Aminefluorides are known to affect bacterial adhesion to enamel. In order to obtain information on the structure of adsorbed aminefluoride coatings, dihydroaminefluoride (AmF 297) and oleylaminefluoride (AmF 335) were adsorbed onto glass from solutions with concentrations up to 10 mM. Surface tensions of the solutions were measured at 25°C. After coating the glass surfaces, surface free energies, zeta potentials and adsorbed layer thicknesses were determined from contact angles, electrophoresis and ellipsometry, respectively. Surface free energies decreased after coating with both types of aminefluoride from 109 to _ 45 mJ m-*. Coating with only high concentrations (> 5 mA4) of AmF 297 again resulted in surface free energies above 100 mJ m-‘. Zeta potentials, originally - 45 mV, became positive after coating with both types of aminefluoride (approximately + 5 mV). Coating with only AmF 297 at concentrations above 5 mM gave zeta potentials of + 20 mV. Adsorbed layer thicknesses were in the monolayer range, though AmF 297 on its own clearly formed thicker layers at higher concentrations. Both surfactants showed a tendency to form micelles in solution at concentrations higher than 1 n&f. The results indicate that both aminefluorides adsorb with the positively charged, polar group towards the glass, but only AmF 297 can form double layers at higher concentrations. The second layer, however, adsorbs with the positively charged polar groups towards the solution and is bound to the first layer by relatively weak forces between the hydrocarbon chains. Gentle rinsing with distilled water is sufficient to remove the double layers. This paper shows how a combination of surface techniques can yield a detailed picture of the structure and orientation of adsorbed layers on solid substrata.