Abstract
| Original language | Undefined |
|---|---|
| Pages (from-to) | 455-458 |
| Number of pages | 4 |
| Journal | Chemical engineering science |
| Volume | 64 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2009 |
Keywords
- Fermentation
- Microbioreactor
- Enzymatic reaction
- IR-80126
- Oxygen mass transfer
- Pressure control
- METIS-253169
- Oxygen consumption
Cite this
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A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors. / van Leeuwen, Michiel; Heijnen, Joseph J.; Gardeniers, Johannes G.E.; Oudshoorn, Arthur; Noorman, Henk; Visser, Jan; van der Wielen, Luuk A.M.; van Gulik, Walter M.
In: Chemical engineering science, Vol. 64, No. 3, 2009, p. 455-458.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - A system for accurate on-line measurement of total gas consumption or production rates in microbioreactors
AU - van Leeuwen, Michiel
AU - Heijnen, Joseph J.
AU - Gardeniers, Johannes G.E.
AU - Oudshoorn, Arthur
AU - Noorman, Henk
AU - Visser, Jan
AU - van der Wielen, Luuk A.M.
AU - van Gulik, Walter M.
PY - 2009
Y1 - 2009
N2 - A system has been developed, based on pressure controlled gas pumping, for accurate measurement of total gas consumption or production rates in the nmol/min range, applicable for on-line monitoring of bioconversions in microbioreactors. The system was validated by carrying out a bioconversion with known stoichiometric relation between gas consumption and substrate conversion, that is, the enzymatic oxidation of glucose to gluconic acid. The reaction was carried out in a stirred microreactor with a working volume of 100 μL, whereby the oxygen consumption was monitored on-line. Subsequently the system was applied to determine the oxygen transfer capacity of the microbioreactor. The dissolved oxygen concentration was measured with an optical dissolved oxygen sensor, which was integrated near the bottom of the reactor. Different stirrer sizes and geometries were investigated for their effect on the mass transfer of oxygen. A maximal kLa of 156±10 h−1, allowing a maximal O2-transfer rate up to 50 mmol O2/L/h, was reached which is sufficient to grow cells aerobically in (fed-)batch mode at relatively high biomass concentrations.
AB - A system has been developed, based on pressure controlled gas pumping, for accurate measurement of total gas consumption or production rates in the nmol/min range, applicable for on-line monitoring of bioconversions in microbioreactors. The system was validated by carrying out a bioconversion with known stoichiometric relation between gas consumption and substrate conversion, that is, the enzymatic oxidation of glucose to gluconic acid. The reaction was carried out in a stirred microreactor with a working volume of 100 μL, whereby the oxygen consumption was monitored on-line. Subsequently the system was applied to determine the oxygen transfer capacity of the microbioreactor. The dissolved oxygen concentration was measured with an optical dissolved oxygen sensor, which was integrated near the bottom of the reactor. Different stirrer sizes and geometries were investigated for their effect on the mass transfer of oxygen. A maximal kLa of 156±10 h−1, allowing a maximal O2-transfer rate up to 50 mmol O2/L/h, was reached which is sufficient to grow cells aerobically in (fed-)batch mode at relatively high biomass concentrations.
KW - Fermentation
KW - Microbioreactor
KW - Enzymatic reaction
KW - IR-80126
KW - Oxygen mass transfer
KW - Pressure control
KW - METIS-253169
KW - Oxygen consumption
U2 - 10.1016/j.ces.2008.09.023
DO - 10.1016/j.ces.2008.09.023
M3 - Article
VL - 64
SP - 455
EP - 458
JO - Chemical engineering science
JF - Chemical engineering science
SN - 0009-2509
IS - 3
ER -