Glycogen depleted skeletal muscle fibres can be distinguished from non-depleted fibres with the periodic acid Schiff (PAS) reaction. In this paper the method of Meijer (1968) for the histochemical demonstration of phosphorylase activity is described as an efficient technique to increase the contrast between both groups of fibres.
Wirtz, P., Wallinga, W., & Vermorken, A. J. M. (1983). An improved technique for the demonstration of glycogen depleted skeletal muscle fibres. Histochemistry, 79(1), 141-143. https://doi.org/10.1007/BF00494351