A versatile electrophoresis-based self-test platform

S.S. Staal, Mathijn Ungerer, J. Floris, Hans-Willem ten Brinke, Roy Helmhout, Marian Tellegen, Kjeld Janssen, Erik Karstens, Charlotte van Arragon, S.O. Lenk, E. Staijen, Jody Bartholomew, Hans Krabbe, Kris Movig, P. Dubsky, Albert van den Berg, Jan C.T. Eijkel

    Research output: Contribution to journalArticleAcademicpeer-review

    12 Citations (Scopus)

    Abstract

    This paper reports on recent research creating a family of electrophoresis-based point of care devices for the determination of a wide range of ionic analytes in various sample matrices. These devices are based on a first version for the point-of-care measurement of Li+, reported in 2010 by Floris etal. (Lab Chip 2010, 10, 1799-1806). With respect to this device, significant improvements in accuracy, precision, detection limit, and reliability have been obtained especially by the use of multiple injections of one sample on a single chip and integrated data analysis. Internal and external validation by clinical laboratories for the determination of analytes in real patients by a self-test is reported. For Li+ in blood better precision than the standard clinical determination for Li+ was achieved. For Na+ in human urine the method was found to be within the clinical acceptability limits. In a veterinary application, Ca2+ and Mg2+ were determined in bovine blood by means of the same chip, but using a different platform. Finally, promising preliminary results are reported with the Medimate platform for the determination of creatinine in whole blood and quantification of both cations and anions through replicate measurements on the same sample with the same chip.
    Original languageUndefined
    Pages (from-to)712-721
    Number of pages10
    JournalElectrophoresis
    Volume36
    Issue number5
    DOIs
    Publication statusPublished - Mar 2015

    Keywords

    • EWI-26000
    • IR-95868
    • METIS-312594

    Cite this

    Staal, S. S., Ungerer, M., Floris, J., ten Brinke, H-W., Helmhout, R., Tellegen, M., ... Eijkel, J. C. T. (2015). A versatile electrophoresis-based self-test platform. Electrophoresis, 36(5), 712-721. https://doi.org/10.1002/elps.201400428
    Staal, S.S. ; Ungerer, Mathijn ; Floris, J. ; ten Brinke, Hans-Willem ; Helmhout, Roy ; Tellegen, Marian ; Janssen, Kjeld ; Karstens, Erik ; van Arragon, Charlotte ; Lenk, S.O. ; Staijen, E. ; Bartholomew, Jody ; Krabbe, Hans ; Movig, Kris ; Dubsky, P. ; van den Berg, Albert ; Eijkel, Jan C.T. / A versatile electrophoresis-based self-test platform. In: Electrophoresis. 2015 ; Vol. 36, No. 5. pp. 712-721.
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    abstract = "This paper reports on recent research creating a family of electrophoresis-based point of care devices for the determination of a wide range of ionic analytes in various sample matrices. These devices are based on a first version for the point-of-care measurement of Li+, reported in 2010 by Floris etal. (Lab Chip 2010, 10, 1799-1806). With respect to this device, significant improvements in accuracy, precision, detection limit, and reliability have been obtained especially by the use of multiple injections of one sample on a single chip and integrated data analysis. Internal and external validation by clinical laboratories for the determination of analytes in real patients by a self-test is reported. For Li+ in blood better precision than the standard clinical determination for Li+ was achieved. For Na+ in human urine the method was found to be within the clinical acceptability limits. In a veterinary application, Ca2+ and Mg2+ were determined in bovine blood by means of the same chip, but using a different platform. Finally, promising preliminary results are reported with the Medimate platform for the determination of creatinine in whole blood and quantification of both cations and anions through replicate measurements on the same sample with the same chip.",
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    author = "S.S. Staal and Mathijn Ungerer and J. Floris and {ten Brinke}, Hans-Willem and Roy Helmhout and Marian Tellegen and Kjeld Janssen and Erik Karstens and {van Arragon}, Charlotte and S.O. Lenk and E. Staijen and Jody Bartholomew and Hans Krabbe and Kris Movig and P. Dubsky and {van den Berg}, Albert and Eijkel, {Jan C.T.}",
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    Staal, SS, Ungerer, M, Floris, J, ten Brinke, H-W, Helmhout, R, Tellegen, M, Janssen, K, Karstens, E, van Arragon, C, Lenk, SO, Staijen, E, Bartholomew, J, Krabbe, H, Movig, K, Dubsky, P, van den Berg, A & Eijkel, JCT 2015, 'A versatile electrophoresis-based self-test platform', Electrophoresis, vol. 36, no. 5, pp. 712-721. https://doi.org/10.1002/elps.201400428

