Aging affects the conformation of cholesterol in the human eye lens

J.J. Duindam, G.F.J.M. Vrensen, C. Otto, J. Greve

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    17 Citations (Scopus)


    The distribution of 3-β-OH-cholesterol along the optical axis of 20 human eye lenses of different ages was measured using confocal Raman microspectroscopy in combination with the 3-β-hydroxysteroid-specific probe filipin. This non-destructive technique allows a spatially very detailed study of the distribution of 3-β-OH-cholesterol in individual lenses. It revealed age-related changes of the 3-β-OH-cholesterol distribution along the optical axis. The 3-β-OH-cholesterol was found to be distributed asymmetrically along the optical axis and this distribution was found to change with age. The relative 3-β-OH-cholesterol content in the anterior part of the lens decreases significantly with increasing age compared with its equal posterior counterpart. Additionally all the lenses have a very low 3-β-OH-cholesterol content in the central part of their nuclear region, the area where the primary lens fibres are located.
    Original languageEnglish
    Pages (from-to)86-91
    Number of pages6
    JournalOphthalmic research
    Issue numberSuppl. 1
    Publication statusPublished - 1996


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