Amine-reactive PEGylated nanoparticles for potential bioconjugation

Guillaume Delaittre, Gemma Justribo-Hernandez, Roeland J.M. Nolte, Jeroen Johannes Lambertus Maria Cornelissen

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Water-dispersible PEGylated nanoparticles (NPs) presenting amine-reactive conjugation sites at their surfaces were synthesized and their ability to react with amines was demonstrated. An amphiphilic block copolymer bearing an N-succinimidyl ester at its water-soluble end was synthesized by the consecutive controlled radical polymerization of poly(ethylene glycol) methacrylate and styrene from a functional halide initiator. After purification of the copolymer, NPs of approximately 40 nm were obtained by a self-assembly process in water. The reactivity of the NPs was evidenced by reacting them with primary amines, including a fluorescent dye. The activated ester remained stable throughout all synthetic steps and a nearly quantitative coupling efficiency was obtained.
Original languageEnglish
Pages (from-to)19-24
Number of pages5
JournalMacromolecular rapid communications
Volume32
Issue number1
DOIs
Publication statusPublished - 2011

Keywords

  • METIS-283474
  • IR-104485

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