An electrochemical sensor for fast detection of wound infection based on myeloperoxidase activity

Martin Hajnsek, Doris Schiffer*, Daniel Harrich, Daniel Koller, Vanessa Verient, Job van der Palen, Andrea Heinzle, Barbara Binder, Eva Sigl, Frank Sinner, Georg M. Guebitz

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    32 Citations (Scopus)
    13 Downloads (Pure)


    Infection can lead to severe complications during wound healing. We have developed an electrochemical sensor for fast and simple detection of wound infection based on the quantification of myeloperoxidase activity as a marker for infection. Applicability of the enzyme was confirmed with a correlation study with silver standard wound diagnostics. Significant higher enzyme activities comparing non infected and infected wound fluids were determined (P = 0.01). To eliminate supplemental substrate addition, the chlorination activity of the enzyme - the formation of hypochlorous acid (HOCl) from chloride and hydrogen peroxide - was investigated in different wound fluids and correlated with the peroxidation activity measurements. Significant activity differences were likewise obtained (P = 0.01). Based on this we constructed an electrochemical hydrogen peroxide sensor system for the quantification of chlorination activity in wound fluids. Furthermore, immobilized glucose oxidase was integrated into the system to provide hydrogen peroxide required by myeloperoxidase. Infected wound fluids were indeed identified by using the sensor system quantifying the consumption of hydrogen peroxide consumed by myeloperoxidase. Thereby, immobilized glucose oxidase was shown to produce enough hydrogen peroxide for the myeloperoxidase reaction from glucose present in wound fluids. There is a strong need for a simple but effective sensor system to determine infections in wounds. This sensor measuring hydrogen peroxide consumption could effectively identify infected wound fluids based on the myeloperoxidase activity.

    Original languageEnglish
    Pages (from-to)265-274
    Number of pages10
    JournalSensors and Actuators B: Chemical
    Early online date4 Dec 2014
    Publication statusPublished - 31 Mar 2015


    • Amperometry
    • Biosensor
    • Diagnosis
    • Myeloperoxidase Wound infection
    • Sensor system
    • n/a OA procedure


    Dive into the research topics of 'An electrochemical sensor for fast detection of wound infection based on myeloperoxidase activity'. Together they form a unique fingerprint.

    Cite this