A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes

C. Rousou; P. Hoogenboom; K. A. van Overdam; G. Storm; J. Dorrestijn; E. Mastrobattista

doi:10.1016/j.exer.2019.02.003

A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes

C. Rousou, P. Hoogenboom, K. A. van Overdam, G. Storm, J. Dorrestijn, E. Mastrobattista^* (Corresponding Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

188 Downloads (Pure)

Abstract

Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.

Original language	English
Pages (from-to)	171-177
Number of pages	7
Journal	Experimental eye research
Volume	181
Early online date	5 Feb 2019
DOIs	https://doi.org/10.1016/j.exer.2019.02.003
Publication status	Published - 1 Apr 2019
Externally published	Yes

Keywords

Extracorporeal model
Ophthalmic artery
Perfused porcine eye model
Retinal perfusion

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10.1016/j.exer.2019.02.003Licence: CC BY

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title = "A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes",

abstract = "Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.",

keywords = "Extracorporeal model, Ophthalmic artery, Perfused porcine eye model, Retinal perfusion",

author = "C. Rousou and P. Hoogenboom and {van Overdam}, {K. A.} and G. Storm and J. Dorrestijn and E. Mastrobattista",

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AU - Rousou, C.

AU - Hoogenboom, P.

AU - van Overdam, K. A.

AU - Storm, G.

AU - Dorrestijn, J.

AU - Mastrobattista, E.

PY - 2019/4/1

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N2 - Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.

AB - Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.

KW - Extracorporeal model

KW - Ophthalmic artery

KW - Perfused porcine eye model

KW - Retinal perfusion

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