An image cytometer for resource-poor settings to monitor disease progression in HIV infection

Research output: Contribution to journalArticleAcademicpeer-review

28 Citations (Scopus)

Abstract

Background: For resource-poor countries, affordable methods are required for enumeration of CD4+ T lymphocytes of HIV-positive patients. For infants, additional determination of CD4/CD8 ratio is needed. - Methods: We determine the CD4+ and CD8+ T lymphocytes as the CD3+CD4+ and CD3+CD8+ population of blood cells. Target cells are CD3-immunomagnetically separated from the whole blood, and CD4-Phycoerythrin and CD8-PerCP immunofluorescently labeled. A point-of-care single platform image cytometer was developed to enumerate the target CD3+CD4+ and CD3+CD8+ populations. It has light-emitting diodes illumination, is fully computer-controlled, operates from a 12 V battery, and was designed to be cheap and easy-to-handle. Target cells are imaged on a CCD camera and enumerated by an image analysis algorithm. The cytometer outputs the absolute number of CD4+ and CD8+ T lymphocytes/l and CD4/CD8 ratio. - Results: The quality of the cell images obtained with the cytometer is sufficient for a reliable enumeration of target cells. The image cytometer achieves an accuracy of better than 10% in the range of 50-1700 cells/l. Analysis of blood samples from HIV patients yields a good agreement with the TruCount method for CD4 and CD8 count and CD4/CD8 ratio. - Conclusions: The image cytometer is affordable (component costs $3,000), compact (25 × 25 × 20 cm3), and uses disposable test materials, making it a good candidate to monitor progression of immunodeficiency disease in resource-poor settings.
Original languageUndefined
Pages (from-to)132-142
Number of pages11
JournalCytometry. Part A
VolumeA
Issue number3
DOIs
Publication statusPublished - 2007

Keywords

  • IR-60742
  • LED excitation of immunofluorescence
  • METIS-240635
  • Image cytometer
  • CD4 and CD8 enumeration
  • resource-poor settings
  • automated microscope
  • point-of-care HIV monitoring

Cite this

@article{d3c72f3037744816bf3fd1794b0ad9b7,
title = "An image cytometer for resource-poor settings to monitor disease progression in HIV infection",
abstract = "Background: For resource-poor countries, affordable methods are required for enumeration of CD4+ T lymphocytes of HIV-positive patients. For infants, additional determination of CD4/CD8 ratio is needed. - Methods: We determine the CD4+ and CD8+ T lymphocytes as the CD3+CD4+ and CD3+CD8+ population of blood cells. Target cells are CD3-immunomagnetically separated from the whole blood, and CD4-Phycoerythrin and CD8-PerCP immunofluorescently labeled. A point-of-care single platform image cytometer was developed to enumerate the target CD3+CD4+ and CD3+CD8+ populations. It has light-emitting diodes illumination, is fully computer-controlled, operates from a 12 V battery, and was designed to be cheap and easy-to-handle. Target cells are imaged on a CCD camera and enumerated by an image analysis algorithm. The cytometer outputs the absolute number of CD4+ and CD8+ T lymphocytes/l and CD4/CD8 ratio. - Results: The quality of the cell images obtained with the cytometer is sufficient for a reliable enumeration of target cells. The image cytometer achieves an accuracy of better than 10{\%} in the range of 50-1700 cells/l. Analysis of blood samples from HIV patients yields a good agreement with the TruCount method for CD4 and CD8 count and CD4/CD8 ratio. - Conclusions: The image cytometer is affordable (component costs $3,000), compact (25 × 25 × 20 cm3), and uses disposable test materials, making it a good candidate to monitor progression of immunodeficiency disease in resource-poor settings.",
keywords = "IR-60742, LED excitation of immunofluorescence, METIS-240635, Image cytometer, CD4 and CD8 enumeration, resource-poor settings, automated microscope, point-of-care HIV monitoring",
author = "Aurel Ymeti and X. Li and Bj{\"o}rn Lunter and Christian Breukers and Tibbe, {Arjan G.J.} and Terstappen, {Leonardus Wendelinus Mathias Marie} and Jan Greve",
year = "2007",
doi = "10.1002/cyto.a.20375",
language = "Undefined",
volume = "A",
pages = "132--142",
journal = "Cytometry. Part A",
issn = "1552-4922",
publisher = "Wiley-Liss Inc.",
number = "3",

}

An image cytometer for resource-poor settings to monitor disease progression in HIV infection. / Ymeti, Aurel; Li, X.; Lunter, Björn; Breukers, Christian; Tibbe, Arjan G.J.; Terstappen, Leonardus Wendelinus Mathias Marie; Greve, Jan.

