An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks

S. Xie; J. G.E. Gardeniers; Regina Luttge

An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks

S. Xie, J. G.E. Gardeniers, Regina Luttge^*

^*Corresponding author for this work

Mesoscale Chemical Systems

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

1 Citation (Scopus)

Abstract

To investigate the hypothesis that dynamic nanoscale stimuli can influence central nerve systems functioning, in this paper we develop a chip based on polydimethylsiloxane (PDMS) soft lithography which exerts nanoscale mechanical loads on an in vitro neuronal network by microfluidic pneumatic deformation of a membrane. Initial analysis on Calcium ion (Ca²⁺) imaging within rat primary cortical networks shows increased Ca²⁺activities in the neuronal network after a stimuli was introduced, indicating that the neuronal networks respond to the mechanical stimulus.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	The Chemical and Biological Microsystems Society
Pages	495-496
Number of pages	2
ISBN (Electronic)	9780979806490
Publication status	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 Conference number: 20 http://www.microtas2016.org/

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016
Abbreviated title	MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	9/10/16 → 13/10/16
Internet address	http://www.microtas2016.org/

Keywords

Actuator chip
Caimaging
Mechano-stimuli
Neuronal networks

Cite this

@inproceedings{7f1f5f1976d24053a66d11c67886d5aa,

title = "An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks",

abstract = "To investigate the hypothesis that dynamic nanoscale stimuli can influence central nerve systems functioning, in this paper we develop a chip based on polydimethylsiloxane (PDMS) soft lithography which exerts nanoscale mechanical loads on an in vitro neuronal network by microfluidic pneumatic deformation of a membrane. Initial analysis on Calcium ion (Ca2+) imaging within rat primary cortical networks shows increased Ca2+activities in the neuronal network after a stimuli was introduced, indicating that the neuronal networks respond to the mechanical stimulus.",

keywords = "Actuator chip, Caimaging, Mechano-stimuli, Neuronal networks",

author = "S. Xie and Gardeniers, \{J. G.E.\} and Regina Luttge",

year = "2016",

language = "English",

pages = "495--496",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

publisher = "The Chemical and Biological Microsystems Society",

note = "20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

url = "http://www.microtas2016.org/",

}

Xie, S, Gardeniers, JGE & Luttge, R 2016, An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. The Chemical and Biological Microsystems Society, pp. 495-496, 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016, Dublin, Ireland, 9/10/16.

An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks. / Xie, S.; Gardeniers, J. G.E.; Luttge, Regina.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. The Chemical and Biological Microsystems Society, 2016. p. 495-496.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks

AU - Xie, S.

AU - Gardeniers, J. G.E.

AU - Luttge, Regina

N1 - Conference code: 20

PY - 2016

Y1 - 2016

N2 - To investigate the hypothesis that dynamic nanoscale stimuli can influence central nerve systems functioning, in this paper we develop a chip based on polydimethylsiloxane (PDMS) soft lithography which exerts nanoscale mechanical loads on an in vitro neuronal network by microfluidic pneumatic deformation of a membrane. Initial analysis on Calcium ion (Ca2+) imaging within rat primary cortical networks shows increased Ca2+activities in the neuronal network after a stimuli was introduced, indicating that the neuronal networks respond to the mechanical stimulus.

AB - To investigate the hypothesis that dynamic nanoscale stimuli can influence central nerve systems functioning, in this paper we develop a chip based on polydimethylsiloxane (PDMS) soft lithography which exerts nanoscale mechanical loads on an in vitro neuronal network by microfluidic pneumatic deformation of a membrane. Initial analysis on Calcium ion (Ca2+) imaging within rat primary cortical networks shows increased Ca2+activities in the neuronal network after a stimuli was introduced, indicating that the neuronal networks respond to the mechanical stimulus.

KW - Actuator chip

KW - Caimaging

KW - Mechano-stimuli

KW - Neuronal networks

UR - https://www.scopus.com/pages/publications/85014218504

M3 - Conference contribution

AN - SCOPUS:85014218504

SP - 495

EP - 496

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - The Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016

Y2 - 9 October 2016 through 13 October 2016

ER -

An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks

Abstract

Conference

Keywords

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