An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer

Xavi Illa, Wim de Malsche, Johan G. Bomer, Johannes G.E. Gardeniers, Jan C.T. Eijkel, Joan Ramon Morante, Albert Romano-Rodriguez, Gert Desmet

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)

Abstract

The current paper describes the development and characterization of a pillar array chip that is constructed out of a sandwich of cyclo olefin polymer (COP) sheets. The silicon master of a 5 cm long pillar array was embossed into the COP, yielding 4.3 µm deep pillars of 15.3 µm diameter with an external porosity of 43 % and a well designed sidewall region to avoid side wall induced band broadening. A closed channel configuration was obtained by pressure assisted thermal bonding to a non-processed COP lid. Injection of coumarin dye plugs and detection with a fluorescence microscope showed very close agreement of this channel configuration to theoretical expectations in terms of band broadening. This agreement is due to the low taper, the optimized sidewall region and the excellent bonding quality between the two polymer sheets, even at the pillar area. Under non-retained conditions (pure methanol as mobile phase), plate heights as low as 4 µm were obtained. Under retained conditions, using the native hydrophobic properties of the COP channel (in 70/30 v/v water/methanol mixture as mobile phase), a minimum plate height of 6 µm was obtained. A 4 component separation was successfully achieved, demonstrating that COP is a cheap and efficient alternative for silicon and silica based liquid chromatography formats.
Original languageUndefined
Article number10.139/b818918h
Pages (from-to)1511-1516
Number of pages6
JournalLab on a chip
Volume9
Issue number11
DOIs
Publication statusPublished - 2 Feb 2009

Keywords

  • IR-68803
  • METIS-264219
  • EWI-16965

Cite this

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title = "An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer",
abstract = "The current paper describes the development and characterization of a pillar array chip that is constructed out of a sandwich of cyclo olefin polymer (COP) sheets. The silicon master of a 5 cm long pillar array was embossed into the COP, yielding 4.3 µm deep pillars of 15.3 µm diameter with an external porosity of 43 {\%} and a well designed sidewall region to avoid side wall induced band broadening. A closed channel configuration was obtained by pressure assisted thermal bonding to a non-processed COP lid. Injection of coumarin dye plugs and detection with a fluorescence microscope showed very close agreement of this channel configuration to theoretical expectations in terms of band broadening. This agreement is due to the low taper, the optimized sidewall region and the excellent bonding quality between the two polymer sheets, even at the pillar area. Under non-retained conditions (pure methanol as mobile phase), plate heights as low as 4 µm were obtained. Under retained conditions, using the native hydrophobic properties of the COP channel (in 70/30 v/v water/methanol mixture as mobile phase), a minimum plate height of 6 µm was obtained. A 4 component separation was successfully achieved, demonstrating that COP is a cheap and efficient alternative for silicon and silica based liquid chromatography formats.",
keywords = "IR-68803, METIS-264219, EWI-16965",
author = "Xavi Illa and {de Malsche}, Wim and Bomer, {Johan G.} and Gardeniers, {Johannes G.E.} and Eijkel, {Jan C.T.} and Morante, {Joan Ramon} and Albert Romano-Rodriguez and Gert Desmet",
year = "2009",
month = "2",
day = "2",
doi = "10.1039/B818918H",
language = "Undefined",
volume = "9",
pages = "1511--1516",
journal = "Lab on a chip",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "11",

}

An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer. / Illa, Xavi; de Malsche, Wim; Bomer, Johan G.; Gardeniers, Johannes G.E.; Eijkel, Jan C.T.; Morante, Joan Ramon; Romano-Rodriguez, Albert; Desmet, Gert.

In: Lab on a chip, Vol. 9, No. 11, 10.139/b818918h, 02.02.2009, p. 1511-1516.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - An array of ordered pillars with retentive properties for pressure-driven liquid chromatography fabricated directly from an unmodified cyclo olefin polymer

AU - Illa, Xavi

AU - de Malsche, Wim

AU - Bomer, Johan G.

AU - Gardeniers, Johannes G.E.

AU - Eijkel, Jan C.T.

AU - Morante, Joan Ramon

AU - Romano-Rodriguez, Albert

AU - Desmet, Gert

PY - 2009/2/2

Y1 - 2009/2/2

N2 - The current paper describes the development and characterization of a pillar array chip that is constructed out of a sandwich of cyclo olefin polymer (COP) sheets. The silicon master of a 5 cm long pillar array was embossed into the COP, yielding 4.3 µm deep pillars of 15.3 µm diameter with an external porosity of 43 % and a well designed sidewall region to avoid side wall induced band broadening. A closed channel configuration was obtained by pressure assisted thermal bonding to a non-processed COP lid. Injection of coumarin dye plugs and detection with a fluorescence microscope showed very close agreement of this channel configuration to theoretical expectations in terms of band broadening. This agreement is due to the low taper, the optimized sidewall region and the excellent bonding quality between the two polymer sheets, even at the pillar area. Under non-retained conditions (pure methanol as mobile phase), plate heights as low as 4 µm were obtained. Under retained conditions, using the native hydrophobic properties of the COP channel (in 70/30 v/v water/methanol mixture as mobile phase), a minimum plate height of 6 µm was obtained. A 4 component separation was successfully achieved, demonstrating that COP is a cheap and efficient alternative for silicon and silica based liquid chromatography formats.

AB - The current paper describes the development and characterization of a pillar array chip that is constructed out of a sandwich of cyclo olefin polymer (COP) sheets. The silicon master of a 5 cm long pillar array was embossed into the COP, yielding 4.3 µm deep pillars of 15.3 µm diameter with an external porosity of 43 % and a well designed sidewall region to avoid side wall induced band broadening. A closed channel configuration was obtained by pressure assisted thermal bonding to a non-processed COP lid. Injection of coumarin dye plugs and detection with a fluorescence microscope showed very close agreement of this channel configuration to theoretical expectations in terms of band broadening. This agreement is due to the low taper, the optimized sidewall region and the excellent bonding quality between the two polymer sheets, even at the pillar area. Under non-retained conditions (pure methanol as mobile phase), plate heights as low as 4 µm were obtained. Under retained conditions, using the native hydrophobic properties of the COP channel (in 70/30 v/v water/methanol mixture as mobile phase), a minimum plate height of 6 µm was obtained. A 4 component separation was successfully achieved, demonstrating that COP is a cheap and efficient alternative for silicon and silica based liquid chromatography formats.

KW - IR-68803

KW - METIS-264219

KW - EWI-16965

U2 - 10.1039/B818918H

DO - 10.1039/B818918H

M3 - Article

VL - 9

SP - 1511

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JO - Lab on a chip

JF - Lab on a chip

SN - 1473-0197

IS - 11

M1 - 10.139/b818918h

ER -