxygen consumption was monitored on-line. Subsequently the system was applied to determine the oxygen transfer capacity of the microbioreactor. The dissolved oxygen concentration was measured with an optical dissolved oxygen sensor, which was integrated near the bottom of the reactor. Different stirrer sizes and geometries were investigated for their effect on the mass transfer of oxygen. A maximal kLa of 156±10 h−1, allowing a maximal O2-transfer rate up to 50 mmol O2/L/h, was reached which is sufficient to grow cells aerobically in (fed-)batch mode at relatively high biomass concentrations.

Original language	Undefined
Pages (from-to)	455-458
Number of pages	4
Journal	Chemical engineering science
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/j.ces.2008.09.023
Publication status	Published - 2009

Keywords

Fermentation
Microbioreactor
Enzymatic reaction
IR-80126
Oxygen mass transfer
Pressure control
METIS-253169
Oxygen consumption

Cite this

van Leeuwen, M., Heijnen, J. J., Gardeniers, J. G. E., Oudshoorn, A., Noorman, H., Visser, J., ... van Gulik, W. M. (2009). A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors. Chemical engineering science, 64(3), 455-458. https://doi.org/10.1016/j.ces.2008.09.023

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abstract = "A system has been developed, based on pressure controlled gas pumping, for accurate measurement of total gas consumption or production rates in the nmol/min range, applicable for on-line monitoring of bioconversions in microbioreactors. The system was validated by carrying out a bioconversion with known stoichiometric relation between gas consumption and substrate conversion, that is, the enzymatic oxidation of glucose to gluconic acid. The reaction was carried out in a stirred microreactor with a working volume of 100 μL, whereby the oxygen consumption was monitored on-line. Subsequently the system was applied to determine the oxygen transfer capacity of the microbioreactor. The dissolved oxygen concentration was measured with an optical dissolved oxygen sensor, which was integrated near the bottom of the reactor. Different stirrer sizes and geometries were investigated for their effect on the mass transfer of oxygen. A maximal kLa of 156±10 h−1, allowing a maximal O2-transfer rate up to 50 mmol O2/L/h, was reached which is sufficient to grow cells aerobically in (fed-)batch mode at relatively high biomass concentrations.",

keywords = "Fermentation, Microbioreactor, Enzymatic reaction, IR-80126, Oxygen mass transfer, Pressure control, METIS-253169, Oxygen consumption",

author = "{van Leeuwen}, Michiel and Heijnen, {Joseph J.} and Gardeniers, {Johannes G.E.} and Arthur Oudshoorn and Henk Noorman and Jan Visser and {van der Wielen}, {Luuk A.M.} and {van Gulik}, {Walter M.}",

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doi = "10.1016/j.ces.2008.09.023",

language = "Undefined",

volume = "64",

pages = "455--458",

journal = "Chemical engineering science",

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A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors. / van Leeuwen, Michiel; Heijnen, Joseph J.; Gardeniers, Johannes G.E.; Oudshoorn, Arthur; Noorman, Henk; Visser, Jan; van der Wielen, Luuk A.M.; van Gulik, Walter M.

In: Chemical engineering science, Vol. 64, No. 3, 2009, p. 455-458.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors

AU - van Leeuwen, Michiel

AU - Heijnen, Joseph J.

AU - Gardeniers, Johannes G.E.

AU - Oudshoorn, Arthur

AU - Noorman, Henk

AU - Visser, Jan

AU - van der Wielen, Luuk A.M.

AU - van Gulik, Walter M.

PY - 2009

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AB - A system has been developed, based on pressure controlled gas pumping, for accurate measurement of total gas consumption or production rates in the nmol/min range, applicable for on-line monitoring of bioconversions in microbioreactors. The system was validated by carrying out a bioconversion with known stoichiometric relation between gas consumption and substrate conversion, that is, the enzymatic oxidation of glucose to gluconic acid. The reaction was carried out in a stirred microreactor with a working volume of 100 μL, whereby the oxygen consumption was monitored on-line. Subsequently the system was applied to determine the oxygen transfer capacity of the microbioreactor. The dissolved oxygen concentration was measured with an optical dissolved oxygen sensor, which was integrated near the bottom of the reactor. Different stirrer sizes and geometries were investigated for their effect on the mass transfer of oxygen. A maximal kLa of 156±10 h−1, allowing a maximal O2-transfer rate up to 50 mmol O2/L/h, was reached which is sufficient to grow cells aerobically in (fed-)batch mode at relatively high biomass concentrations.

KW - Fermentation

KW - Microbioreactor

KW - Enzymatic reaction

KW - IR-80126

KW - Oxygen mass transfer

KW - Pressure control

KW - METIS-253169

KW - Oxygen consumption

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DO - 10.1016/j.ces.2008.09.023

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JO - Chemical engineering science

JF - Chemical engineering science

SN - 0009-2509

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Access to Document

10.1016/j.ces.2008.09.023