A versatile electrophoresis-based self-test platform

S.S. Staal, Mathijn Ungerer, J. Floris, Hans-Willem ten Brinke, Roy Helmhout, Marian Tellegen, Kjeld Janssen, Erik Karstens, Charlotte van Arragon, S.O. Lenk, E. Staijen, Jody Bartholomew, Hans Krabbe, Kris Movig, P. Dubsky, Albert van den Berg, Jan C.T. Eijkel

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

This paper reports on recent research creating a family of electrophoresis-based point of care devices for the determination of a wide range of ionic analytes in various sample matrices. These devices are based on a first version for the point-of-care measurement of Li+, reported in 2010 by Floris etal. (Lab Chip 2010, 10, 1799-1806). With respect to this device, significant improvements in accuracy, precision, detection limit, and reliability have been obtained especially by the use of multiple injections of one sample on a single chip and integrated data analysis. Internal and external validation by clinical laboratories for the determination of analytes in real patients by a self-test is reported. For Li+ in blood better precision than the standard clinical determination for Li+ was achieved. For Na+ in human urine the method was found to be within the clinical acceptability limits. In a veterinary application, Ca2+ and Mg2+ were determined in bovine blood by means of the same chip, but using a different platform. Finally, promising preliminary results are reported with the Medimate platform for the determination of creatinine in whole blood and quantification of both cations and anions through replicate measurements on the same sample with the same chip.
Original languageUndefined
Pages (from-to)712-721
Number of pages10
JournalElectrophoresis
Volume36
Issue number5
DOIs
Publication statusPublished - Mar 2015

Keywords

  • EWI-26000
  • IR-95868
  • METIS-312594

Cite this

Staal, S. S., Ungerer, M., Floris, J., ten Brinke, H-W., Helmhout, R., Tellegen, M., ... Eijkel, J. C. T. (2015). A versatile electrophoresis-based self-test platform. Electrophoresis, 36(5), 712-721. https://doi.org/10.1002/elps.201400428
Staal, S.S. ; Ungerer, Mathijn ; Floris, J. ; ten Brinke, Hans-Willem ; Helmhout, Roy ; Tellegen, Marian ; Janssen, Kjeld ; Karstens, Erik ; van Arragon, Charlotte ; Lenk, S.O. ; Staijen, E. ; Bartholomew, Jody ; Krabbe, Hans ; Movig, Kris ; Dubsky, P. ; van den Berg, Albert ; Eijkel, Jan C.T. / A versatile electrophoresis-based self-test platform. In: Electrophoresis. 2015 ; Vol. 36, No. 5. pp. 712-721.
@article{e963ed1fb1074a088ab9e526cfe2a9ce,
title = "A versatile electrophoresis-based self-test platform",
abstract = "This paper reports on recent research creating a family of electrophoresis-based point of care devices for the determination of a wide range of ionic analytes in various sample matrices. These devices are based on a first version for the point-of-care measurement of Li+, reported in 2010 by Floris etal. (Lab Chip 2010, 10, 1799-1806). With respect to this device, significant improvements in accuracy, precision, detection limit, and reliability have been obtained especially by the use of multiple injections of one sample on a single chip and integrated data analysis. Internal and external validation by clinical laboratories for the determination of analytes in real patients by a self-test is reported. For Li+ in blood better precision than the standard clinical determination for Li+ was achieved. For Na+ in human urine the method was found to be within the clinical acceptability limits. In a veterinary application, Ca2+ and Mg2+ were determined in bovine blood by means of the same chip, but using a different platform. Finally, promising preliminary results are reported with the Medimate platform for the determination of creatinine in whole blood and quantification of both cations and anions through replicate measurements on the same sample with the same chip.",
keywords = "EWI-26000, IR-95868, METIS-312594",
author = "S.S. Staal and Mathijn Ungerer and J. Floris and {ten Brinke}, Hans-Willem and Roy Helmhout and Marian Tellegen and Kjeld Janssen and Erik Karstens and {van Arragon}, Charlotte and S.O. Lenk and E. Staijen and Jody Bartholomew and Hans Krabbe and Kris Movig and P. Dubsky and {van den Berg}, Albert and Eijkel, {Jan C.T.}",
note = "eemcs-eprint-26000",
year = "2015",
month = "3",
doi = "10.1002/elps.201400428",
language = "Undefined",
volume = "36",
pages = "712--721",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "5",

