Acoustical Properties of Individual Liposome-Loaded Microbubbles

Ying Luan (Corresponding Author), Telli Faez, Erik Gelderblom, Ilya Skachkov, Bart Geers, Ine Lentacker, Ton van der Steen, Michel Versluis, Nico de Jong

Research output: Contribution to journalArticleAcademicpeer-review

43 Citations (Scopus)


A comparison between phospholipid-coated microbubbles with and without liposomes attached to the microbubble surface was performed using the ultra-high-speed imaging camera (Brandaris 128). We investigated 73 liposome-loaded microbubbles (loaded microbubbles) and 41 microbubbles without liposome loading (unloaded microbubbles) with a diameter ranging from 3–10 μm at frequencies ranging from 0.6–3.8 MHz and acoustic pressures ranging from 5–100 kPa. The experimental data showed nearly the same shell elasticity for the loaded and unloaded bubbles, but the shell viscosity was higher for loaded bubbles compared with unloaded bubbles. For loaded bubbles, a higher pressure threshold for the bubble vibrations was noticed. In addition, an “expansion-only” behavior was observed for up to 69% of the investigated loaded bubbles, which mostly occurred at low acoustic pressures (≤30 kPa). Finally, fluorescence imaging showed heterogeneity of liposome distributions of the loaded bubbles.
Original languageEnglish
Pages (from-to)2174-2185
JournalUltrasound in medicine and biology
Issue number12
Publication statusPublished - 2012


  • Liposome-loaded microbubbles
  • Drug delivery
  • Ultrasound contrast agents
  • Shell characterization


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