Adhesion and neurite development of cortical neurons on micropatterns of polyethylenimine and fluorcarbon

T.G. Ruardij, M.H. Goedbloed, Wim Rutten

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    Abstract

    This study aims on the preparation of isolated islands of cortical neurons on modified glass surfaces. Isolated islands of cortical neurons were obtained with a combination of neuron-adhesive polyethylenimine (PEI) and neuron-repellent plasma-deposited fluorocarbon (FC). Neurite development and fasciculation of interconnecting neurites between PEI-coated areas was studied. The patterns consisted of PEI-coated wells (diameter 150 ¿m) which were initially etched in a Fluorocarbon (FC) layer. The separation distance between the PEI-coated wells was varied from 10 up to 90 ¿m. This paper shows that the chemical patterns, prepared with a combination of polyethylenimine (PEI) and plasma deposited Fluorocarbon (FC), results in highly compliant patterns of adhering cortical neurons. Furthermore, it was shown that the occurrence of connecting neurite fascicles between neurons on PEI-coated wells is inversely proportional to the separation distance between the wells. Interconnecting fascicles were especially present on the pattern with a minimal separation distance (10 ¿m) between the PEI-coated wells. In contrast, interconnecting neurite fascicles were not observed on patterns with a minimal separation distance of 80 ¿m between the wells
    Original languageUndefined
    Title of host publication1st International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine & Biology
    Place of PublicationLyon, France
    PublisherIEEE
    Pages635-638
    Number of pages4
    ISBN (Print)0-7803-6603-4
    DOIs
    Publication statusPublished - 12 Oct 2000
    Event1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology 2000 - Lyon, France
    Duration: 12 Oct 200014 Oct 2000

    Publication series

    Name
    PublisherIEEE

    Other

    Other1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology 2000
    CountryFrance
    CityLyon
    Period12/10/0014/10/00

    Keywords

    • METIS-113318
    • IR-16433

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