Abstract
Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.
| Original language | English |
|---|---|
| Pages (from-to) | 171-177 |
| Number of pages | 7 |
| Journal | Experimental eye research |
| Volume | 181 |
| Early online date | 5 Feb 2019 |
| DOIs | |
| Publication status | Published - 1 Apr 2019 |
| Externally published | Yes |
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Keywords
- Extracorporeal model
- Ophthalmic artery
- Perfused porcine eye model
- Retinal perfusion
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A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes. / Rousou, C.; Hoogenboom, P.; van Overdam, K. A.; Storm, G.; Dorrestijn, J.; Mastrobattista, E. (Corresponding Author).
In: Experimental eye research, Vol. 181, 01.04.2019, p. 171-177.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes
AU - Rousou, C.
AU - Hoogenboom, P.
AU - van Overdam, K. A.
AU - Storm, G.
AU - Dorrestijn, J.
AU - Mastrobattista, E.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.
AB - Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.
KW - Extracorporeal model
KW - Ophthalmic artery
KW - Perfused porcine eye model
KW - Retinal perfusion
UR - http://www.scopus.com/inward/record.url?scp=85061324191&partnerID=8YFLogxK
U2 - 10.1016/j.exer.2019.02.003
DO - 10.1016/j.exer.2019.02.003
M3 - Article
VL - 181
SP - 171
EP - 177
JO - Experimental eye research
JF - Experimental eye research
SN - 0014-4835
ER -