Accelerated bone formation by biphasic calcium phosphate with a novel sub-micron surface topography

R. Duan, L. A. van Dijk, D. Barbieri, F. de Groot, H. Yuan, J. D. de Bruijn (Corresponding Author)

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Abstract

Osteoinductive calcium phosphate (CaP) bone grafts have equivalent performance to autografts in repairing critical-size bone defects. The osteoinductive potential of CaP is linked to the size of the surface topographical features. In the present study, two novel biphasic calcium phosphate (BCP) bone grafts were synthesised with either sub-micron- (BCP<µm) or micron-scale (BCPµm) needle-shaped surface topography and compared to dimensionally similar tricalcium phosphate (TCP) with grain-shaped surface structures (TCP<µm and TCPµm). To clarify the possible function of the surface morphology (needle-like vs. grain-like) in initiating bone formation, the four CaP test materials were physicochemically characterised and implanted for 12 weeks in the dorsal muscle of beagles. The sub-micron needle-shaped topography of BCP<µm triggered earlier bone formation (3-6 weeks) as compared to the grain-shaped surface topography of TCP<µm, which formed bone at 6-9 weeks. After 12 weeks, the amount of induced bone formation in both materials was equivalent, based on histomorphometry. The micron-sized needle-shaped surface topography of BCPµm led to limited formation of new bone tissue, whereas its counterpart, TCPµm with grain-shaped surface topography, failed to trigger de novo bone formation. The relative strength of the parameters affecting CaP-driven bone induction was as follows: surface feature size > surface feature morphology > substrate chemistry. BCP materials with needle-shaped sub-micron surface topography gave rise to accelerated bone formation and slower rate of resorption than a comparable TCP. These characteristics may be translated to improve bone healing in orthotopic defects.

Original languageEnglish
Pages (from-to)60-73
Number of pages14
JournalEuropean cells & materials
Volume37
DOIs
Publication statusPublished - 28 Jan 2019

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Calcium phosphate
Surface topography
Osteogenesis
Bone
Bone and Bones
Grafts
Transplants
Autografts
Defects
Needles
hydroxyapatite-beta tricalcium phosphate
Substrates
calcium phosphate

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Duan, R. ; van Dijk, L. A. ; Barbieri, D. ; de Groot, F. ; Yuan, H. ; de Bruijn, J. D. / Accelerated bone formation by biphasic calcium phosphate with a novel sub-micron surface topography. In: European cells & materials. 2019 ; Vol. 37. pp. 60-73.
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Accelerated bone formation by biphasic calcium phosphate with a novel sub-micron surface topography. / Duan, R.; van Dijk, L. A.; Barbieri, D.; de Groot, F.; Yuan, H.; de Bruijn, J. D. (Corresponding Author).

In: European cells & materials, Vol. 37, 28.01.2019, p. 60-73.

Research output: Contribution to journalArticleAcademicpeer-review

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