Aerobic batch cultivation in micro bioreactor with integrated electrochemical sensor array

M. van Leeuwen, E.E. Krommenhoek, J.J. Heijnen, Johannes G.E. Gardeniers, L.A.M. van der Wielen, W.M. van Gulik

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Aerobic batch cultivations of Candida utilis were carried out in two micro bioreactors with a working volume of 100 μL operated in parallel. The dimensions of the micro bioreactors were similar as the wells in a 96-well microtiter plate, to preserve compatibility with the current high-throughput cultivation systems. Each micro bioreactor was equipped with an electrochemical sensor array for the online measurement of temperature, pH, dissolved oxygen, and viable biomass concentration. Furthermore, the CO2 production rate was obtained from the online measurement of cumulative CO2 production during the cultivation. The online data obtained by the sensor array and the CO2 production measurements appeared to be very reproducible for all batch cultivations performed and were highly comparable to measurement results obtained during a similar aerobic batch cultivation carried out in a conventional 4L bench-scale bioreactor. Although the sensor chip certainly needs further improvement on some points, this work clearly shows the applicability of electrochemical sensor arrays for the monitoring of parallel micro-scale fermentations, e.g. using the 96-well microtiterplate format.
Original languageEnglish
Pages (from-to)293-300
Number of pages8
JournalBiotechnology progress
Volume26
Issue number1
DOIs
Publication statusPublished - 2010

Keywords

  • batch culture
  • Candida utilis
  • CO2 production
  • Micro-reactor
  • IR-72782
  • electrochemical sensor array

Fingerprint Dive into the research topics of 'Aerobic batch cultivation in micro bioreactor with integrated electrochemical sensor array'. Together they form a unique fingerprint.

  • Cite this

    van Leeuwen, M., Krommenhoek, E. E., Heijnen, J. J., Gardeniers, J. G. E., van der Wielen, L. A. M., & van Gulik, W. M. (2010). Aerobic batch cultivation in micro bioreactor with integrated electrochemical sensor array. Biotechnology progress, 26(1), 293-300. https://doi.org/10.1002/btpr.315