TY - JOUR
T1 - Amine-reactive PEGylated nanoparticles for potential bioconjugation
AU - Delaittre, Guillaume
AU - Justribo-Hernandez, Gemma
AU - Nolte, Roeland J.M.
AU - Cornelissen, Jeroen Johannes Lambertus Maria
PY - 2011
Y1 - 2011
N2 - Water-dispersible PEGylated nanoparticles (NPs) presenting amine-reactive conjugation sites at their surfaces were synthesized and their ability to react with amines was demonstrated. An amphiphilic block copolymer bearing an N-succinimidyl ester at its water-soluble end was synthesized by the consecutive controlled radical polymerization of poly(ethylene glycol) methacrylate and styrene from a functional halide initiator. After purification of the copolymer, NPs of approximately 40 nm were obtained by a self-assembly process in water. The reactivity of the NPs was evidenced by reacting them with primary amines, including a fluorescent dye. The activated ester remained stable throughout all synthetic steps and a nearly quantitative coupling efficiency was obtained.
AB - Water-dispersible PEGylated nanoparticles (NPs) presenting amine-reactive conjugation sites at their surfaces were synthesized and their ability to react with amines was demonstrated. An amphiphilic block copolymer bearing an N-succinimidyl ester at its water-soluble end was synthesized by the consecutive controlled radical polymerization of poly(ethylene glycol) methacrylate and styrene from a functional halide initiator. After purification of the copolymer, NPs of approximately 40 nm were obtained by a self-assembly process in water. The reactivity of the NPs was evidenced by reacting them with primary amines, including a fluorescent dye. The activated ester remained stable throughout all synthetic steps and a nearly quantitative coupling efficiency was obtained.
KW - METIS-283474
KW - IR-104485
U2 - 10.1002/marc.201000365
DO - 10.1002/marc.201000365
M3 - Article
SN - 1022-1336
VL - 32
SP - 19
EP - 24
JO - Macromolecular rapid communications
JF - Macromolecular rapid communications
IS - 1
ER -