    A versatile electrophoresis-based self-test platform. / Staal, S.S.; Ungerer, Mathijn; Floris, J.; ten Brinke, Hans-Willem; Helmhout, Roy; Tellegen, Marian; Janssen, Kjeld; Karstens, Erik; van Arragon, Charlotte; Lenk, S.O.; Staijen, E.; Bartholomew, Jody; Krabbe, Hans; Movig, Kris; Dubsky, P.; van den Berg, Albert; Eijkel, Jan C.T.

    In: Electrophoresis, Vol. 36, No. 5, 03.2015, p. 712-721.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - Staal, S.S.

    AU - Ungerer, Mathijn

    AU - Floris, J.

    AU - ten Brinke, Hans-Willem

    AU - Helmhout, Roy

    AU - Tellegen, Marian

    AU - Janssen, Kjeld

    AU - Karstens, Erik

    AU - van Arragon, Charlotte

    AU - Lenk, S.O.

    AU - Staijen, E.

    AU - Bartholomew, Jody

    AU - Krabbe, Hans

    AU - Movig, Kris

    AU - Dubsky, P.

    AU - van den Berg, Albert

    AU - Eijkel, Jan C.T.

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    N2 - This paper reports on recent research creating a family of electrophoresis-based point of care devices for the determination of a wide range of ionic analytes in various sample matrices. These devices are based on a first version for the point-of-care measurement of Li+, reported in 2010 by Floris etal. (Lab Chip 2010, 10, 1799-1806). With respect to this device, significant improvements in accuracy, precision, detection limit, and reliability have been obtained especially by the use of multiple injections of one sample on a single chip and integrated data analysis. Internal and external validation by clinical laboratories for the determination of analytes in real patients by a self-test is reported. For Li+ in blood better precision than the standard clinical determination for Li+ was achieved. For Na+ in human urine the method was found to be within the clinical acceptability limits. In a veterinary application, Ca2+ and Mg2+ were determined in bovine blood by means of the same chip, but using a different platform. Finally, promising preliminary results are reported with the Medimate platform for the determination of creatinine in whole blood and quantification of both cations and anions through replicate measurements on the same sample with the same chip.

    AB - This paper reports on recent research creating a family of electrophoresis-based point of care devices for the determination of a wide range of ionic analytes in various sample matrices. These devices are based on a first version for the point-of-care measurement of Li+, reported in 2010 by Floris etal. (Lab Chip 2010, 10, 1799-1806). With respect to this device, significant improvements in accuracy, precision, detection limit, and reliability have been obtained especially by the use of multiple injections of one sample on a single chip and integrated data analysis. Internal and external validation by clinical laboratories for the determination of analytes in real patients by a self-test is reported. For Li+ in blood better precision than the standard clinical determination for Li+ was achieved. For Na+ in human urine the method was found to be within the clinical acceptability limits. In a veterinary application, Ca2+ and Mg2+ were determined in bovine blood by means of the same chip, but using a different platform. Finally, promising preliminary results are reported with the Medimate platform for the determination of creatinine in whole blood and quantification of both cations and anions through replicate measurements on the same sample with the same chip.

    KW - EWI-26000

    KW - IR-95868

    KW - METIS-312594

    U2 - 10.1002/elps.201400428

    DO - 10.1002/elps.201400428

    M3 - Article

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    Staal SS, Ungerer M, Floris J, ten Brinke H-W, Helmhout R, Tellegen M et al. A versatile electrophoresis-based self-test platform. Electrophoresis. 2015 Mar;36(5):712-721. https://doi.org/10.1002/elps.201400428