All-numerical noise filtering of fluorescence signals for achieving ultra-low limit of detection in biomedical applications

C. Dongre, Markus Pollnau, Hugo Hoekstra

    Research output: Contribution to journalArticleAcademicpeer-review

    5 Citations (Scopus)

    Abstract

    We present an all-numerical method for post-processing of the fluorescent signal as obtained from labeled molecules by capillary electrophoresis (CE) in an optofluidic chip, on the basis of data filtering in the Fourier domain. It is shown that the method outperforms the well-known lock-in amplification during experiments in the reduction of noise by a factor of square root (2). The method is illustrated using experimental data obtained during CE separation of molecules from a commercial DNA ladder with 17 fluorescently labeled molecules having different base-pair sizes. An improvement in signal-to-noise ratio by a factor of ~10 is achieved, resulting in a record-low limit of detection of 210 fM.
    Original languageUndefined
    Pages (from-to)1248-1251
    Number of pages4
    JournalAnalyst
    Volume136
    Issue number6
    DOIs
    Publication statusPublished - Mar 2011

    Keywords

    • IOMS-SNS: SENSORS
    • EWI-19925
    • IR-75870
    • METIS-277599
    • EC Grant Agreement nr.: FP6/034562

    Cite this

    Dongre, C. ; Pollnau, Markus ; Hoekstra, Hugo. / All-numerical noise filtering of fluorescence signals for achieving ultra-low limit of detection in biomedical applications. In: Analyst. 2011 ; Vol. 136, No. 6. pp. 1248-1251.
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    All-numerical noise filtering of fluorescence signals for achieving ultra-low limit of detection in biomedical applications. / Dongre, C.; Pollnau, Markus; Hoekstra, Hugo.

    In: Analyst, Vol. 136, No. 6, 03.2011, p. 1248-1251.

    Research output: Contribution to journalArticleAcademicpeer-review

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    T1 - All-numerical noise filtering of fluorescence signals for achieving ultra-low limit of detection in biomedical applications

    AU - Dongre, C.

    AU - Pollnau, Markus

    AU - Hoekstra, Hugo

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    AB - We present an all-numerical method for post-processing of the fluorescent signal as obtained from labeled molecules by capillary electrophoresis (CE) in an optofluidic chip, on the basis of data filtering in the Fourier domain. It is shown that the method outperforms the well-known lock-in amplification during experiments in the reduction of noise by a factor of square root (2). The method is illustrated using experimental data obtained during CE separation of molecules from a commercial DNA ladder with 17 fluorescently labeled molecules having different base-pair sizes. An improvement in signal-to-noise ratio by a factor of ~10 is achieved, resulting in a record-low limit of detection of 210 fM.

    KW - IOMS-SNS: SENSORS

    KW - EWI-19925

    KW - IR-75870

    KW - METIS-277599

    KW - EC Grant Agreement nr.: FP6/034562

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    JF - Analyst

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