TY - JOUR
T1 - Acoustical Properties of Individual Liposome-Loaded Microbubbles
AU - Luan, Ying
AU - Faez, Telli
AU - Gelderblom, Erik
AU - Skachkov, Ilya
AU - Geers, Bart
AU - Lentacker, Ine
AU - van der Steen, Ton
AU - Versluis, Michel
AU - de Jong, Nico
PY - 2012
Y1 - 2012
N2 - A comparison between phospholipid-coated microbubbles with and without liposomes attached to the microbubble surface was performed using the ultra-high-speed imaging camera (Brandaris 128). We investigated 73 liposome-loaded microbubbles (loaded microbubbles) and 41 microbubbles without liposome loading (unloaded microbubbles) with a diameter ranging from 3–10 μm at frequencies ranging from 0.6–3.8 MHz and acoustic pressures ranging from 5–100 kPa. The experimental data showed nearly the same shell elasticity for the loaded and unloaded bubbles, but the shell viscosity was higher for loaded bubbles compared with unloaded bubbles. For loaded bubbles, a higher pressure threshold for the bubble vibrations was noticed. In addition, an “expansion-only” behavior was observed for up to 69% of the investigated loaded bubbles, which mostly occurred at low acoustic pressures (≤30 kPa). Finally, fluorescence imaging showed heterogeneity of liposome distributions of the loaded bubbles.
AB - A comparison between phospholipid-coated microbubbles with and without liposomes attached to the microbubble surface was performed using the ultra-high-speed imaging camera (Brandaris 128). We investigated 73 liposome-loaded microbubbles (loaded microbubbles) and 41 microbubbles without liposome loading (unloaded microbubbles) with a diameter ranging from 3–10 μm at frequencies ranging from 0.6–3.8 MHz and acoustic pressures ranging from 5–100 kPa. The experimental data showed nearly the same shell elasticity for the loaded and unloaded bubbles, but the shell viscosity was higher for loaded bubbles compared with unloaded bubbles. For loaded bubbles, a higher pressure threshold for the bubble vibrations was noticed. In addition, an “expansion-only” behavior was observed for up to 69% of the investigated loaded bubbles, which mostly occurred at low acoustic pressures (≤30 kPa). Finally, fluorescence imaging showed heterogeneity of liposome distributions of the loaded bubbles.
KW - Liposome-loaded microbubbles
KW - Drug delivery
KW - Ultrasound contrast agents
KW - Shell characterization
U2 - 10.1016/j.ultrasmedbio.2012.07.023
DO - 10.1016/j.ultrasmedbio.2012.07.023
M3 - Article
VL - 38
SP - 2174
EP - 2185
JO - Ultrasound in medicine and biology
JF - Ultrasound in medicine and biology
SN - 0301-5629
IS - 12
ER -