Adhesion and patterning of cortical neurons on polyethylenimine and fluorcarbon-coated surfaces

T.G. Ruardij; M.H. Goedbloed; Wim Rutten

doi:10.1109/IEMBS.2000.898028

Adhesion and patterning of cortical neurons on polyethylenimine and fluorcarbon-coated surfaces

T.G. Ruardij, M.H. Goedbloed, Wim Rutten

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

In this study adhesion and patterning of cortical neurons on modified glass surfaces was investigated. Patterns of cortical neurons were prepared with a combination of polyethylenimine (PEI) and plasma-deposited fluorocarbon (FC). In addition neurite development and fasciculation of interconnecting neurites between PEI-coated areas was studied. The patterns consisted of PEI-coated circular holes (diameter 150 pm) which were initially etched in a Fluorocarbon (FC) layer. The separation distance between the PEI-coated circular holes was varied from 10 up to 90 pm. This paper shows that the chemical patterns, prepared with a combination of polyethylenimine (PEI) and plasma deposited Fluorocarbon (FC), results in highly compliant patterns of adhering cortical neurons. Furthermore it was shown that interconnecting neurite bundles between neurons on the PEI-coated circular holes were especially present on the pattern with a minimal separation distance (10 pm) between the PEI-coated circular holes. In contrast interconnecting neurite bundles were hardly observed on patterns with a maximal separation distance (90 pm) between the PEI-coated circular holes.

Original language	Undefined
Title of host publication	Proceedings 2000 World Congress on Medical Physics and Biomedical Engineering
Place of Publication	Chicago, USA
Publisher	IEEE
Pages	-
Number of pages	4
ISBN (Print)	0-7803-6468-6 C
DOIs	https://doi.org/10.1109/IEMBS.2000.898028
Publication status	Published - 23 Jul 2000
Event	22nd Annual International Conference of the IEEEEngineering in Medicine and Biology Society, 2000, Chicago, USA - Duration: 1 Jan 2000 → 1 Jan 2000

Publication series

Name
Publisher	IEEE
Volume	2

Other

Other	22nd Annual International Conference of the IEEEEngineering in Medicine and Biology Society, 2000, Chicago, USA
Period	1/01/00 → 1/01/00

Keywords

METIS-113319
IR-16434

Access to Document

10.1109/IEMBS.2000.898028

00898028

Cite this

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title = "Adhesion and patterning of cortical neurons on polyethylenimine and fluorcarbon-coated surfaces",

abstract = "In this study adhesion and patterning of cortical neurons on modified glass surfaces was investigated. Patterns of cortical neurons were prepared with a combination of polyethylenimine (PEI) and plasma-deposited fluorocarbon (FC). In addition neurite development and fasciculation of interconnecting neurites between PEI-coated areas was studied. The patterns consisted of PEI-coated circular holes (diameter 150 pm) which were initially etched in a Fluorocarbon (FC) layer. The separation distance between the PEI-coated circular holes was varied from 10 up to 90 pm. This paper shows that the chemical patterns, prepared with a combination of polyethylenimine (PEI) and plasma deposited Fluorocarbon (FC), results in highly compliant patterns of adhering cortical neurons. Furthermore it was shown that interconnecting neurite bundles between neurons on the PEI-coated circular holes were especially present on the pattern with a minimal separation distance (10 pm) between the PEI-coated circular holes. In contrast interconnecting neurite bundles were hardly observed on patterns with a maximal separation distance (90 pm) between the PEI-coated circular holes.",

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Ruardij, TG, Goedbloed, MH & Rutten, W 2000, Adhesion and patterning of cortical neurons on polyethylenimine and fluorcarbon-coated surfaces. in Proceedings 2000 World Congress on Medical Physics and Biomedical Engineering. IEEE, Chicago, USA, pp. -, 22nd Annual International Conference of the IEEEEngineering in Medicine and Biology Society, 2000, Chicago, USA, 1/01/00. https://doi.org/10.1109/IEMBS.2000.898028

Adhesion and patterning of cortical neurons on polyethylenimine and fluorcarbon-coated surfaces. / Ruardij, T.G.; Goedbloed, M.H.; Rutten, Wim.

Proceedings 2000 World Congress on Medical Physics and Biomedical Engineering. Chicago, USA : IEEE, 2000. p. -.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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N2 - In this study adhesion and patterning of cortical neurons on modified glass surfaces was investigated. Patterns of cortical neurons were prepared with a combination of polyethylenimine (PEI) and plasma-deposited fluorocarbon (FC). In addition neurite development and fasciculation of interconnecting neurites between PEI-coated areas was studied. The patterns consisted of PEI-coated circular holes (diameter 150 pm) which were initially etched in a Fluorocarbon (FC) layer. The separation distance between the PEI-coated circular holes was varied from 10 up to 90 pm. This paper shows that the chemical patterns, prepared with a combination of polyethylenimine (PEI) and plasma deposited Fluorocarbon (FC), results in highly compliant patterns of adhering cortical neurons. Furthermore it was shown that interconnecting neurite bundles between neurons on the PEI-coated circular holes were especially present on the pattern with a minimal separation distance (10 pm) between the PEI-coated circular holes. In contrast interconnecting neurite bundles were hardly observed on patterns with a maximal separation distance (90 pm) between the PEI-coated circular holes.

AB - In this study adhesion and patterning of cortical neurons on modified glass surfaces was investigated. Patterns of cortical neurons were prepared with a combination of polyethylenimine (PEI) and plasma-deposited fluorocarbon (FC). In addition neurite development and fasciculation of interconnecting neurites between PEI-coated areas was studied. The patterns consisted of PEI-coated circular holes (diameter 150 pm) which were initially etched in a Fluorocarbon (FC) layer. The separation distance between the PEI-coated circular holes was varied from 10 up to 90 pm. This paper shows that the chemical patterns, prepared with a combination of polyethylenimine (PEI) and plasma deposited Fluorocarbon (FC), results in highly compliant patterns of adhering cortical neurons. Furthermore it was shown that interconnecting neurite bundles between neurons on the PEI-coated circular holes were especially present on the pattern with a minimal separation distance (10 pm) between the PEI-coated circular holes. In contrast interconnecting neurite bundles were hardly observed on patterns with a maximal separation distance (90 pm) between the PEI-coated circular holes.

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