An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks

S. Xie, J. G.E. Gardeniers, Regina Luttge*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

1 Citation (Scopus)

Abstract

To investigate the hypothesis that dynamic nanoscale stimuli can influence central nerve systems functioning, in this paper we develop a chip based on polydimethylsiloxane (PDMS) soft lithography which exerts nanoscale mechanical loads on an in vitro neuronal network by microfluidic pneumatic deformation of a membrane. Initial analysis on Calcium ion (Ca2+) imaging within rat primary cortical networks shows increased Ca2+activities in the neuronal network after a stimuli was introduced, indicating that the neuronal networks respond to the mechanical stimulus.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherThe Chemical and Biological Microsystems Society
Pages495-496
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 - Convention Center Dublin, Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016
Conference number: 20
http://www.microtas2016.org/

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016
Abbreviated titleMicroTAS 2016
CountryIreland
CityDublin
Period9/10/1613/10/16
Internet address

Keywords

  • Actuator chip
  • Caimaging
  • Mechano-stimuli
  • Neuronal networks

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  • Cite this

    Xie, S., Gardeniers, J. G. E., & Luttge, R. (2016). An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 495-496). The Chemical and Biological Microsystems Society.