Adhesion and proliferation of human schwann cells on adhesive coatings

Carmen Lia A.-M. Vleggeert-Lankamp, Ana P. Pêgo, Egbert A.J.F. Lakke, Marga Deenen, Enrico Marani, Ralph T.W.M. Thomeer

    Research output: Contribution to journalArticleAcademicpeer-review

    52 Citations (Scopus)

    Abstract

    Attachment to and proliferation on the substrate are deemed important considerations when Schwann cells (SCs) are to be seeded in synthetic nerve grafts. Attachment is a prerequisite for the SCs to survive and fast proliferation will yield large numbers of SCs in a short time, which appears promising for stimulation of peripheral nerve regeneration. The aim of the present study was to compare the adhesion and proliferation of human Schwann cells (HSCs) on different substrates. The following were selected for their suitability as an internal coating of synthetic nerve grafts; the extracellular matrix proteins fibronectin, laminin and collagen type I and the poly-electrolytes poly( -lysine) (PDL) and poly(ethylene-imine) (PEI). On all coatings, attachment of HSCs was satisfactory and comparable, indicating that this factor is not a major consideration in choosing a suitable coating. Proliferation was best on fibronectin, laminin and PDL, and worst on collagen type I and PEI. Since nerve regeneration is enhanced by laminin and/or fibronectin, these are preferred as coatings for synthetic nerve grafts seeded with SCs.
    Original languageEnglish
    Pages (from-to)2741-2751
    JournalBiomaterials
    Volume25
    Issue number14
    DOIs
    Publication statusPublished - 2004

    Keywords

    • METIS-219235
    • Poly-electrodes
    • Proliferation
    • Human Schwann cell
    • Extracellular matrix proteins
    • Attachment
    • IR-71921

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