Adhesion and spreading of cultured endothelial cells on modified and unmodified poly(ethylene terephthalate): a morphological study

P.B. van Wachem, J.M. Schakenraad, J. Feijen, T. Beugeling, W.G. van Aken, E.H. Blaauw, P. Nieuwenhuis, I. Molenaar

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Abstract

The in vitro adhesion and spreading of human endothelial cells (HEC) on hydrophobic poly(ethylene terephthalate) (PETP) and moderately wettable tissue culture polyethylene terephthalate) (TCPETP) were studied with light microscopy and electron microscopy. Numbers of HEC adhering on TCPETP were always higher than those found on PETP. When cells were seeded in the presence of serum, extensive cell spreading on both PETP and TCPETP was observed after the first 30 min. Thereafter, spread cells appeared to withdraw from the PETP surface, resulting in irregularly shaped cells. Complete cell spreading occurred on TCPETP. Complete cell spreading also occurred on PETP and TCPETP when HEC had first been seeded from phosphate buffer solution and serum was supplied after 30 min. Furthermore, HEC spread on both PETP and TCPETP when the surfaces were precoated with protein(s), which promotes cell adhesion. However, when plasma was used for the coating, spread cells did not proliferate in a monolayer pattern. This study shows that TCPETP is, in general, a better surface for adhesion and proliferation of HEC than is PETP, suggesting that vascular prostheses with a TCPETP-like surface will perform better in vivo than prostheses made of PETP.
Original languageEnglish
Pages (from-to)532-539
JournalBiomaterials
Volume10
Issue number8
DOIs
Publication statusPublished - 1989

Fingerprint

Polyethylene Terephthalates
Endothelial cells
Polyethylene terephthalates
Cultured Cells
Adhesion
Endothelial Cells
Tissue culture
Prosthetics
Blood Vessel Prosthesis
Cell adhesion
Serum
Cell Adhesion

Keywords

  • endothelium
  • IR-70439
  • Cell adhesion
  • Poly(ethylene terephthalate)

Cite this

van Wachem, P.B. ; Schakenraad, J.M. ; Feijen, J. ; Beugeling, T. ; van Aken, W.G. ; Blaauw, E.H. ; Nieuwenhuis, P. ; Molenaar, I. / Adhesion and spreading of cultured endothelial cells on modified and unmodified poly(ethylene terephthalate): a morphological study. In: Biomaterials. 1989 ; Vol. 10, No. 8. pp. 532-539.
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abstract = "The in vitro adhesion and spreading of human endothelial cells (HEC) on hydrophobic poly(ethylene terephthalate) (PETP) and moderately wettable tissue culture polyethylene terephthalate) (TCPETP) were studied with light microscopy and electron microscopy. Numbers of HEC adhering on TCPETP were always higher than those found on PETP. When cells were seeded in the presence of serum, extensive cell spreading on both PETP and TCPETP was observed after the first 30 min. Thereafter, spread cells appeared to withdraw from the PETP surface, resulting in irregularly shaped cells. Complete cell spreading occurred on TCPETP. Complete cell spreading also occurred on PETP and TCPETP when HEC had first been seeded from phosphate buffer solution and serum was supplied after 30 min. Furthermore, HEC spread on both PETP and TCPETP when the surfaces were precoated with protein(s), which promotes cell adhesion. However, when plasma was used for the coating, spread cells did not proliferate in a monolayer pattern. This study shows that TCPETP is, in general, a better surface for adhesion and proliferation of HEC than is PETP, suggesting that vascular prostheses with a TCPETP-like surface will perform better in vivo than prostheses made of PETP.",
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van Wachem, PB, Schakenraad, JM, Feijen, J, Beugeling, T, van Aken, WG, Blaauw, EH, Nieuwenhuis, P & Molenaar, I 1989, 'Adhesion and spreading of cultured endothelial cells on modified and unmodified poly(ethylene terephthalate): a morphological study' Biomaterials, vol. 10, no. 8, pp. 532-539. https://doi.org/10.1016/0142-9612(89)90058-6

Adhesion and spreading of cultured endothelial cells on modified and unmodified poly(ethylene terephthalate): a morphological study. / van Wachem, P.B.; Schakenraad, J.M.; Feijen, J.; Beugeling, T.; van Aken, W.G.; Blaauw, E.H.; Nieuwenhuis, P.; Molenaar, I.

In: Biomaterials, Vol. 10, No. 8, 1989, p. 532-539.

Research output: Contribution to journalArticleAcademic

TY - JOUR

T1 - Adhesion and spreading of cultured endothelial cells on modified and unmodified poly(ethylene terephthalate): a morphological study

AU - van Wachem, P.B.

AU - Schakenraad, J.M.

AU - Feijen, J.

AU - Beugeling, T.

AU - van Aken, W.G.

AU - Blaauw, E.H.

AU - Nieuwenhuis, P.

AU - Molenaar, I.

PY - 1989

Y1 - 1989

N2 - The in vitro adhesion and spreading of human endothelial cells (HEC) on hydrophobic poly(ethylene terephthalate) (PETP) and moderately wettable tissue culture polyethylene terephthalate) (TCPETP) were studied with light microscopy and electron microscopy. Numbers of HEC adhering on TCPETP were always higher than those found on PETP. When cells were seeded in the presence of serum, extensive cell spreading on both PETP and TCPETP was observed after the first 30 min. Thereafter, spread cells appeared to withdraw from the PETP surface, resulting in irregularly shaped cells. Complete cell spreading occurred on TCPETP. Complete cell spreading also occurred on PETP and TCPETP when HEC had first been seeded from phosphate buffer solution and serum was supplied after 30 min. Furthermore, HEC spread on both PETP and TCPETP when the surfaces were precoated with protein(s), which promotes cell adhesion. However, when plasma was used for the coating, spread cells did not proliferate in a monolayer pattern. This study shows that TCPETP is, in general, a better surface for adhesion and proliferation of HEC than is PETP, suggesting that vascular prostheses with a TCPETP-like surface will perform better in vivo than prostheses made of PETP.

AB - The in vitro adhesion and spreading of human endothelial cells (HEC) on hydrophobic poly(ethylene terephthalate) (PETP) and moderately wettable tissue culture polyethylene terephthalate) (TCPETP) were studied with light microscopy and electron microscopy. Numbers of HEC adhering on TCPETP were always higher than those found on PETP. When cells were seeded in the presence of serum, extensive cell spreading on both PETP and TCPETP was observed after the first 30 min. Thereafter, spread cells appeared to withdraw from the PETP surface, resulting in irregularly shaped cells. Complete cell spreading occurred on TCPETP. Complete cell spreading also occurred on PETP and TCPETP when HEC had first been seeded from phosphate buffer solution and serum was supplied after 30 min. Furthermore, HEC spread on both PETP and TCPETP when the surfaces were precoated with protein(s), which promotes cell adhesion. However, when plasma was used for the coating, spread cells did not proliferate in a monolayer pattern. This study shows that TCPETP is, in general, a better surface for adhesion and proliferation of HEC than is PETP, suggesting that vascular prostheses with a TCPETP-like surface will perform better in vivo than prostheses made of PETP.

KW - endothelium

KW - IR-70439

KW - Cell adhesion

KW - Poly(ethylene terephthalate)

U2 - 10.1016/0142-9612(89)90058-6

DO - 10.1016/0142-9612(89)90058-6

M3 - Article

VL - 10

SP - 532

EP - 539

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 8

ER -