Adhesion of cultured human endothelial cells onto methacrylate polymers with varying surface wettability and charge

P.B. van Wachem, A.H. Hogt, T. Beugeling, J. Feijen, A. Bantjes, J.P. Detmers, W.G. van Aken

Research output: Contribution to journalArticleAcademic

310 Citations (Scopus)
111 Downloads (Pure)

Abstract

The adhesion of human endothelial cells (HEC) onto a series of well-characterized methacrylate polymer surfaces with varying wettabilities and surface charges was studied either in serum-containing (CMS) or in serum-free (CM) culture medium. HEC adhesion in CMS onto (co)polymers * of hydroxyethyl methacrylate (HEMA) and methyl methacrylate (MMA) was found to be optimal on the moderately wettable copolymer (mol ratio 25 HEMA/75 MMA). Positively-charged copolymers of HEMA or MMA with trimethylaminoethyl methacrylate-HCl salt (TMAEMA-Cl), both with mol ratios of 85/15 and a negatively-charged copolymer of MMA with methacrylic acid (MAA), mol ratio 85/15 , showed high numbers of adhering HEC. In CM, HEC adhered onto the three charged copolymers mentioned above, but neither onto the copolymer of HEMA and MAA (mol ratio 85/15) nor onto the HEMA/MMA co- and homopolymers. Complete cell spreading in CM was only observed on the positively-charged copolymers.
Original languageEnglish
Pages (from-to)323-328
JournalBiomaterials
Volume8
Issue number5
DOIs
Publication statusPublished - 1987

Keywords

  • Surface charge
  • methacrylate (co)polymers
  • cellular adhesion
  • surface wettability
  • cells (human endothelial)
  • IR-69924
  • Polymers

Fingerprint

Dive into the research topics of 'Adhesion of cultured human endothelial cells onto methacrylate polymers with varying surface wettability and charge'. Together they form a unique fingerprint.

Cite this