We studied the occurrence of T-cell subpopulations for patients with B-cell chronic lymphocytic leukemia. The CD8+ population was divided into CD8+ suppressor (CD8a+) and CD8+ cytotoxic (CD8b+) lymphocytes using difference in orthogonal light scattering. Average CD4+/CD8+ratios determined for all patients were decreased. For individual patients this sometimes was not true. In contrast CD4+/CD8a+ ratios were markedly increased in all individual patients. The CD8+ lymphocytes appeared to consist mainly of CD8b+lymphocytes. Moreover the CD8b+/CD8+ ratio correlated with clinical stage: untreated patients (stage 0 of Rai) have smaller CD8b+/CD8+ ratios than patients with advanced stages of Rai.
Terstappen, L. W. M. M., de Grooth, B. G., van Berkel, W., & ten Napel, C. H. H. (1988). Abnormal distribution of CD8 subpopulation in B-chronic lymphocytic leukemia identified by flow cytometry. Leukemia Research, 12(7), 551-557. https://doi.org/10.1016/0145-2126(88)90084-7