}

Staal, SS, Ungerer, M, Floris, J, ten Brinke, H-W, Helmhout, R, Tellegen, M, Janssen, K, Karstens, E, van Arragon, C, Lenk, SO, Staijen, E, Bartholomew, J, Krabbe, H, Movig, K, Dubsky, P, van den Berg, A & Eijkel, JCT 2015, 'A versatile electrophoresis-based self-test platform' Electrophoresis, vol. 36, no. 5, pp. 712-721. https://doi.org/10.1002/elps.201400428

A versatile electrophoresis-based self-test platform. / Staal, S.S.; Ungerer, Mathijn; Floris, J.; ten Brinke, Hans-Willem; Helmhout, Roy; Tellegen, Marian; Janssen, Kjeld; Karstens, Erik; van Arragon, Charlotte; Lenk, S.O.; Staijen, E.; Bartholomew, Jody; Krabbe, Hans; Movig, Kris; Dubsky, P.; van den Berg, Albert; Eijkel, Jan C.T.

In: Electrophoresis, Vol. 36, No. 5, 03.2015, p. 712-721.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - A versatile electrophoresis-based self-test platform

AU - Staal, S.S.

AU - Ungerer, Mathijn

AU - Floris, J.

AU - ten Brinke, Hans-Willem

AU - Helmhout, Roy

AU - Tellegen, Marian

AU - Janssen, Kjeld

AU - Karstens, Erik

AU - van Arragon, Charlotte

AU - Lenk, S.O.

AU - Staijen, E.

AU - Bartholomew, Jody

AU - Krabbe, Hans

AU - Movig, Kris

AU - Dubsky, P.

AU - van den Berg, Albert

AU - Eijkel, Jan C.T.

N1 - eemcs-eprint-26000

PY - 2015/3

Y1 - 2015/3

N2 - This paper reports on recent research creating a family of electrophoresis-based point of care devices for the determination of a wide range of ionic analytes in various sample matrices. These devices are based on a first version for the point-of-care measurement of Li+, reported in 2010 by Floris etal. (Lab Chip 2010, 10, 1799-1806). With respect to this device, significant improvements in accuracy, precision, detection limit, and reliability have been obtained especially by the use of multiple injections of one sample on a single chip and integrated data analysis. Internal and external validation by clinical laboratories for the determination of analytes in real patients by a self-test is reported. For Li+ in blood better precision than the standard clinical determination for Li+ was achieved. For Na+ in human urine the method was found to be within the clinical acceptability limits. In a veterinary application, Ca2+ and Mg2+ were determined in bovine blood by means of the same chip, but using a different platform. Finally, promising preliminary results are reported with the Medimate platform for the determination of creatinine in whole blood and quantification of both cations and anions through replicate measurements on the same sample with the same chip.

AB - This paper reports on recent research creating a family of electrophoresis-based point of care devices for the determination of a wide range of ionic analytes in various sample matrices. These devices are based on a first version for the point-of-care measurement of Li+, reported in 2010 by Floris etal. (Lab Chip 2010, 10, 1799-1806). With respect to this device, significant improvements in accuracy, precision, detection limit, and reliability have been obtained especially by the use of multiple injections of one sample on a single chip and integrated data analysis. Internal and external validation by clinical laboratories for the determination of analytes in real patients by a self-test is reported. For Li+ in blood better precision than the standard clinical determination for Li+ was achieved. For Na+ in human urine the method was found to be within the clinical acceptability limits. In a veterinary application, Ca2+ and Mg2+ were determined in bovine blood by means of the same chip, but using a different platform. Finally, promising preliminary results are reported with the Medimate platform for the determination of creatinine in whole blood and quantification of both cations and anions through replicate measurements on the same sample with the same chip.

KW - EWI-26000

KW - IR-95868

KW - METIS-312594

U2 - 10.1002/elps.201400428

DO - 10.1002/elps.201400428

M3 - Article

VL - 36

SP - 712

EP - 721

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 5

ER -

Staal SS, Ungerer M, Floris J, ten Brinke H-W, Helmhout R, Tellegen M et al. A versatile electrophoresis-based self-test platform. Electrophoresis. 2015 Mar;36(5):712-721. https://doi.org/10.1002/elps.201400428