Absolute sizing and label-free identification of extracellular vesicles by flow cytometry

Edwin van der Pol; Leonie de Rond; Frank A.W. Coumans; Elmar L. Gool; Anita N. Böing; Auguste Sturk; Rienk Nieuwland; Ton G. van Leeuwen

doi:10.1016/j.nano.2017.12.012

Absolute sizing and label-free identification of extracellular vesicles by flow cytometry

Edwin van der Pol^*, Leonie de Rond, Frank A.W. Coumans, Elmar L. Gool, Anita N. Böing, Auguste Sturk, Rienk Nieuwland, Ton G. van Leeuwen

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

114 Citations (Scopus)

2 Downloads (Pure)

Abstract

Blood contains extracellular vesicles (EVs), which are biological nanoparticles with clinical applications. In blood plasma, EVs are outnumbered by similar-sized lipoprotein particles (LPs), leading to controversial data such as non-specific binding of antibodies to LPs. Flow cytometry is a clinically applicable technique to characterize single EVs in body fluids. However, flow cytometry data have arbitrary units, impeding standardization, data comparison, and data interpretation, such as differentiation between EVs and LPs. Here we present a new method, named flow cytometry scatter ratio (Flow-SR), to relate the ambiguous light scattering signals of flow cytometry to the diameter and refractive index (RI) of single nanoparticles between 200-500 nm in diameter. Flow-SR enables label-free differentiation between EVs and LPs and improves data interpretation and comparison. Because Flow-SR is easy to implement, widely applicable, and more accurate and faster than existing techniques to size nanoparticles in suspension, Flow-SR has numerous applications in nanomedicine.

Original language	English
Pages (from-to)	801-810
Number of pages	10
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	14
Issue number	3
DOIs	https://doi.org/10.1016/j.nano.2017.12.012
Publication status	Published - Apr 2018
Externally published	Yes

Keywords

Drug delivery
exosomes
extracellular vesicles
flow cytometry
nanomedicine
nanoparticles

Access to Document

10.1016/j.nano.2017.12.012Licence: CC BY-NC-ND

1-s2.0-S1549963417305907-mainFinal published version, 693 KBLicence: CC BY-NC-ND

Cite this

@article{04ec5e4032fb46f29bbccc77fe2f8379,

title = "Absolute sizing and label-free identification of extracellular vesicles by flow cytometry",

abstract = "Blood contains extracellular vesicles (EVs), which are biological nanoparticles with clinical applications. In blood plasma, EVs are outnumbered by similar-sized lipoprotein particles (LPs), leading to controversial data such as non-specific binding of antibodies to LPs. Flow cytometry is a clinically applicable technique to characterize single EVs in body fluids. However, flow cytometry data have arbitrary units, impeding standardization, data comparison, and data interpretation, such as differentiation between EVs and LPs. Here we present a new method, named flow cytometry scatter ratio (Flow-SR), to relate the ambiguous light scattering signals of flow cytometry to the diameter and refractive index (RI) of single nanoparticles between 200-500 nm in diameter. Flow-SR enables label-free differentiation between EVs and LPs and improves data interpretation and comparison. Because Flow-SR is easy to implement, widely applicable, and more accurate and faster than existing techniques to size nanoparticles in suspension, Flow-SR has numerous applications in nanomedicine.",

keywords = "Drug delivery, exosomes, extracellular vesicles, flow cytometry, nanomedicine, nanoparticles",

author = "{van der Pol}, Edwin and {de Rond}, Leonie and Coumans, {Frank A.W.} and Gool, {Elmar L.} and B{\"o}ing, {Anita N.} and Auguste Sturk and Rienk Nieuwland and {van Leeuwen}, {Ton G.}",

note = "Publisher Copyright: {\textcopyright} 2018 The Author",

year = "2018",

month = apr,

doi = "10.1016/j.nano.2017.12.012",

language = "English",

volume = "14",

pages = "801--810",

journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",

issn = "1549-9634",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Absolute sizing and label-free identification of extracellular vesicles by flow cytometry

AU - van der Pol, Edwin

AU - de Rond, Leonie

AU - Coumans, Frank A.W.

AU - Gool, Elmar L.

AU - Böing, Anita N.

AU - Sturk, Auguste

AU - Nieuwland, Rienk

AU - van Leeuwen, Ton G.

PY - 2018/4

Y1 - 2018/4

N2 - Blood contains extracellular vesicles (EVs), which are biological nanoparticles with clinical applications. In blood plasma, EVs are outnumbered by similar-sized lipoprotein particles (LPs), leading to controversial data such as non-specific binding of antibodies to LPs. Flow cytometry is a clinically applicable technique to characterize single EVs in body fluids. However, flow cytometry data have arbitrary units, impeding standardization, data comparison, and data interpretation, such as differentiation between EVs and LPs. Here we present a new method, named flow cytometry scatter ratio (Flow-SR), to relate the ambiguous light scattering signals of flow cytometry to the diameter and refractive index (RI) of single nanoparticles between 200-500 nm in diameter. Flow-SR enables label-free differentiation between EVs and LPs and improves data interpretation and comparison. Because Flow-SR is easy to implement, widely applicable, and more accurate and faster than existing techniques to size nanoparticles in suspension, Flow-SR has numerous applications in nanomedicine.

AB - Blood contains extracellular vesicles (EVs), which are biological nanoparticles with clinical applications. In blood plasma, EVs are outnumbered by similar-sized lipoprotein particles (LPs), leading to controversial data such as non-specific binding of antibodies to LPs. Flow cytometry is a clinically applicable technique to characterize single EVs in body fluids. However, flow cytometry data have arbitrary units, impeding standardization, data comparison, and data interpretation, such as differentiation between EVs and LPs. Here we present a new method, named flow cytometry scatter ratio (Flow-SR), to relate the ambiguous light scattering signals of flow cytometry to the diameter and refractive index (RI) of single nanoparticles between 200-500 nm in diameter. Flow-SR enables label-free differentiation between EVs and LPs and improves data interpretation and comparison. Because Flow-SR is easy to implement, widely applicable, and more accurate and faster than existing techniques to size nanoparticles in suspension, Flow-SR has numerous applications in nanomedicine.

KW - Drug delivery

KW - exosomes

KW - extracellular vesicles

KW - flow cytometry

KW - nanomedicine

KW - nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=85042173870&partnerID=8YFLogxK

U2 - 10.1016/j.nano.2017.12.012

DO - 10.1016/j.nano.2017.12.012

M3 - Article

C2 - 29307842

AN - SCOPUS:85042173870

SN - 1549-9634

VL - 14

SP - 801

EP - 810

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

IS - 3

ER -

Absolute sizing and label-free identification of extracellular vesicles by flow cytometry

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this