A two-gene methylation signature for the diagnosis of bladder cancer in urine

Judith Bosschieter, Jakko A. Nieuwenhuijzen, Anouk Hentschel, Annina P. Van Splunter, Loes I. Segerink, André N. Vis, Saskia M. Wilting, Birgit I. Lissenberg-Witte, R. Jeroen A Van Moorselaar, Renske D.M. Steenbergen (Corresponding Author)

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Aim: To analyze the potential of 14 cancer-associated genes, including six miRNAs, for bladder cancer (BC) diagnosis in urine. Patients & methods: DNA methylation levels of 14 genes were analyzed in urine of 72 BC patients and 75 healthy controls using quantitative methylation-specific PCR. Multivariate logistic regression analysis was used to determine an optimal marker panel. Results: Ten genes were significantly hypermethylated in BC patients. The GHSR/MAL combination showed the best diagnostic performance, reaching a sensitivity of 92% (95% CI: 86-99) and a specificity of 85% (95% CI: 76-94). Conclusion: We identified a novel two-gene panel with a high diagnostic accuracy for BC that can be applied in a noninvasive, urine-based test.

Original languageEnglish
Pages (from-to)337-347
Number of pages11
JournalEpigenomics
Volume11
Issue number3
DOIs
Publication statusPublished - 1 Feb 2019

Fingerprint

Urinary Bladder Neoplasms
Methylation
Urine
Genes
Neoplasm Genes
DNA Methylation
MicroRNAs
Logistic Models
Regression Analysis
Polymerase Chain Reaction

Keywords

  • biomarkers
  • methylation
  • molecular diagnostics
  • urinary bladder neoplasms
  • urine analysis

Cite this

Bosschieter, J., Nieuwenhuijzen, J. A., Hentschel, A., Van Splunter, A. P., Segerink, L. I., Vis, A. N., ... Steenbergen, R. D. M. (2019). A two-gene methylation signature for the diagnosis of bladder cancer in urine. Epigenomics, 11(3), 337-347. https://doi.org/10.2217/epi-2018-0094
Bosschieter, Judith ; Nieuwenhuijzen, Jakko A. ; Hentschel, Anouk ; Van Splunter, Annina P. ; Segerink, Loes I. ; Vis, André N. ; Wilting, Saskia M. ; Lissenberg-Witte, Birgit I. ; A Van Moorselaar, R. Jeroen ; Steenbergen, Renske D.M. / A two-gene methylation signature for the diagnosis of bladder cancer in urine. In: Epigenomics. 2019 ; Vol. 11, No. 3. pp. 337-347.
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abstract = "Aim: To analyze the potential of 14 cancer-associated genes, including six miRNAs, for bladder cancer (BC) diagnosis in urine. Patients & methods: DNA methylation levels of 14 genes were analyzed in urine of 72 BC patients and 75 healthy controls using quantitative methylation-specific PCR. Multivariate logistic regression analysis was used to determine an optimal marker panel. Results: Ten genes were significantly hypermethylated in BC patients. The GHSR/MAL combination showed the best diagnostic performance, reaching a sensitivity of 92{\%} (95{\%} CI: 86-99) and a specificity of 85{\%} (95{\%} CI: 76-94). Conclusion: We identified a novel two-gene panel with a high diagnostic accuracy for BC that can be applied in a noninvasive, urine-based test.",
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Bosschieter, J, Nieuwenhuijzen, JA, Hentschel, A, Van Splunter, AP, Segerink, LI, Vis, AN, Wilting, SM, Lissenberg-Witte, BI, A Van Moorselaar, RJ & Steenbergen, RDM 2019, 'A two-gene methylation signature for the diagnosis of bladder cancer in urine' Epigenomics, vol. 11, no. 3, pp. 337-347. https://doi.org/10.2217/epi-2018-0094

A two-gene methylation signature for the diagnosis of bladder cancer in urine. / Bosschieter, Judith; Nieuwenhuijzen, Jakko A.; Hentschel, Anouk; Van Splunter, Annina P.; Segerink, Loes I.; Vis, André N.; Wilting, Saskia M.; Lissenberg-Witte, Birgit I.; A Van Moorselaar, R. Jeroen; Steenbergen, Renske D.M. (Corresponding Author).

In: Epigenomics, Vol. 11, No. 3, 01.02.2019, p. 337-347.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - A two-gene methylation signature for the diagnosis of bladder cancer in urine

AU - Bosschieter, Judith

AU - Nieuwenhuijzen, Jakko A.

AU - Hentschel, Anouk

AU - Van Splunter, Annina P.

AU - Segerink, Loes I.

AU - Vis, André N.

AU - Wilting, Saskia M.

AU - Lissenberg-Witte, Birgit I.

AU - A Van Moorselaar, R. Jeroen

AU - Steenbergen, Renske D.M.

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N2 - Aim: To analyze the potential of 14 cancer-associated genes, including six miRNAs, for bladder cancer (BC) diagnosis in urine. Patients & methods: DNA methylation levels of 14 genes were analyzed in urine of 72 BC patients and 75 healthy controls using quantitative methylation-specific PCR. Multivariate logistic regression analysis was used to determine an optimal marker panel. Results: Ten genes were significantly hypermethylated in BC patients. The GHSR/MAL combination showed the best diagnostic performance, reaching a sensitivity of 92% (95% CI: 86-99) and a specificity of 85% (95% CI: 76-94). Conclusion: We identified a novel two-gene panel with a high diagnostic accuracy for BC that can be applied in a noninvasive, urine-based test.

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Bosschieter J, Nieuwenhuijzen JA, Hentschel A, Van Splunter AP, Segerink LI, Vis AN et al. A two-gene methylation signature for the diagnosis of bladder cancer in urine. Epigenomics. 2019 Feb 1;11(3):337-347. https://doi.org/10.2217/epi-2018-0094