In: Cytometry. Part A, Vol. A, No. 3, 2007, p. 132-142.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - An image cytometer for resource-poor settings to monitor disease progression in HIV infection

AU - Ymeti, Aurel

AU - Li, X.

AU - Lunter, Björn

AU - Breukers, Christian

AU - Tibbe, Arjan G.J.

AU - Terstappen, Leonardus Wendelinus Mathias Marie

AU - Greve, Jan

PY - 2007

Y1 - 2007

N2 - Background: For resource-poor countries, affordable methods are required for enumeration of CD4+ T lymphocytes of HIV-positive patients. For infants, additional determination of CD4/CD8 ratio is needed. - Methods: We determine the CD4+ and CD8+ T lymphocytes as the CD3+CD4+ and CD3+CD8+ population of blood cells. Target cells are CD3-immunomagnetically separated from the whole blood, and CD4-Phycoerythrin and CD8-PerCP immunofluorescently labeled. A point-of-care single platform image cytometer was developed to enumerate the target CD3+CD4+ and CD3+CD8+ populations. It has light-emitting diodes illumination, is fully computer-controlled, operates from a 12 V battery, and was designed to be cheap and easy-to-handle. Target cells are imaged on a CCD camera and enumerated by an image analysis algorithm. The cytometer outputs the absolute number of CD4+ and CD8+ T lymphocytes/l and CD4/CD8 ratio. - Results: The quality of the cell images obtained with the cytometer is sufficient for a reliable enumeration of target cells. The image cytometer achieves an accuracy of better than 10% in the range of 50-1700 cells/l. Analysis of blood samples from HIV patients yields a good agreement with the TruCount method for CD4 and CD8 count and CD4/CD8 ratio. - Conclusions: The image cytometer is affordable (component costs $3,000), compact (25 × 25 × 20 cm3), and uses disposable test materials, making it a good candidate to monitor progression of immunodeficiency disease in resource-poor settings.

AB - Background: For resource-poor countries, affordable methods are required for enumeration of CD4+ T lymphocytes of HIV-positive patients. For infants, additional determination of CD4/CD8 ratio is needed. - Methods: We determine the CD4+ and CD8+ T lymphocytes as the CD3+CD4+ and CD3+CD8+ population of blood cells. Target cells are CD3-immunomagnetically separated from the whole blood, and CD4-Phycoerythrin and CD8-PerCP immunofluorescently labeled. A point-of-care single platform image cytometer was developed to enumerate the target CD3+CD4+ and CD3+CD8+ populations. It has light-emitting diodes illumination, is fully computer-controlled, operates from a 12 V battery, and was designed to be cheap and easy-to-handle. Target cells are imaged on a CCD camera and enumerated by an image analysis algorithm. The cytometer outputs the absolute number of CD4+ and CD8+ T lymphocytes/l and CD4/CD8 ratio. - Results: The quality of the cell images obtained with the cytometer is sufficient for a reliable enumeration of target cells. The image cytometer achieves an accuracy of better than 10% in the range of 50-1700 cells/l. Analysis of blood samples from HIV patients yields a good agreement with the TruCount method for CD4 and CD8 count and CD4/CD8 ratio. - Conclusions: The image cytometer is affordable (component costs $3,000), compact (25 × 25 × 20 cm3), and uses disposable test materials, making it a good candidate to monitor progression of immunodeficiency disease in resource-poor settings.

KW - IR-60742

KW - LED excitation of immunofluorescence

KW - METIS-240635

KW - Image cytometer

KW - CD4 and CD8 enumeration

KW - resource-poor settings

KW - automated microscope

KW - point-of-care HIV monitoring

U2 - 10.1002/cyto.a.20375

DO - 10.1002/cyto.a.20375

M3 - Article

VL - A

SP - 132

EP - 142

JO - Cytometry. Part A

JF - Cytometry. Part A

SN - 1552-4922

IS - 3

ER -